Optimized Centrifugal Partition Chromatography (CPC) Protocol for Isolation of Urease Inhibitors: Magnoflorine and Berberine from Berberis vulgaris Extracts DOI Creative Commons
Sylwia Nakonieczna, Katarzyna Suśniak,

Anna Bozhadze

и другие.

Separations, Год журнала: 2024, Номер 11(4), С. 94 - 94

Опубликована: Март 25, 2024

In recent years, an increasing interest in phytotherapy has been observed. Parallel to the research on total extracts of plant material, numerous studies activity single molecules derived from plants are being conducted address their mechanisms action and determine active doses eventual interactions. Despite this phenomenon, isolation individual compounds is a bottleneck due its difficulty cost. This work presents results careful optimization magnoflorine berberine (isoquinoline alkaloids) recovery commonly distributed shrub, Berberis vulgaris, growing Poland Georgia, using CPC. Both known for medicinal properties, which makes methodology important area research. Additionally, CPC ability isolate high-quality large quantities, it effective easy-to-commercialize method. For successful separation, biphasic solvent system composed hexane, butanol, ethanol, water ratio (3:12:4:16 v/v/v/v) was used ascending mode, together with flow rate 8 mL/min rotation speed 1600 rpm. The method selective both compounds, delivered good root stem plant. qualitative composition alkaloids studied determined by HPLC-ESI-QTOF-MS/MS confirmed presence berberine, magnoflorine, jatrorhizine, palmatine group isoquinolines. isolates, were subjected Helicobacter pylori growth inhibition assay urease test assess whether, next previously proved anticancer these characterized H. inhibition. MGN found exhibit inhibitory potential against (IC50 = 25 mg/L).

Язык: Английский

Critical review and future perspectives on the anticancer effects of Stephania tetrandra S. Moore DOI
Jia He, Yanyang Liu, Yueping Jiang

и другие.

Phytochemistry Reviews, Год журнала: 2025, Номер unknown

Опубликована: Март 17, 2025

Язык: Английский

Процитировано

0

Anticonvulsant effects of isopimpinellin and its interactions with classic antiseizure medications and borneol in the mouse tonic–clonic seizure model: an isobolographic transformation DOI Creative Commons
Jarogniew J. Łuszczki, Hubert Bojar, Katarzyna Jankiewicz

и другие.

Pharmacological Reports, Год журнала: 2023, Номер 75(6), С. 1533 - 1543

Опубликована: Окт. 11, 2023

Abstract Background Overwhelming evidence indicates that some naturally occurring coumarins and terpenes are widely used in folk medicine due to their various therapeutic effects affecting the brain. Antiseizure medications (ASMs) principal treatment option for epilepsy patients, although novel strategies based on substances intensively investigated. This study was aimed at determining influence of isopimpinellin (ISOP—a coumarin) when administered either separately or combination with borneol (BOR—a monoterpenoid), antiseizure potencies four classic ASMs (carbamazepine (CBZ), phenytoin (PHT), phenobarbital (PB), valproate (VPA)) mouse model maximal electroshock-induced (MES) tonic–clonic seizures. Materials Tonic–clonic seizures were evoked experimentally mice after systemic ( ip ) administration respective doses ISOP, BOR, ASMs. Interactions two-drug (ISOP + a ASM) three-drug BOR mixtures assessed isobolographically MES model. Results ISOP (administered alone) had no impact anticonvulsant Due isobolographic transformation data, VPA exerted an antagonistic interaction, whereas CBZ, PHT, PB produced additive interactions The combinations CBZ PHT interactions, while synergistic Conclusions most intriguing interaction VPA, which addition transition from antagonism synergy

Язык: Английский

Процитировано

6

Optimized Centrifugal Partition Chromatography (CPC) Protocol for Isolation of Urease Inhibitors: Magnoflorine and Berberine from Berberis vulgaris Extracts DOI Creative Commons
Sylwia Nakonieczna, Katarzyna Suśniak,

Anna Bozhadze

и другие.

Separations, Год журнала: 2024, Номер 11(4), С. 94 - 94

Опубликована: Март 25, 2024

In recent years, an increasing interest in phytotherapy has been observed. Parallel to the research on total extracts of plant material, numerous studies activity single molecules derived from plants are being conducted address their mechanisms action and determine active doses eventual interactions. Despite this phenomenon, isolation individual compounds is a bottleneck due its difficulty cost. This work presents results careful optimization magnoflorine berberine (isoquinoline alkaloids) recovery commonly distributed shrub, Berberis vulgaris, growing Poland Georgia, using CPC. Both known for medicinal properties, which makes methodology important area research. Additionally, CPC ability isolate high-quality large quantities, it effective easy-to-commercialize method. For successful separation, biphasic solvent system composed hexane, butanol, ethanol, water ratio (3:12:4:16 v/v/v/v) was used ascending mode, together with flow rate 8 mL/min rotation speed 1600 rpm. The method selective both compounds, delivered good root stem plant. qualitative composition alkaloids studied determined by HPLC-ESI-QTOF-MS/MS confirmed presence berberine, magnoflorine, jatrorhizine, palmatine group isoquinolines. isolates, were subjected Helicobacter pylori growth inhibition assay urease test assess whether, next previously proved anticancer these characterized H. inhibition. MGN found exhibit inhibitory potential against (IC50 = 25 mg/L).

Язык: Английский

Процитировано

1