A genome-wide CRISPR/Cas9 knockout screen identifies SEMA3F gene for resistance to cyclin-dependent kinase 4 and 6 inhibitors in breast cancer

Breast Cancer, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

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Developing pioneering pharmacological strategies with CRISPR/Cas9 library screening to overcome cancer drug resistance

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Год журнала: 2024, Номер 1879(6), С. 189212 - 189212

Опубликована: Ноя. 1, 2024

Язык: Английский

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Identification of host factors for livestock and poultry viruses: genome-wide screening technology based on the CRISPR system

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 21, 2024

Genome-wide CRISPR library screening technology is a gene function research tool developed based on the CRISPR/Cas9 gene-editing system. The clustered regularly interspaced short palindromic repeats/CRISPR-associated genes (CRISPR/Cas) system, considered third generation of editing after zinc finger nucleases (ZFN) and transcription activator-like effector (TALEN), widely used for various viral host factors. libraries are classified into three main categories different functions Cas9 enzymes: knockout (CRISPR KO) screening, transcriptional activation (CRISPRa) interference (CRISPRi) screening. Recently, genome-wide has been to identify factors that interact with viruses at stages, including adsorption, endocytosis, replication. By specifically modulating expression these factors, it becomes possible cultivate disease-resistant varieties, establish disease models, design develop vaccines, among other applications. This review provides an overview development technical processes as well its applications in identifying livestock poultry.

Язык: Английский

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The application of CRISPR/Cas9–based genome-wide screening to disease research

Molecular and Cellular Probes, Год журнала: 2024, Номер 79, С. 102004 - 102004

Опубликована: Дек. 21, 2024

Язык: Английский

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