Brain Sciences,
Год журнала:
2024,
Номер
14(12), С. 1192 - 1192
Опубликована: Ноя. 26, 2024
Background:
Glioblastoma
(GBM)
is
the
most
common
and
deadly
type
of
brain
cancer
in
adults.
Dysregulation
receptor
tyrosine
kinase
pathways,
such
as
epidermal
growth
factor
(EGFR),
contributes
to
therapeutic
resistance.
Drugs
that
inhibit
activity
monoclonal
antibodies
against
EGFR
are
strategies
used
clinical
trials.
Photodynamic
therapy
(PDT)
a
tumor
treatment
involves
administration
photosensitizing
drug,
followed
by
its
activation
with
visible
light,
which
causes
cell
death
due
oxidative
stress.
Although
PDT
helps
prolong
median
survival
patients
GBM,
complete
remission
has
not
been
achieved.
Populations
GBM
cells
have
obtained
from
T98G
line
resistant
methyl-5-aminolevulinic
acid
(Me-ALA)
for
characterization,
comparing
them
original
parental
population.
Objective:
The
objective
this
work
was
evaluate
general
response
when
inhibited
drug
erlotinib.
Methods
Results:
It
observed
inhibitor
combination
reduced
viability
(MTT)
populations
compared
alone.
Furthermore,
PpIX
content
(flow
cytometry)
increased
population
were
incubated
Me-ALA
Erlotinib
prevented
proliferation
spheroids.
Wound
closure
both
PDT-resistant
populations.
Conclusions:
Our
results
indicate
would
be
relevant
resistance
PDT.
It
is
well
known
how
the
precise
localization
of
glioblastoma
multiforme
(GBM)
predicts
direction
tumor
spread
in
surrounding
neuronal
structures.
The
aim
present
review
to
reveal
lateralization
GBM
by
evaluating
anatomical
regions
where
it
frequently
located
as
main
molecular
alterations
observed
different
brain
regions.
According
literature,
or
most
frequent
has
yet
be
determined.
However,
can
said
that
more
frontal
lobe.
Tractus
and
fascicles
involved
appear
focused
on
corticospinal
tract,
superior
longitudinal
I,
II
III
fascicles,
arcuate
fascicle
long
segment,
strait
inferior
fronto‑occipital
fasciculus.
Considering
features
its
involvement,
logical
are
frontal‑temporal‑parietal‑occipital
lobes,
respectively.
Although
volumes
higher
right
hemisphere,
been
determined
prognosis
patients
diagnosed
with
cancer
left
hemisphere
worse,
probably
reflecting
distribution
some
detrimental
such
TP53
mutations,
PTEN
loss,
EGFR
amplification,
MGMT
promoter
methylation.
There
theories
stating
less
exposed
external
influences
development
responsible
for
functions
necessary
survival
while
tumors
may
aggressive.
To
shed
light
specific
regions,
article
aimed
at
describing
pathways
gene
mutations
epigenetic
modifications
associated
tumors.
Current Issues in Molecular Biology,
Год журнала:
2024,
Номер
46(9), С. 9881 - 9894
Опубликована: Сен. 5, 2024
The
race
to
find
an
effective
treatment
for
glioblastoma
(GBM)
remains
a
critical
topic,
because
of
its
high
aggressivity
and
impact
on
survival
the
quality
life.
Currently,
due
GBM's
heterogeneity,
conventional
success
rate
response
therapy
are
relatively
low,
with
median
less
than
20
months.
A
new
point
view
can
be
provided
by
comprehension
tumor
microenvironment
(TME)
in
pursuance
development
therapeutic
strategies
aim
longer
improved
life
disease-free
interval
(DFI).
main
components
GBM
TME
represented
extracellular
matrix
(ECM),
glioma
cells
stem
(GSCs),
immune
(microglia,
macrophages,
neutrophils,
lymphocytes),
neuronal
cells,
all
them
having
dynamic
interactions
being
able
influence
tumoral
growth,
progression,
drug
resistance
thus
potential
target.
This
paper
will
review
latest
research
targets
form
up-to-date
strategy.
Frontiers in Oncology,
Год журнала:
2025,
Номер
14
Опубликована: Янв. 9, 2025
Gliomas,
particularly
glioblastomas
(GBM),
are
highly
aggressive
with
a
poor
prognosis
and
low
survival
rate.
Currently,
deoxyelephantopin
(DET)
has
shown
promising
anti-inflammatory
anti-tumor
effects.
Using
clinical
prognostic
analysis,
molecular
docking,
network
pharmacology,
this
study
aims
to
explore
the
primary
targets
signaling
pathways
identify
novel
GBM
treatment
approaches.
PharmMapper,
chemical
structure
of
DET
was
examined
for
possible
after
being
acquired
from
PubChem.
GBM-related
were
obtained
through
multi-omics
A
protein-protein
interaction
(PPI)
constructed
using
Cytoscape
STRING,
target
binding
evaluated
docking.
Enrichment
analysis
conducted
Metascape.
The
effects
on
cell
invasion,
apoptosis,
proliferation
assessed
in
vitro
assays,
including
Transwell,
EDU,
CCK8,
flow
cytometry.
Western
blot
performed
examine
components
PI3K/AKT
pathway.
Among
sixty-four
shared
identified,
JUN
CCND1
most
frequently
observed.
demonstrated
that
influenced
MAPK
pathways.
In
Transwell
significantly
inhibited
invasive
behavior
glioma
cells.
further
confirmed
downregulation
EGFR,
JUN,
PI3K/AKT.
inhibits
proliferation,
apoptosis
via
modulating
pathway,
highlighting
its
potential
as
therapeutic
strategy
treatment.
Molecules,
Год журнала:
2025,
Номер
30(5), С. 976 - 976
Опубликована: Фев. 20, 2025
Circulating
tumor
cells
(CTCs)
are
pivotal
in
cancer
metastasis
and
serve
as
valuable
biomarkers
for
diagnosis,
prognosis,
treatment
monitoring.
Traditional
CTC
capture
methods
predominantly
utilize
the
epithelial
cell
adhesion
molecule
(EpCAM)
a
marker
isolation.
However,
heterogeneity
of
these
circulating
epithelial-to-mesenchymal
transition
process
(wherein
acquire
mesenchymal
characteristics)
limit
efficacy
EpCAM-based
techniques.
In
this
paper,
we
critically
review
role
EpCAM
capture,
explore
impact
on
expression,
discuss
alternative
strategies
to
enhance
By
evaluating
limitations
EpCAM-mediated
challenges
posed
by
transition,
aim
provide
insights
into
development
more
comprehensive
liquid
biopsy
approaches
management.
