PP2A Affects Angiogenesis via Its Interaction with a Novel Phosphorylation Site of TSP1
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(3), С. 1844 - 1844
Опубликована: Фев. 3, 2024
Alterations
in
angiogenic
properties
play
a
pivotal
role
the
manifestation
and
onset
of
various
pathologies,
including
vascular
diseases
cancer.
Thrombospondin-1
(TSP1)
protein
is
one
master
regulators
angiogenesis.
This
study
unveils
novel
aspect
TSP1
regulation
through
reversible
phosphorylation.
The
silencing
B55α
regulatory
subunit
phosphatase
2A
(PP2A)
endothelial
cells
led
to
significant
decrease
expression.
Direct
interaction
between
PP2A-B55α
was
confirmed
via
methods.
Truncated
constructs
were
employed
identify
phosphorylation
site
responsible
kinase,
ultimately
pinpointing
PKC
as
enzyme
phosphorylating
on
Ser93.
biological
effects
B55α–TSP1
also
analyzed.
not
only
counteracted
increase
expression
during
wound
closure
but
prolonged
time.
Although
silenced
initiated
tube-like
structures
earlier
than
control
cells,
their
spheroid
formation
disrupted,
leading
disintegration.
Cells
transfected
with
phosphomimic
S93D
exhibited
smaller
spheroids
reduced
effectiveness
tube
formation,
revealing
insights
into
properties.
In
this
paper,
we
introduce
new
mechanism
angiogenesis
by
S93
PP2A
B55α.
Язык: Английский
Large Language Model‐Assisted Genotoxic Metal‐Phenolic Nanoplatform for Osteosarcoma Therapy
Small,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 13, 2024
Abstract
Osteosarcoma,
a
leading
primary
bone
malignancy
in
children
and
adolescents,
is
associated
with
poor
prognosis
low
global
fertility
rate.
A
large
language
model‐assisted
phenolic
network
(LLMPN)
platform
demonstrated
that
integrates
the
model
(LLM)
GPT‐4
into
design
of
multifunctional
metal‐phenolic
materials.
Fine‐tuned
identified
gossypol
as
compound
superior
efficacy
against
osteosarcoma
after
evaluating
across
library
60
polyphenols
based
on
correlation
between
experimental
anti‐osteosarcoma
activity
multiplexed
chemical
properties
polyphenols.
Subsequently,
then
self‐assembled
Cu
2+
‐gossypol
nanocomplexes
hyaluronic
acid
surface
modification
(CuGOS
NPs).
CuGOS
NPs
has
ability
to
induce
genetic
alterations
cell
death
cells,
offering
significant
therapeutic
benefits
for
tumors
reducing
metastasis
without
adverse
effects
major
organs
or
genital
system.
This
work
presents
an
LLM‐driven
approach
engineering
metal‐organic
nanoplatform
broadening
applications
by
harnessing
capabilities
LLMs,
thereby
improving
feasibility
efficiency
research
activities.
Язык: Английский