Extracellular Vesicles Derived from Opuntia ficus-indica Fruit (OFI-EVs) Speed Up the Normal Wound Healing Processes by Modulating Cellular Responses

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(13), С. 7103 - 7103

Опубликована: Июнь 28, 2024

Plant-derived extracellular vesicles (EVs) have been recognized as important mediators of intercellular communication able to transfer active biomolecules across the plant and animal kingdoms. EVs demonstrated an impressive array biological activities, displaying preventive therapeutic potential in mitigating various pathological processes. Indeed, simplicity delivering exogenous endogenous bioactive molecules mammalian cells with their low cytotoxicity makes suitable agents for new strategies a variety pathologies. In this study, were isolated from Opuntia ficus-indica fruit (OFI-EVs) characterized by particle size distribution, concentration, molecule composition. OFI-EVs had no obvious toxicity protective role inflammatory process oxidative stress vitro model chronic skin wounds. The results that pretreatment decreased activity gene expression pro-inflammatory cytokines (IL-6, IL-8, TNF-α) LPS-stimulated human leukemia monocytic cell line (THP-1). Furthermore, promote migration dermal fibroblasts (HDFs), speeding up normal wound healing This study sheds light, first time, on modulating processes such inflammation oxidation, thereby identifying candidates cutaneous

Язык: Английский

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New Nanovesicles from Prickly Pear Fruit Juice: A Resource with Antioxidant, Anti-Inflammatory, and Nutrigenomic Properties

Cells, Год журнала: 2024, Номер 13(21), С. 1756 - 1756

Опубликована: Окт. 23, 2024

Plant-derived nanovesicles represent a novel approach in the field of plant-derived biomaterials, offering sustainable and biocompatible option for various biomedical applications. The unique properties these vesicles, such as their ability to encapsulate bioactive compounds, make them suitable therapeutic, cosmetic, nutraceutical purposes. In this study, we have, first time, successfully bio-fabricated vesicles derived from Opuntia ficus-indica (FicoVes) using an efficient cost-effective method. Characterized by size approximately 114 nm negative zeta potential -20.9 mV, FicoVes exhibited excellent biocompatibility hemocompatibility, showing no reduction viability human animal cells. Our results showed that possess significant antioxidant they reduced ROS generation TBH-stimulated displayed anti-inflammatory reducing expression pro-inflammatory cytokines (Il 1β, TNF α) enhancing (IL4, IL10) following inflammatory stimulus. Furthermore, accelerated epithelial wound closure L929 fibroblast monolayers dose-dependent manner, highlighting role tissue repair. This study establishes promising candidate nutrigenomic applications, particularly context inflammation-related disorders healing. Further research, including vivo studies, is essential validate findings fully explore therapeutic potential.

Язык: Английский

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Plant-Derived Exosome-Like Nanovesicles in Chronic Wound Healing

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 11293 - 11303

Опубликована: Ноя. 1, 2024

Abstract: The incidence of chronic wounds is steadily increasing each year, yet conventional treatments for yield unsatisfactory results. delayed healing significantly affects patient quality life, placing a heavy burden on patients, their families, and the healthcare system. Therefore, there an urgent need to find new treatment methods wounds. Plant-derived exosome-like nanovesicles (PELNs) may be able accelerate wound healing. PELNs possess advantages such as good accessibility (due in part high isolation yields), low immunogenicity, stability. Currently, are limited reports regarding role associated mechanisms, highlighting novelty necessity further research. This review aims provide overview PELNs, discussing methods, composition, mechanisms action Finally, we summarize future opportunities challenges related use wounds, offer some insights solutions. Keywords: exosomes, nanovesicles, plants,

Язык: Английский

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Physical, biochemical, and biological characterization of olive-derived lipid nanovesicles for drug delivery applications

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Ноя. 18, 2024

Язык: Английский

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Desmodesmus pannonicus Water Extract Inhibits Melanin Synthesis and Promotes Wound Healing

Life, Год журнала: 2024, Номер 14(12), С. 1542 - 1542

Опубликована: Ноя. 25, 2024

This study investigated the multifaceted benefits of

Язык: Английский

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