Endothelial Dysfunction with Aging: Does Sex Matter? DOI Open Access

Jakub Jozue Wojtacha,

Barbara Morawin, Edyta Wawrzyniak-Gramacka

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(22), С. 12203 - 12203

Опубликована: Ноя. 13, 2024

Oxidative stress and inflammation accompany endothelial dysfunction that results from the excessive or uncontrolled production of reactive oxygen nitrogen species (RONS) in older adults. This study was designed to assess usefulness serum oxi-inflammatory component combinations vascular disease prediction prevention with regard sex. Women (n = 145) men 50) aged 72.2 ± 7.8 years participated this project. The females demonstrated elevated hydrogen peroxide (H2O2) nitric oxide (NO) responsible for intravascular low-density lipoprotein oxidation. NO generation enhanced women, but its bioavailability reduced, which expressed by a high 3-nitrotyrosine (3-NitroT) concentration. relation NO/3-NitroT (rs 0.811, p < 0.001) women −0.611, showed sex determines dysfunction. RONS simultaneously promoted regeneration, as ~1.5-fold increase circulating progenitor cells. Inflammation-specific variables, such neutrophil-to-lymphocyte ratio, systemic immune index, neutrophil-to-high-density (HDL) were reduced their diagnostic utility clinical prognosis dysfunction, especially C-reactive-protein-to-HDL ratio (AUC 0.980, specificity 94.7%, sensitivity 93.3%, OR 252, 95% CI 65–967, 0.001). is first have revealed sex-specific changes response, can generate risk cardiovascular events at an age.

Язык: Английский

An Overview of Advances in Rare Cancer Diagnosis and Treatment DOI Open Access

Grania Christyani,

Matthew Carswell,

Sisi Qin

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(2), С. 1201 - 1201

Опубликована: Янв. 18, 2024

Cancer stands as the leading global cause of mortality, with rare cancer comprising 230 distinct subtypes characterized by infrequent incidence. Despite inherent challenges in addressing diagnosis and treatment cancers due to their low occurrence rates, several biomedical breakthroughs have led significant advancement both areas. This review provides a comprehensive overview state-of-the-art diagnostic techniques that encompass new-generation sequencing multi-omics, coupled integration artificial intelligence machine learning, revolutionized diagnosis. In addition, this highlights latest innovations therapeutic options, immunotherapy, targeted therapy, transplantation, drug combination undergone clinical trials significantly contribute tumor remission overall survival patients. review, we summarize recent insights understanding pathophysiology, diagnosis, modalities, well faced development data interpretation development.

Язык: Английский

Процитировано

9
Vagus Nerve Stimulation: Novel Concept for the Treatment of Glioblastoma and Solid Cancers by Cytokine (Interleukin-6) Reduction, Attenuating the SASP, Enhancing Tumor Immunity DOI Creative Commons
Steven Brem

Brain Behavior & Immunity - Health, Год журнала: 2024, Номер 42, С. 100859 - 100859

Опубликована: Сен. 17, 2024

Язык: Английский

Процитировано

4
Farnesol Inhibits Cell Viability and Activates Apoptosis in Glioma C6 Cells via Downregulating Phosphoinositide 3-kinase (PI3K)/Protein Kinase B (AKT)/Mammalian Target of Rapamycin (mTOR) Pathway DOI Creative Commons
Chao Gao, Shu-Jem Su, Qi Guan

и другие.

Pharmacognosy Magazine, Год журнала: 2025, Номер unknown

Опубликована: Янв. 13, 2025

Background Gliomas are the most aggressive and devastating brain tumors, with a poor prognosis due to their complex etiology, diverse clinical manifestations, limited efficacy of existing treatment methods. Purpose The present work was conducted understand anticancer activities farnesol against glioma C6 cells. Methods effects on in vitro free radical scavenging were evaluated at various dosages (1–100 µM). A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test used assess influence growth cells nonmalignant Vero apoptotic levels investigated using 4′,6-diamidino-2-phenylindole (DAPI) dual staining assays. oxidative stress markers, inflammatory biomarkers, protein levels, phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target rapamycin (mTOR) pathway proteins assessed respective kits. Results results assays proved antioxidant properties farnesol. MTT assay findings demonstrated significant reduction cell viability following fluorescent onset apoptosis farnesol-treated Furthermore, effectively increased stress, reduced marker PI3K, AKT, mTOR expression also successfully downregulated by Conclusion current demonstrate that suppresses triggers Thus, it has capacity be an effective agent treat glioma. Nonetheless, additional is still necessary future determine specific molecular mechanisms involved farnesol-induced

Язык: Английский

Процитировано

0
Inflammatory cytokine profile in non-small cell lung Cancer (NSCLC) patients during early enhanced recovery after surgery (ERAS) period and its relation to hospital length of stay DOI
Yongxia Song, Chun Li, Yan Fang

и другие.

Cytokine, Год журнала: 2025, Номер 190, С. 156918 - 156918

Опубликована: Март 22, 2025

Язык: Английский

Процитировано

0
IL-8 Instructs Macrophage Identity in Lateral Ventricle Contacting Glioblastoma DOI
Stephanie Medina, Asa A. Brockman, Claire E. Cross

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 30, 2024

Adult IDH-wildtype glioblastoma (GBM) is a highly aggressive brain tumor with no established immunotherapy or targeted therapy. Recently, CD32 + HLA-DR hi macrophages were discovered to have displaced resident microglia in GBM tumors that contact the lateral ventricle stem cell niche. Since these contacting especially poor outcomes, identifying origin and role of likely critical developing successful immunotherapies. Here, we identify cells as M_IL-8 establish IL-8 sufficient necessary for instruct healthy into M2 macrophages. Using set 73 tumors, protein shown be present vivo common ventricle. Finally, ex experiments conditioned medium from primary human cells, inhibitory antibodies blocked generation cells. These results provide mechanistic predominate microenvironment most tumors. should now explored targets combination immunotherapies, patients whose radiographic ventricular subventricular zone

Язык: Английский

Процитировано

2
Diagnostic and Prognostic Value of Circulating DNA Fragments in Glioblastoma Multiforme Patients DOI Open Access
Paweł Jarmużek, Edyta Wawrzyniak-Gramacka, Barbara Morawin

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(8), С. 4221 - 4221

Опубликована: Апрель 11, 2024

Novel blood-circulating molecules, as potential biomarkers for glioblastoma multiforme (GBM) diagnosis and monitoring, are attracting particular attention due to limitations of imaging modalities invasive tissue biopsy procedures. This study aims assess the diagnostic prognostic values circulating cell-free DNA (cfDNA) in relation inflammatory status GBM patients determine concentration average size fragments typical tumour-derived fractions. Preoperative plasma samples from 40 (GBM 65.0 ± 11.3 years) healthy controls (HC 70.4 5.4 were compared. The cfDNA concentrations lengths measured using electrophoresis platform, indices (NLR, PLR, LMR, SII) calculated complete blood cell analysis. More fragmented 4-fold higher 50-700 bp detected than controls. group was significantly longer (median 336 bp) HC 271 bp). Optimal threshold 1265 pg/μL (AUC = 0.857) 290 0.814). A Kaplan-Meier survival curves analysis also demonstrated a mortality risk with cut-off >303 cfDNA. is first have revealed association high levels > 1000 length, which can be aggravated by immunoinflammatory reactivity.

Язык: Английский

Процитировано

1
Endothelial Dysfunction with Aging: Does Sex Matter? DOI Open Access

Jakub Jozue Wojtacha,

Barbara Morawin, Edyta Wawrzyniak-Gramacka

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(22), С. 12203 - 12203

Опубликована: Ноя. 13, 2024

Oxidative stress and inflammation accompany endothelial dysfunction that results from the excessive or uncontrolled production of reactive oxygen nitrogen species (RONS) in older adults. This study was designed to assess usefulness serum oxi-inflammatory component combinations vascular disease prediction prevention with regard sex. Women (n = 145) men 50) aged 72.2 ± 7.8 years participated this project. The females demonstrated elevated hydrogen peroxide (H2O2) nitric oxide (NO) responsible for intravascular low-density lipoprotein oxidation. NO generation enhanced women, but its bioavailability reduced, which expressed by a high 3-nitrotyrosine (3-NitroT) concentration. relation NO/3-NitroT (rs 0.811, p < 0.001) women −0.611, showed sex determines dysfunction. RONS simultaneously promoted regeneration, as ~1.5-fold increase circulating progenitor cells. Inflammation-specific variables, such neutrophil-to-lymphocyte ratio, systemic immune index, neutrophil-to-high-density (HDL) were reduced their diagnostic utility clinical prognosis dysfunction, especially C-reactive-protein-to-HDL ratio (AUC 0.980, specificity 94.7%, sensitivity 93.3%, OR 252, 95% CI 65–967, 0.001). is first have revealed sex-specific changes response, can generate risk cardiovascular events at an age.

Язык: Английский

Процитировано

1