Genome-wide identification and comparative analysis of the AP2/ERF gene family in Prunus dulcis and Prunus tenella: expression of PdAP2/ERF genes under freezing stress during dormancy

BMC Genomics, Год журнала: 2025, Номер 26(1)

Опубликована: Янв. 31, 2025

The AP2/ERF (APETALA2/ethylene responsive factor) transcription factor family, one of the largest in plants, plays a crucial role regulating various biological processes, including plant growth and development, hormone signaling, stress response. This study identified 114 116 genes genomes 'Wanfeng' almond (Prunus dulcis) 'Yumin' wild dwarf tenella), respectively. These were categorized into five subfamilies: AP2, DREB, ERF, RAV, Soloist. PdAP2/ERF PtAP2/ERF members both demonstrated high conservation protein motifs gene structures. Members families unevenly distributed across eight chromosomes, with 30 27 pairs segmental duplications 15 18 tandem repeated genes, promoter regions family contained numerous important cis-elements related to regulation, Expression pattern analysis revealed that exhibited characteristics under freezing at different temperatures perennial dormant branches. Quantitative fluorescence indicated might be more intensely expressed phloem branches almond, opposite trend observed xylem. compared initially explored expression patterns xylem findings provide theoretical foundation for future research on improvement breeding, as well molecular mechanisms underlying resistance stress.

Язык: Английский

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Comprehensive genomic screening and expression profiling of trihelix family in pearl millet under abiotic stresses with emphasis on functional insights of PgTHX24

Current Plant Biology, Год журнала: 2024, Номер 39, С. 100371 - 100371

Опубликована: Июль 6, 2024

The trihelix transcription factors (THX TFs) play a crucial role in light responses and are involved plant growth, development, stress responses. In this study, we have identified 35 TFs pearl millet (Pennisetum glaucum), which is one of the most widely grown C4 cereal crops tropical semi-arid regions. Identified PgTHXs (Trihelix members P. glaucum) were classified into 5 subgroups (GT1, GT2, GTγ, SH4, SIP1) based on phylogenetic analysis, these subgroup shared similar gene structure motif distribution pattern. Collinearity analysis exhibited duplication events family across genome. Gene ontology (GO) annotation cis-regulatory elements (CREs) suggested their involvement diverse biological molecular functions associated with RNA sequencing data expression profile displayed differential patterns under abiotic phytohormone treatments. induced pattern PgTHX4, PgTHX5, PgTHX24, PgTHX30 potential through phytohormonal signalling pathways. Among these, GT-3b member, was localized nucleus self-transactivation ability. Overexpression PgTHX24 positively regulated stress-related markers transformed calli drought conditions. Promoter activity also highlighted stress-inducible nature PgTHX24's promoter. Overall, our findings provide comprehensive understanding framework for further functional characterization to understand regulatory millet's Thirty-five millet, diversity. its

Язык: Английский

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Genome-wide identification and comprehensive analysis of the AP2/ERF gene family in Prunus sibirica under low-temperature stress

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Сен. 28, 2024

Язык: Английский

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Genome-wide identification and comprehensive analysis of the AP2/ERF gene family in Prunus sibirica under low-temperature stress

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июнь 6, 2024

Background AP2/ERF transcription factors (TFs) are plant-specific involved in the regulation of plant growth and development response to stresses. Although TFs have been identified numerous species analyzed many, such as Oryza sativa, Arabidopsis thaliana, and Populus trichocarpa, studies on Prunus sibirica (PsAP2/ERF) gene family not reported. In study, 112 PsAP2/ERFs were categorized into 16 subfamilies. Results The same subfamilies typically exhibited similar exon-intron structures motif compositions. all, 50 pairs segmentally duplicated genes within PsAP2/ERF family. results showed that 20 PsAP2/ERFswere highly expressed leaves, roots, pistils, under different conditions low temperature stress. Conclusions This study provides a solid scientific foundation for follow-up research evolutionary characteristics AP2/ERFs P. sibirica, leading an improved understanding molecular basis their developmental low-temperature stress responses, further investigating functional expression PsAP2/ERFs.

Язык: Английский

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Comprehensive Genome-Wide Identification and Characterization of the AP2 Subfamily in Beta vulgaris L. in Response to Exogenous Abscisic Acid

Agriculture, Год журнала: 2024, Номер 14(8), С. 1273 - 1273

Опубликована: Авг. 2, 2024

APETALA2 (AP2) belongs to transcription factor (TF) families, with crucial roles in regulating plant growth, development, and stress responses. In order explore the characteristics of sugar beet (Beta vulgaris L.) AP2s (BvAP2s) response drought hormone abscisic acid (ABA), genome-wide identification, phylogeny, gene structure promoter precursor analysis BvAP2s were employed predict their potential functions. It is shown that there are a total 13 BvAP2 genes Beta vulgaris. Based on primary amino sequence, can be further subdivided into euAP2, euANT basalANT. addition, cis-acting element showed contained several abiotic stress-related elements, including those associated ABA stress. Roots first perceive signals, ABA-treated beetroot transcriptome downstream prediction revealed BVRB_4g074790, BVRB_6g128480 BVRB_7g179610 may play an important role involved signaling pathways during by GRAM genes, LEAs U-boxes. Additionally, quantitative real-time polymerase chain reaction (qRT-PCR) confirmed downregulation these three induction roots. These findings provide basis for future utilization developing drought-tolerant varieties.

Язык: Английский

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Evolutionary progression and functional diversification of NAC family members in pearl millet with comprehensive characterization of PgNAC103 under drought stress

Plant Stress, Год журнала: 2024, Номер unknown, С. 100728 - 100728

Опубликована: Дек. 1, 2024

Язык: Английский

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Genome-wide identification, evolutionary expansion and expression divergence of <i>AP2/ERF</i> gene family in loquat (<i>Eriobotrya japonica</i>)

Fruit Research, Год журнала: 2020, Номер 0(0), С. 1 - 11

Опубликована: Янв. 1, 2020

Язык: Английский

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