Unveiling the potential of dendrobine: insights into bioproduction, bioactivities, safety, circular economy, and future prospects

Critical Reviews in Biotechnology, Год журнала: 2025, Номер unknown, С. 1 - 19

Опубликована: Янв. 6, 2025

This comprehensive review aims to explore and consolidate the current knowledge on dendrobine, including its biological activities, molecular mechanisms of action, effects various physiological processes, potential toxicity, safety considerations, in order unlock full applications. Dendrobine has diverse effects, anti-inflammatory, antioxidant, neuroprotective, immunomodulatory, anticancer effects. also exerts neuroprotective by boosting neuronal survival, reducing neuroinflammation, regulating neurotransmitter release. immunomodulatory that control immune cell function modify immunological responses. exhibits properties decreasing tumor angiogenesis metastasis, causing apoptosis cancer cells, inhibiting growth. discusses idea a circular economy produce researching cutting-edge sustainable methods maximize resource efficiency reduce environmental Additionally, supporting principles for production outlining future study areas, this discovery will help use dendrobine variety applications while preserving environment. Future research should focus advancing application personalized medicine, exploring green bioproduction methods, ensuring ethical sourcing practices. An enhanced understanding synergistic therapies could revolutionize healthcare, whereas conservation efforts safeguard ecological impact.

Язык: Английский

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Recent Advances and New Insights in Genome Analysis and Transcriptomic Approaches to Reveal Enzymes Associated with the Biosynthesis of Dendrobine-Type Sesquiterpenoid Alkaloids (DTSAs) from the Last Decade

Molecules, Год журнала: 2024, Номер 29(16), С. 3787 - 3787

Опубликована: Авг. 10, 2024

Dendrobium species, which are perennial herbs widely distributed in tropical and subtropical regions, notable for their therapeutic properties attributed to various bioactive compounds, including dendrobine-type sesquiterpenoid alkaloids (DTSAs). The objective of this review article is provide a comprehensive overview recent advances the biosynthesis DTSAs, extraction from species endophytes, elucidation associated genes through genomic transcriptomic sequencing both spp. exploration biosynthetic pathways drawing conclusions outlining future perspectives field. Alkaloids, predominantly nitrogen-containing compounds found medicinal orchids, include over 140 types discovered across more than 50 species. identified 37 picrotoxane alkaloids, have distinctive five-membered nitrogen heterocyclic ring. This highlights endophytic fungi as alternative sources emphasizing potential pharmaceutical applications when plant-derived scarce or complex. Genomic endophytes has key involved DTSAs biosynthesis, elucidating such mevalonate (MVA) 2-C-methyl-D-erythritol 4-phosphate (MEP) pathways. Genes encoding enzymes, acetyl-CoA C-acetyltransferase diphosphomevalonate decarboxylase, positively with dendrobine production. Despite significant advancements, complexity terpenoid different subcellular compartments remains challenge. Future research should focus on leveraging high-quality data omics technologies further understand manipulate enhance use.

Язык: Английский

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Characterization of main degradation products from dendrobine under stress conditions by multistage cleavage of UPLC-ESI-IT-TOF

Journal of Pharmaceutical and Biomedical Analysis, Год журнала: 2025, Номер 255, С. 116663 - 116663

Опубликована: Янв. 5, 2025

Язык: Английский

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Unveiling the rhizosphere microbiome of Dendrobium: mechanisms, microbial interactions, and implications for sustainable agriculture

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Янв. 29, 2025

The rhizosphere microbiome plays a critical role in plant health and productivity by fostering beneficial microbial interactions that support nutrient cycling, stress tolerance, disease suppression. In the context of Dendrobium , understanding its is essential for optimizing cultivation promoting sustainable agricultural practices. This review explores focusing on mechanisms contribute to health, growth their implications agriculture. study highlights diverse composition communities rhizosphere, including key bacteria (e.g., Pseudomonas fluorescens Bacillus subtilis ), fungi Glomus spp.), biocontrol agents ( Trichoderma discusses roles suppression, promotion. emphasizes significance plant-microbe signaling, such as production flavonoids, phytohormones, strigolactones, shaping environment enhancing resilience. Additionally, it addresses modern techniques analyzing communities, metagenomics next-generation sequencing, applications advancing precision Future research should focus bridging knowledge gaps related genotype-microbiome interactions, exploring emerging consortia integration management agriculture systems improve productivity.

Язык: Английский

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Dynamic Changes in Functional Components of Dendrobium officinale and Their Applications in Food Science: A Review

Plant Foods for Human Nutrition, Год журнала: 2025, Номер 80(1)

Опубликована: Фев. 10, 2025

Язык: Английский

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Enhancement of Dendrobine Production by CRISPR/Act3.0-Mediated Transcriptional Activation of Multiple Endogenous Genes in Dendrobium Plants

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1487 - 1487

Опубликована: Фев. 11, 2025

Dendrobine, a significant medicinal compound, typically accumulates at low concentrations within several Dendrobium species, including nobile, catenatum, and moniliforme. As D. nobile catenatum are established ingredients in traditional Chinese medicine have been cultivated extensively, they present ideal plant chassis for upscaling dendrobine production industrial research applications. This study employed two approaches: the ectopic overexpression of seven genes through multigene stacking activation multiple key endogenous synthesis pathway using CRISPR/Act3.0 either or catenatum. These methods enhanced transiently infiltrated leaves by 30.1% transgenic plants 35.6%. The is first to apply orchids, enhancing production, thus laying solid foundation further improvements. recently developed technique that demonstrates high efficiency model rice, maize, Arabidopsis. By combining CRISPR with transcriptional regulatory modules, metabolic can be achieved. successful application orchid molecular breeding reveals promising potential exploration.

Язык: Английский

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Metabolic and Transcriptomic Profile Revealing the Differential Accumulating Mechanism in Different Parts of Dendrobium nobile

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(10), С. 5356 - 5356

Опубликована: Май 14, 2024

Dendrobium nobile is an important orchid plant that has been used as a traditional herb for many years. For the further pharmaceutical development of this resource, combined transcriptome and metabolome analysis was performed in different parts D. nobile. First, saccharides, organic acids, amino acids their derivatives, alkaloids were main substances identified Amino derivatives flavonoids accumulated strongly flowers; saccharides phenols flowers fruits; leaves nucleotide its leaves, flowers, fruits. Simultaneously, genes lipid metabolism, terpenoid biosynthesis, alkaloid biosynthesis highly expressed phenylpropanoids flavonoid roots; other metabolisms leaves. Furthermore, members metabolic enzyme families like cytochrome P450 4-coumarate-coA ligase showed differential effects on tissue-specific accumulation. Members transcription factor AP2-EREBP, bHLH, NAC, MADS, MYB participated widely ATP-binding cassette transporters some also positive These results systematically elucidated molecular mechanism accumulation enriched library specialized products promising candidate genes.

Язык: Английский

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Association Analysis of METTL23 Gene Polymorphisms with Reproductive Traits in Kele Pigs

Genes, Год журнала: 2024, Номер 15(8), С. 1061 - 1061

Опубликована: Авг. 12, 2024

Methyltransferase-like 23 (METTL23) is a kind of RNA methyltransferase that catalyzes the methylation transfer to N6-adenosine RNA, serving as one key mediators in this process. However, METTL23 gene has been poorly researched pigs. In study, we investigated genetic effects single-nucleotide polymorphism(SNPs) on reproductive traits Kele The DNA was extracted from 228 healthy multiparous sows, and Sanger sequencing revealed three SNPs, g.4804958 G > T (intron 2), g.4805082 C (exon g.4806821 A 3). polymorphism information content (PIC) for each SNP 0.264, 0.25, 0.354, indicating moderate (0.25 < PIC 0.5) providing information. Linkage disequilibrium analysis showed no strong linkage between SNPs. association individuals with GG genotype had significantly higher number piglets born alive, litter birth weight, weaned piglets, weaning weight compared those TT (p 0.05). Individuals group average than AA H4H4 diplotype significant Together, could be used candidate selection

Язык: Английский

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Exploring the medicinal potential of Dendrobium: Uncovering the spatial distribution of flavonoids and alkaloids in 15 species of Dendrobium using MALDI-MSI

Scientia Horticulturae, Год журнала: 2024, Номер 338, С. 113738 - 113738

Опубликована: Окт. 20, 2024

Язык: Английский

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Characterization of an aminotransferase TlBCAT from Trichoderma longibrachiatum UN32 involved in dendrobine-type total alkaloids biosynthesis

World Journal of Microbiology and Biotechnology, Год журнала: 2024, Номер 40(12)

Опубликована: Ноя. 9, 2024

Язык: Английский

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