Molecular Mechanism of Exogenous ABA to Enhance UV-B Resistance in Rhododendron chrysanthum Pall. by Modulating Flavonoid Accumulation

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(10), С. 5248 - 5248

Опубликована: Май 11, 2024

With the depletion of ozone layer, intensity ultraviolet B (UV-B) radiation reaching Earth's surface increases, which in turn causes significant stress to plants and affects all aspects plant growth development. The aim this study was investigate mechanism response UV-B endemic species Rhododendron chrysanthum Pall. (R. chrysanthum) Changbai Mountains how exogenous ABA regulates R. stress. results chlorophyll fluorescence images OJIP kinetic curves showed that damaged PSII photosystem chrysanthum, could alleviate damage some extent. A total 2148 metabolites were detected by metabolomics, flavonoids accounted for highest number (487, or 22.67%). KEGG enrichment analysis differential accumulation revealed flavonoid biosynthesis flavone flavonol significantly altered. GO most DEGs produced after distributed cellular process, anatomical entity, catalytic activity. Network key DFs associated with synthesis identified (isorhamnetin-3-O-gallate dihydromyricetin) genes (TRINITY_DN2213_c0_g1_i4-A1) promote resistance In addition, multiple transcription factor families found be involved regulation pathway under Overall, actively responded regulating changes flavonoids, especially flavones flavonols, while further enhanced its experimental not only provide a new perspective understanding molecular but also valuable theoretical basis future research application improving adversity tolerance.

Язык: Английский

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Calcium signaling regulates the accumulation of phenolic acids in response to UV-B stress in Rhododendron chrysanthum Pall.

Plant Cell Reports, Год журнала: 2024, Номер 43(9)

Опубликована: Авг. 31, 2024

Язык: Английский

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WRKY Transcription Factors Modulate the Flavonoid Pathway of Rhododendron chrysanthum Pall. Under UV-B Stress

Plants, Год журнала: 2025, Номер 14(1), С. 133 - 133

Опубликована: Янв. 4, 2025

The depletion of the ozone layer has resulted in elevated ultraviolet-B (UV-B) radiation levels, posing a significant risk to terrestrial plant growth. Rhododendron chrysanthum Pall. (R. chrysanthum), adapted high-altitude and high-irradiation environments, developed unique adaptive mechanisms. This study exposed R. UV-B for two days, with an 8 h daily treatment, utilizing metabolomic transcriptomic analyses explore role WRKY transcription factors plant’s stress response their regulation flavonoid synthesis. decrease rETR Ik increase 1-qP. These chlorophyll fluorescence parameters indicate that impaired photosynthesis chrysanthum. Faced detrimental impact radiation, is capable mitigating its effects by modulating biosynthetic pathways adapt positively stress. revealed changes expression 113 flavonoid-related metabolites 42 associated genes, showing correlation these alterations. can influence key enzyme genes metabolic pathway, thereby affecting metabolite production. A theoretical reference investigating physiology provided this work, which also offers insights into responses alpine plants under adverse conditions.

Язык: Английский

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Role of syringic acid in enhancing growth, photosynthesis, and antioxidant defense in lettuce exposed to arsenic stress

Physiologia Plantarum, Год журнала: 2025, Номер 177(1)

Опубликована: Янв. 1, 2025

Heavy metal pollution, especially arsenic toxicity, significantly impairs plant growth and development. Phenolic acids, known for their antioxidant properties involvement in stress signaling, are gaining increased attention as secondary metabolites with the potential to enhance resistance these stressors. This study aimed investigate effects of different concentrations syringic acid (SA1, 10 μM; SA2, 250 SA3, 500 μM) on growth, photosynthetic parameters, activity lettuce seedlings subjected (As, 100 μM). Arsenic reduced by 56.7%, water content 7.39%, osmotic 26.2% leaves compared control. Conversely, SA1 SA2 treatments mitigated adverse preserved balance plants. However, SA3 treatment led a decrease 18.9% 39.5% As+SA3 groups, respectively, indicating that high-dose SA adversely affected under both control conditions. Exogenous improved photosynthesis, whereas provided milder benefits did not reduce exposure. H2O2 47.3% lipid peroxidation 33.4% seedlings. effectively oxidative enhancing activities key enzymes, such superoxide dismutase (SOD) peroxidase (POX). Moreover, was successful maintaining glutathione (GSH) pool, primarily promoted ascorbate (AsA) regeneration. In conclusion, μM (SA1) identified optimal dose reducing supporting growth. Overall, findings underscore resilience heavy toxicity.

Язык: Английский

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Integrated Metabolomic and Transcriptomic Analysis Reveals the Molecular Regulatory Mechanism of Gamma-Aminobutyric Acid Accumulation in White Quinoa (Chenopodium quinoa Willd.) in Response to Dark and Ultrasound Stress

Foods, Год журнала: 2025, Номер 14(7), С. 1186 - 1186

Опубликована: Март 28, 2025

Gamma-aminobutyric acid (GABA) is a nonprotein amino acid, which confers stress resistance to plants. Precise mechanisms underlying GABA accumulation in quinoa (Chenopodium quinoa) subjected dark and ultrasonic stresses have not been elucidated. We conducted transcriptome metabolome analyses of samples exposed various treatments reveal molecular pathways leading accumulation. Through the comprehensive integration data, an association was revealed between accumulation, 9 differentially expressed metabolites, 27 genes. Two responsible for synthesis were identified, involving glutamate decarboxylase aldehyde dehydrogenases, respectively. These enzymes regulate enrichment under conditions. demonstrated that stress, proline alanine increased, whereas arginine declined. Phenolic flavonoids, alkaloid metabolites increased. findings provide novel insights into mechanism by darkness ultrasound enhance GABA, supporting development targeted synthetic biology techniques.

Язык: Английский

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Golden 2-like Transcription Factors Regulate Photosynthesis under UV-B Stress by Regulating the Calvin Cycle

Plants, Год журнала: 2024, Номер 13(13), С. 1856 - 1856

Опубликована: Июль 5, 2024

UV-B stress can affect plant growth at different levels, and although there is a multitude of evidence confirming the effects radiation on photosynthesis, are fewer studies using physiological assays in combination with multi-omics to investigate photosynthesis alpine plants under stressful environments. Golden 2-like (G2-like/GLK) transcription factors (TFs) highly conserved during evolution may be associated abiotic stress. In this paper, we used Handy-PEA Imaging-PAM Maxi detect chlorophyll fluorescence leaves Rhododendron chrysanthum Pall. (R. chrysanthum) after stress, also investigated effect abscisic acid (ABA) We proteomics, widely targeted metabolomics, transcriptomics study changes photosynthesis-related substances The results showed that was able impair donor side photosystem II (PSII), inhibit electron transfer weaken alleviate damage caused by photosynthetic apparatus. Significant G2-like occurred R. differentially expressed genes localized Calvin cycle were strongly correlated members TF family. Multi-omics measurements together revealed TFs influence regulating cycle. This paper provides insights into conducive adoption measures improve increase yield.

Язык: Английский

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RcTRP5 Transcription Factor Mediates the Molecular Mechanism of Lignin Biosynthesis Regulation in R. chrysanthum against UV-B Stress

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(17), С. 9205 - 9205

Опубликована: Авг. 24, 2024

UV-B stress destroys the photosynthetic system of Rhododendron chrysanthum Pall. (R. chrysanthum), as manifested by decrease efficiency and membrane fluidity, also promotes accumulation lignin. The MYB (v-myb avian myeloblastosis viral oncogene homolog) family transcription factors can be involved in response to through regulation lignin biosynthesis. This study indicated that both donor recipient sides R. were significantly damaged based on physiological index measurements made using OJIP curves under stress. analysis bioinformatics data revealed RcTRP5 factor exhibits upregulation acetylation at K68 site, directly regulating biosynthesis Additionally, there was K43 site downregulation K83 CAD enzyme, well K391 PAL enzyme. Based these findings, we conjectured facilitates modification enzymes, thereby indirectly influencing demonstrated alleviate damage caused chrysanthum, which provides relevant ideas for improving content plants, a reference metabolic mechanism other secondary substances.

Язык: Английский

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UV-B Stress-Triggered Amino Acid Reprogramming and ABA-Mediated Hormonal Crosstalk in Rhododendron chrysanthum Pall.

Plants, Год журнала: 2024, Номер 13(16), С. 2232 - 2232

Опубликована: Авг. 12, 2024

Increased UV-B radiation due to ozone depletion adversely affects plants. This study focused on the metabolite dynamics of Rhododendron chrysanthum Pall. (R. chrysanthum) and role ABA in mitigating stress. Chlorophyll fluorescence metrics indicated that both JA increased resistance; however, effect was not as strong ABA. Metabolomic analysis using UPLC−MS/MS (ultra-performance liquid chromatography tandem mass spectrometry) revealed significant fluctuations metabolites under application. decreased amino acids phenolics, suggesting antioxidant defense activation. treatment upregulated lipids phenolic acids, highlighting its protective role. Multivariate showed distinct metabolic clusters pathways responding ABA, which impacted acid metabolism hormone signal transduction. Exogenous negatively regulated signaling pathway UV-B-exposed R. chrysanthum, shown by KEGG enrichment. deepens understanding plant stress-tolerance mechanisms has implications for enhancing stress tolerance through hormonal interventions.

Язык: Английский

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Transcriptomic and Metabolomic Analyses Reveal the Response Mechanism of Ophiopogon japonicus to Waterlogging Stress

Biology, Год журнала: 2024, Номер 13(3), С. 197 - 197

Опубликована: Март 20, 2024

Ophiopogon japonicus, a plant that thrives in river alluvial dams, often faces waterlogging stress due to sustained rainfall and flood seasons, which significantly impacts its growth development. Currently, the mechanisms of tolerance japonicus are still unclear. This study analyzed transcriptome metabolome data for Sichuan region (referred as CMD) under varying degrees stress: mild, moderate, severe. The results indicate group exposed flooding exhibited higher number differentially expressed genes (DEGs) compared control group. Notably, most DEGs were downregulated primarily enriched phenylpropanoid biosynthesis, starch sucrose metabolism, hormone signal transduction pathways. A total 5151 accumulated metabolites (DAMs) identified, with upregulated DAMs annotated two clusters, namely flavonoids such apiin, pelargonin, others. Furthermore, our revealed significant upregulation expression C2H2 (C2H2 zinc finger proteins) AP2/ERF-ERF (the subfamily ERF proteins APETALA2/ethylene-responsive element binding factors) transcription factors CMD stress, suggesting their critical roles enabling adapt these conditions. In conclusion, this research provides insights into intricate molecular underlying CMD’s response reports valuable genetic development transgenic plants improved tolerance.

Язык: Английский

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Strigolactone modulates phenolic acid accumulation and thereby improves tolerance to UV-B stress in Rhododendron chrysanthum Pall.

Plant Cell Reports, Год журнала: 2024, Номер 44(1)

Опубликована: Дек. 6, 2024

Язык: Английский

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