bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Июль 7, 2023
Abstract
Extracellular
vesicles
(EVs),
membrane-delimited
nanovesicles
that
are
secreted
by
cells
into
the
extracellular
environment,
gaining
substantial
interest
due
to
their
involvement
in
cellular
homeostasis
and
contribution
disease
pathology.
The
latter
particular
has
led
an
exponential
increase
EVs
as
they
considered
be
circulating
packages
containing
potential
biomarkers
also
a
possible
biological
means
deliver
drugs
cell-specific
manner.
However,
several
challenges
hamper
straightforward
proteome
analysis
of
generally
low
abundant
reside
complex
matrices.
These
matrices
typically
contain
protein
concentrations
vastly
exceed
those
EV
proteome.
Therefore,
extensive
isolation
purification
protocols
imperative
many
have
been
developed,
including
(density)
ultracentrifugation,
size-exclusion
precipitation
methods.
Here,
we
describe
filter-aided
vesicle
enrichment
(FAEVEr)
approach
based
on
300
kDa
MWCO
filtration
allows
processing
multiple
samples
parallel
within
reasonable
timeframe
at
moderate
cost.
We
demonstrate
FAEVEr
is
capable
quantitatively
retaining
particles
filters,
whilst
allowing
washing
with
mild
detergent
TWEEN-20
remove
interfering
non-EV
proteins.
retained
directly
lysed
filter
for
complete
recovery
cargo
towards
analysis.
validate
optimize
recombinant
material
apply
it
conditioned
medium
well
serum.
Our
results
indicate
isolated
from
MCF7
cultured
or
without
serum
drastic
different
because
nutrient
deprivation.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(11), С. 5726 - 5726
Опубликована: Май 24, 2024
The
utility
of
serum
glial
fibrillary
acidic
protein
(GFAP)
in
acute
ischemic
stroke
(AIS)
has
been
extensively
studied
recent
years.
Here,
we
aimed
to
assess
its
potential
role
as
a
cargo
extracellular
vesicles
(EVs)
secreted
by
astrocytes
(ADEVs)
response
brain
ischemia.
Plasma
samples
from
eighteen
AIS
patients
at
24
h
(D1),
7
days
(D7),
and
one
month
(M1)
post-symptoms
onset,
nine
age,
sex,
cardiovascular
risk
factor-matched
healthy
controls
were
obtained
isolate
EVs
using
the
Exoquick
ULTRA
EV
kit.
Subsets
presumed
ADEVs
identified
further
expression
glutamate
aspartate
transporter
(GLAST)
specific
marker
with
Basic
Exo-Flow
Capture
Western
blotting
tested
presence
GFAP
ADEV
cargo.
Post-stroke
levels
elevated
D1
D7
but
not
M1
compared
(p
=
0.007,
p
0.019,
0.344,
respectively).
Significant
differences
highlighted
content
three
time
points
(n
12,
0.027)
between
(z
2.65,
0.023).
A
positive
correlation
was
observed
modified
Rankin
Scale
(mRS)
(r
0.58,
0.010)
0.57,
0.013),
respectively.
may
dynamically
reflect
changes
during
first
post-ischemia.
Profiling
peripheral
blood
could
provide
new
way
central
nervous
system
pathology.
Current Eye Research,
Год журнала:
2025,
Номер
unknown, С. 1 - 9
Опубликована: Фев. 12, 2025
The
cornea
is
a
transparent
avascular
tissue
that
serves
as
the
first
line
of
defense
against
opportunistic
pathogens
and
provides
smooth
refractive
surface
for
vision.
Due
to
its
external
location,
vulnerable
stress
from
outer
environment.
This
can
lead
corneal
epithelial
breakdown
subsequent
disease.
Extracellular
vesicles
(EVs)
are
nano-sized
enclosed
within
lipid
bilayer
secreted
by
all
cells
in
body
play
key
role
cell-to-cell
communication.
Within
field,
EVs
exosomes,
latter
which
represents
subpopulation
small
EVs,
have
emerged
potential
therapies
treating
diseases
increased
our
understanding
mechanisms
more
specifically,
exosomes
released
stressed
or
unhealthy
cells,
leads
dysfunction
We
conducted
literature
search
using
PubMed
Google
Scholar
keywords
relevant
extracellular
vesicles,
cornea.
reviewed
focusing
on
EV
studies
wound
healing
therapy.
review
comprehensive
overview
current
state
exosome
biology
it
relates
disease
healing.
Studies
date
provide
compelling
data
indicating
may
an
integral
maintenance
homeostasis.
also
exciting
therapeutics
disease;
their
presence
tear
fluid
serve
diagnostic
biomarkers
ocular
systemic
diseases.
While
still
infancy
state,
continued
progress
this
area
will
improve
functional
capacity
these
human
development
novel
regenerative
therapies.
Journal of Cosmetic Dermatology,
Год журнала:
2025,
Номер
24(4)
Опубликована: Март 27, 2025
ABSTRACT
Background
Acoustic
Wave
Therapy
(AWT)
is
a
particular
shock
wave
treatment
that
combines
focused
and
radial
waves,
resulting
in
efficiency
the
body's
superficial
layers.
Its
application
before
fat
grafting
has
potential
to
enhance
it,
favoring
better
result
of
graft.
Aims
The
aim
this
study
was
analyze
effects
AWT
on
tissue.
Methods
Histological
analysis
harvested
from
abdomen
40
subjects
evaluated.
Twenty
had
been
pre‐treated
with
AWT,
while
20
not.
Extraction
characterization
adipose‐derived
stem
cells
(ADSCs)
were
performed
for
all.
multilineage
differentiation
capacity
extracted
ADSCs
exosome
extracellular
vesicles'
production
samples
analyzed
both
groups
specimens.
Results
All
specimens
presented
preservation
structure
tissue
cells,
particularly
adipocytes.
cell
growth
resulted
higher
samples.
all
able
differentiate
along
adipogenic,
chondrogenic,
osteogenic
lineages.
In
particular,
samples,
adipogenic
lineage,
more
advanced
maturation
phase
observed,
chondrogenic
chondroid
matrix
evident
around
chondrocyte
aggregates.
reacted
stress.
Conclusions
results
highlighted
aligns
in‐force
minimal
manipulation
regulations,
as
cells'
functionality
preserved.
types
observed
permitted
us
speculate
about
possible
new
applications
aesthetic
regenerative
medicine.
Molecular Nutrition & Food Research,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 8, 2025
ABSTRACT
Bacterial
extracellular
vesicles
(BEVs)
have
emerged
as
versatile
and
promising
tools
for
therapeutic
interventions
across
a
spectrum
of
medical
applications.
Among
these,
Lactobacillus
‐derived
(LDEVs)
garnered
significant
attention
due
to
their
diverse
physiological
functions
applications
in
health
advancement.
These
LDEVs
modulate
host
cell
signaling
pathways
through
the
delivery
bioactive
molecules,
including
nucleic
acids
proteins.
The
immunomodulatory
properties
are
important,
they
been
shown
regulate
balance
between
pro‐inflammatory
anti‐inflammatory
responses
various
diseases.
play
crucial
role
maintaining
gut
homeostasis
by
modulating
composition
function
microbiota,
which
has
implications
conditions,
inflammatory
bowel
diseases,
metabolic
disorders,
neurological
disorders.
Furthermore,
hold
potential
deliver
payloads
specific
tissues
or
organs.
Engineered
can
be
loaded
with
agents
such
antimicrobial
peptides
acid‐based
therapies
treat
By
leveraging
unique
LDEVs,
researchers
develop
innovative
strategies
disease
prevention,
treatment,
overall
well‐being.
Thus,
this
review
aims
provide
comprehensive
overview
benefits
promoting
