Foxk2 Enhances Adipogenic Differentiation by Relying on the Transcriptional Activation of Peroxisome Proliferator‐Activated Receptor Gamma DOI Creative Commons
Shan Zhang, Yanru You, Ran Li

и другие.

Journal of Cellular and Molecular Medicine, Год журнала: 2025, Номер 29(1)

Опубликована: Янв. 1, 2025

ABSTRACT Proper differentiation of bone marrow stromal cells (BMSCs) into adipocytes is crucial for maintaining skeletal homeostasis. However, the underlying regulatory mechanisms remain incompletely understood, posing a challenge treatment age‐related osteopenia and osteoporosis. Here, through comprehensive gene expression analysis during BMSC adipocytes, we identified forkhead transcription factor Foxk2 as key regulator this process. was significantly higher in inguinal epididymal white adipose tissues db/db mice compared to non‐obese db/m controls induced BMSCs, C3H/10 T1/2, ST2 following adipogenic stimulation. Overexpression promoted ST2, accompanied by increased lipogenic factors. Conversely, silencing inhibited differentiation. Moreover, also facilitated lipogenesis T1/2 cells. Adipogenic stimuli triggered nuclear translocation PI3‐kinase mTOR signalling pathways. Once nucleus, directly bound promoters Pparγ1 Pparγ2, thereby enhancing their transcriptional activity. Notably, PPARγ1 PPARγ2 reciprocally augmented activity promoter, indicating presence Foxk2‐PPARγ positive feedback loop that drives adipogenesis.

Язык: Английский

Foxk2 Enhances Adipogenic Differentiation by Relying on the Transcriptional Activation of Peroxisome Proliferator‐Activated Receptor Gamma DOI Creative Commons
Shan Zhang, Yanru You, Ran Li

и другие.

Journal of Cellular and Molecular Medicine, Год журнала: 2025, Номер 29(1)

Опубликована: Янв. 1, 2025

ABSTRACT Proper differentiation of bone marrow stromal cells (BMSCs) into adipocytes is crucial for maintaining skeletal homeostasis. However, the underlying regulatory mechanisms remain incompletely understood, posing a challenge treatment age‐related osteopenia and osteoporosis. Here, through comprehensive gene expression analysis during BMSC adipocytes, we identified forkhead transcription factor Foxk2 as key regulator this process. was significantly higher in inguinal epididymal white adipose tissues db/db mice compared to non‐obese db/m controls induced BMSCs, C3H/10 T1/2, ST2 following adipogenic stimulation. Overexpression promoted ST2, accompanied by increased lipogenic factors. Conversely, silencing inhibited differentiation. Moreover, also facilitated lipogenesis T1/2 cells. Adipogenic stimuli triggered nuclear translocation PI3‐kinase mTOR signalling pathways. Once nucleus, directly bound promoters Pparγ1 Pparγ2, thereby enhancing their transcriptional activity. Notably, PPARγ1 PPARγ2 reciprocally augmented activity promoter, indicating presence Foxk2‐PPARγ positive feedback loop that drives adipogenesis.

Язык: Английский

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