LINC01270 Regulates the NF-κB-Mediated Pro-Inflammatory Response via the miR-326/LDOC1 Axis in THP-1 Cells DOI Creative Commons
Imene Arab, Su‐Geun Lim, Kyoungho Suk

и другие.

Cells, Год журнала: 2024, Номер 13(23), С. 2027 - 2027

Опубликована: Дек. 8, 2024

Long intergenic noncoding (LINC)01270 is a 2278 bp transcript belonging to the subset of long (lnc)RNAs. Despite increased reports LINC01270's involvement in different diseases, evident research on its effects inflammation yet be achieved. In present study, we investigated potential role LINC01270 modulating inflammatory response human monocytic leukemia cell line THP-1. Lipopolysaccharide treatment upregulated expression, and siRNA-mediated suppression enhanced NF-κB activity subsequent production cytokines IL-6, IL-8, MCP-1. Interestingly, knockdown downregulated expression leucine zipper cancer 1 (LDOC1), novel suppressor. An analysis LINC01270/micro-RNA (miRNA)/protein interactome profile identified miR-326 as possible mediator. Synthetic RNA agents that perturb interaction among LINC01270, miR-326, LDOC1 mRNA mitigated changes caused by THP-1 cells. Additionally, luciferase reporter assay HEK293 cells further confirmed enhances activation, while overexpression has opposite effect. This study provides insight into responses lipopolysaccharide stimulation via miR-326/LDOC1 axis, which negatively regulates activation.

Язык: Английский

LINC01270 Regulates the NF-κB-Mediated Pro-Inflammatory Response via the miR-326/LDOC1 Axis in THP-1 Cells DOI Creative Commons
Imene Arab, Su‐Geun Lim, Kyoungho Suk

и другие.

Cells, Год журнала: 2024, Номер 13(23), С. 2027 - 2027

Опубликована: Дек. 8, 2024

Long intergenic noncoding (LINC)01270 is a 2278 bp transcript belonging to the subset of long (lnc)RNAs. Despite increased reports LINC01270's involvement in different diseases, evident research on its effects inflammation yet be achieved. In present study, we investigated potential role LINC01270 modulating inflammatory response human monocytic leukemia cell line THP-1. Lipopolysaccharide treatment upregulated expression, and siRNA-mediated suppression enhanced NF-κB activity subsequent production cytokines IL-6, IL-8, MCP-1. Interestingly, knockdown downregulated expression leucine zipper cancer 1 (LDOC1), novel suppressor. An analysis LINC01270/micro-RNA (miRNA)/protein interactome profile identified miR-326 as possible mediator. Synthetic RNA agents that perturb interaction among LINC01270, miR-326, LDOC1 mRNA mitigated changes caused by THP-1 cells. Additionally, luciferase reporter assay HEK293 cells further confirmed enhances activation, while overexpression has opposite effect. This study provides insight into responses lipopolysaccharide stimulation via miR-326/LDOC1 axis, which negatively regulates activation.

Язык: Английский

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