Prospective Assessment of VI-RADS with Muscle Invasion in Urinary Bladder Cancer and Its Implication on Re-Resection/Restaging TURBT Patients

Annals of Surgical Oncology, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 4, 2024

Язык: Английский

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Correlation between TCF7 and bladder cancer and feasibility of Erlotinib targeting in bladder cancer: Molecular mechanism and expression of TCF7 recombinant protein

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140438 - 140438

Опубликована: Янв. 1, 2025

Язык: Английский

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DLEU2 facilitates bladder cancer progression through miR-103a-2-5p/SOS1 axis

PeerJ, Год журнала: 2025, Номер 13, С. e18995 - e18995

Опубликована: Апрель 8, 2025

Bladder cancer (BC) represents a life-threatening malignancy within the urinary system. Dysregulated long non-coding RNAs (lncRNAs) play pivotal roles in advancement of BC. LncRNA deleted lymphocytic leukemia 2 (DLEU2) is implicated development various cancers. However, its role and regulatory mechanisms BC remain unclear. This research aimed to explore expression, biological function, molecular DLEU2 In progression. Expression profiles lncRNAs, microRNAs (miRNAs), mRNAs normal tissues were examined by leveraging raw data sourced from NCBI GEO database. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) validated expression levels cells. To evaluate proliferation migration capabilities cells, assays such as CCK-8, EdU, Transwell, scratch carried out. Luciferase reporter interactions between miR-103a-2-5p with SOS1. Protein SOS1 cells was analyzed via western blotting. markedly increased tissues. Functionally, overexpression elevated cell migration, while knockdown produced opposite effects. Mechanistically, acted sponge for miR-103a-2-5p, which targeted enhanced co-knockdown reversed these Overexpression also promoted counteracted overexpression. Conversely, inhibited processes, reversing this inhibition. These findings demonstrate that facilitates progression miR-103a-2-5p/SOS1 axis. study reveals novel mechanism underlying highlights potential therapeutic target treatment.

Язык: Английский

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The Role of Immunohistochemistry as a Surrogate Marker in Molecular Subtyping and Classification of Bladder Cancer

Diagnostics, Год журнала: 2024, Номер 14(22), С. 2501 - 2501

Опубликована: Ноя. 8, 2024

Bladder cancer (BC) is a highly heterogeneous disease, presenting clinical challenges, particularly in predicting patient outcomes and selecting effective treatments. Molecular subtyping has emerged as an essential tool for understanding the biological diversity of BC; however, its implementation practice remains limited due to high costs complexity genomic techniques. This review examines role immunohistochemistry (IHC) surrogate marker molecular BC, highlighting potential bridge gap between advanced classifications routine application; Methods: We explore evolution taxonomic classification with particular focus on cytokeratin (KRT) expression patterns normal urothelium, which are key identifying basal luminal subtypes. Furthermore, we emphasise need consensus IHC markers reliably define these subtypes, facilitating wider standardised use. The also analyses application both muscle-invasive (MIBC) non-muscle-invasive bladder (NMIBC), attention less extensively studied NMIBC cases. discuss practical advantages subtyping, including cost effectiveness feasibility standard pathology laboratories, alongside ongoing challenges such requirement protocols external validation across diverse settings; Conclusions: While limitations, it offers viable alternative laboratories lacking access Further research required determine optimal combination markers, establish diagnostic algorithm, validate through large-scale trials. will ultimately enhance accuracy, guide treatment decisions, improve outcomes.

Язык: Английский

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Exploring Darunavir, Rilpivirine and Etravirine as Potential Therapies for Bladder Cancer: Efficacy and Synergistic Effects

Biomedicines, Год журнала: 2024, Номер 12(3), С. 647 - 647

Опубликована: Март 14, 2024

This research explores the therapeutic efficacy of Darunavir (DRV), Rilpivirine (RPV), and Etravirine (ETV) against UM-UC-5 bladder cancer cells, addressing critical need for innovative treatments in research. Through a comprehensive assessment their individual combined effects across diverse time intervals, ETV emerges as most potent drug, with lowest IC50 5.9 µM, closely followed by RPV (lowest 9.6 µM), while DRV exhibits least effectiveness 25.6 µM). Notably, significant synergistic effect is evident combination, especially at 48 72 h low concentrations. Synergies are also observed DRV, albeit to lesser extent primarily h. Conversely, combination yields minimal effects, predominantly additive nature. In summary, this pre-clinical investigation underscores promising potential RPV, both standalone hinting repurposing opportunities therapy, which could give new treatment method disease that faster without severe side anticancer drugs. These findings represent substantial stride advancing personalized medicine within will be further scrutinized forthcoming studies.

Язык: Английский

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Psychological Impact of Bladder Cancer: Insights from 219 Patients and Caregivers in Indonesia Using DASS-21 (2019-2023)

Medical Science Monitor, Год журнала: 2024, Номер 30

Опубликована: Июль 5, 2024

BACKGROUND Bladder cancer (BC) is a common disease worldwide. Low survival rates and high recurrence lead to the risk of mental disorders. This study analyzed 219 patients with bladder using Indonesian Version 21-item Depression, Anxiety, Stress Scale (DASS-21) related factors. MATERIAL AND METHODS cross-sectional included diagnosed during 2019-2023 in referral hospital Indonesia. Data were collected questionnaire that assessed characteristics DASS-21 questionnaire. RESULTS Some caregivers experienced depression, stress, anxiety. The percentage who anxiety was higher than for (47.9% vs 45.7%). Statistical analysis showed age, income, marital status associated incidence depression (p=0.000, p=0.001, p=0.000, respectively), p=0.012, stress p=0.007, respectively). Routes patient admission (p=0.043, respectively) employment (p=0.005, Employment also (p=0.038, found routes among (p=0.036 CONCLUSIONS Monitoring providing support are needed prevent poor prognosis due psychological problems, including paying attention sociodemographic

Язык: Английский

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CONTENT OF DOPAMINE, NORADRENALINE, SEROTONIN AND 5-HYDROXYINDOLACETIC ACID IN THE URINE OF PATIENTS WITH BLADDER CANCER DEPENDING ON THE PRESENCE OF DIABETES MELLITUS

Современные проблемы науки и образования (Modern Problems of Science and Education), Год журнала: 2024, Номер №4 2024, С. 22 - 22

Опубликована: Янв. 1, 2024

СОДЕРЖАНИЕ ДОФАМИНА, НОРАДРЕНАЛИНА, СЕРОТОНИНА И 5-ОКСИИНДОЛУКСУСНОЙ КИСЛОТЫ В МОЧЕ БОЛЬНЫХ РАКОМ МОЧЕВОГО ПУЗЫРЯ ЗАВИСИМОСТИ ОТ НАЛИЧИЯ САХАРНОГО ДИАБЕТАГорошинская И.А

Язык: Русский

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ASO Author Reflections: Reevaluating Muscle Invasion in Bladder Cancer: The Role of VI-RADS in Guiding Re-resections/ Re-stage TURBT

Annals of Surgical Oncology, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 11, 2024

Язык: Английский

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Use of 3′ Rapid Amplification of cDNA Ends (3′ RACE)-Based Targeted RNA Sequencing for Profiling of Druggable Genetic Alterations in Urothelial Carcinomas

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(22), С. 12126 - 12126

Опубликована: Ноя. 12, 2024

Targeted treatment of advanced or metastatic urothelial carcinomas (UCs) requires the identification druggable mutations. This study describes development a 3' Rapid Amplification cDNA Ends (3' RACE)-based targeted RNA sequencing panel which accounts for status all genes relevant to UC treatment, namely,

Язык: Английский

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Heterogeneity and risk prediction study of self-reported outcomes in patients with abdominal wall stoma for bladder cancer based on latent profile analysis and machine learning algorithms

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Дек. 5, 2024

Abdominal wall stomas in bladder cancer patients can severely impact physical, psychological, and social adjustment. However, individual differences patients' self-reported outcomes their influencing factors require further investigation. This multicenter, cross-sectional study aimed to assess the health status of with abdominal stomas, identify heterogeneous characteristics among individuals, screen for risk affecting quality life. We evaluated 598 who underwent radical cystectomy an stoma between September 2020 2023 urology departments five tertiary hospitals across Henan Jiangxi Provinces, China. Data were collected via a self-designed general profile questionnaire Chinese Patient Self-Reported Outcome Scale Bladder Cancer Wall Stoma (PROS-BCAS). Latent analysis identified three patient subgroups: "balanced high quality" (n = 339, 56.7%), "unbalanced" 148, 24.7%), "low level" 111, 18.6%). Significant observed scores four PROS-BCAS domains these categories (p < 0.05). The associated "low-level" group included age (45–60 years), low income (monthly per capita <$2,000), self-payment healthcare, total lack self-care ability, presence comorbidities. XGBoost machine learning model key prognosis, such as monthly income, age, healthcare payment method, care status, comorbidities, descending order importance. These findings emphasize significant heterogeneity stomas. Clinical caregivers should therefore tailor interventions improve life, particularly vulnerable subgroups this study.

Язык: Английский

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