Distinct Ventral Hippocampus Network Properties in Dissociated Cultures DOI Creative Commons
Menahem Segal

Hippocampus, Год журнала: 2024, Номер 34(12), С. 744 - 752

Опубликована: Ноя. 2, 2024

Extensive research has been focused in the past century on structural, physiological, and molecular attributes of hippocampus. This interest was created by unique involvement hippocampus cognitive affective functions brain. Functional analysis revealed that divergent properties along its axial dimension to extent dorsal sector (dorsal hippocampus, DH) different connections with rest brain than those ventral (VH). Still, longitudinal pathways connect DH VH dampen functional differences between sectors. To be able identify intrinsic difference VH, we produced dissociated monolayer cultures from prenatal examined their at 10-20 days after plating imaging spontaneous activity network using Fluo-2 AM, a calcium indicator. Surprisingly, while sectors similar morphological attributes, were more active spontaneously cultures. Furthermore, when stimulated produce action potentials, neurons triggered bursts postsynaptic often Finally, both cultures, electrical stimulation single cells response burst potentials rather spikes. These experiments indicate even are excitable sensitive DH; hence, they likely generate epileptic seizures, as suggested for vivo brains.

Язык: Английский

Distinct Ventral Hippocampus Network Properties in Dissociated Cultures DOI Creative Commons
Menahem Segal

Hippocampus, Год журнала: 2024, Номер 34(12), С. 744 - 752

Опубликована: Ноя. 2, 2024

Extensive research has been focused in the past century on structural, physiological, and molecular attributes of hippocampus. This interest was created by unique involvement hippocampus cognitive affective functions brain. Functional analysis revealed that divergent properties along its axial dimension to extent dorsal sector (dorsal hippocampus, DH) different connections with rest brain than those ventral (VH). Still, longitudinal pathways connect DH VH dampen functional differences between sectors. To be able identify intrinsic difference VH, we produced dissociated monolayer cultures from prenatal examined their at 10-20 days after plating imaging spontaneous activity network using Fluo-2 AM, a calcium indicator. Surprisingly, while sectors similar morphological attributes, were more active spontaneously cultures. Furthermore, when stimulated produce action potentials, neurons triggered bursts postsynaptic often Finally, both cultures, electrical stimulation single cells response burst potentials rather spikes. These experiments indicate even are excitable sensitive DH; hence, they likely generate epileptic seizures, as suggested for vivo brains.

Язык: Английский

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