Exploring the regulatory role of CNPY3 as a prognostic biomarker on human glioma cell migration, invasion and immune infiltration

Cancer Biomarkers, Год журнала: 2025, Номер 42(3)

Опубликована: Март 1, 2025

Background Canopy FGF signalling regulator 3 (CNPY3) is involved in immune regulation, tumorigenesis and development, nevertheless, its role glioma remains largely unexplored. Our study aimed to explore the regulatory of CNPY3 as a prognostic biomarker human cell migration, invasion infiltration. Methods Bioinformatics analysis clinical relevance public databases was performed. COX regression performed assess relationship between prognosis. GO Kyoto Encyclopedia Genes Genomes analyses were conducted predict signaling pathways gliomas. Tumor infiltration explored using TIMER, CIBERSORT, Pearson correlation analysis. GSVA single-cell sequencing data employed for further validation. The effects on migration cells investigated through scratch assay transwell assay. Results positively correlated with IDH mutation status, 1p/19q histopathologic grade, MGMT promoter methylation but negatively overall survival patients ( P < 0.05). significantly associated tumor response, inflammatory lipopolysaccharide-mediated pathway. influenced different types which affected microenvironment glioma. promoted increase M2 macrophage positive regulation macrophages apoptotic process. In vitro suggested promotion U87MG an increased capacity drug sensitivity showed more towards temozolomide, irinotecan, cisplatin among high expression Conclusion Increased impacts enhances recommended patients.

Язык: Английский

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Evaluation of Microvascular Density in Glioblastomas in Relation to p53 and Ki67 Immunoexpression

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(12), С. 6810 - 6810

Опубликована: Июнь 20, 2024

Glioblastoma is the most aggressive tumor in central nervous system, with a survival rate of less than 15 months despite multimodal therapy. Tumor recurrence frequently occurs after removal. Tumoral angiogenesis, formation neovessels, has positive impact on progression and invasion, although there are controversial results specialized literature regarding its survival. This study aims to correlate immunoexpression angiogenesis markers (CD34, CD105) proliferation index Ki67 p53 primary secondary glioblastomas. retrospective included 54 patients diagnosed glioblastoma at Pathology Department County Emergency Clinical Hospital Târgu Mureș. Microvascular density was determined using CD34 CD105 antibodies, were correlated p53, IDH1, ATRX Ki67. The number neoformed blood vessels varied among cases, characterized by different shapes calibers, endothelial cells showing modified morphology moderate marked pleomorphism. Neovessels glomeruloid aspect, associated intense positivity for or cells, observed, characteristic Mean microvascular values higher marker all though no statistically significant differences compared CD105. Mutant IDH1 glioblastomas, wild-type those above 20% showed more abundant density, statistical correlations not reaching significance. highlighted variety percentage intervals glioblastomas immunohistochemical CD105, respectively, correlation between evaluated

Язык: Английский

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Identification of ferroptosis-related LncRNAs as potential targets for improving immunotherapy in glioblastoma

Computer Methods in Biomechanics & Biomedical Engineering, Год журнала: 2025, Номер unknown, С. 1 - 13

Опубликована: Янв. 1, 2025

The effect of ferroptosis-related long non-coding RNAs (lncRNAs) in predicting immunotherapy response to glioblastoma (GBM) remains obscure. This study established a 11-lncRNAs prognostic signature. Differential gene expression analysis, univariate and multivariate Cox regression analyses the least absolute shrinkage selection operator (LASSO) algorithm were used identify genes establish nomogram model risk score. Kaplan-Meier survival plots receiver operating characteristic (ROC) curve analysis evaluate accuracy TCGA-GBM cohort. To verify these signatures, we analyzed levels three lncRNAs (AGAP2-AS1, OSMR-AS1, UNC5B-AS1) LN229 U87 cells. ROC showed that area under (AUC) this signature is 0.814, suggesting it has promising performance on GBM prediction. rate patients high-risk group was significantly lower than low-risk group, prediction superior conventional clinicopathological factors. Further qRT-PCR experiment also confirmed our lncRNA signatures. These might be therapeutic targets for glioblastoma, targeting can improve efficacy immunotherapy, especially immune checkpoint inhibitors. Mechanistically, findings attribute N6-methyladenosine (m6A) mRNA modification lncRNAs.

Язык: Английский

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Sesquiterpene Lactones as Promising Anti-Glioblastoma Drug Candidates Exerting Complex Effects on Glioblastoma Cell Viability and Proneural–Mesenchymal Transition

Biomedicines, Год журнала: 2025, Номер 13(1), С. 133 - 133

Опубликована: Янв. 8, 2025

Glioblastoma is one of the most aggressive brain cancers, characterized by active infiltrative growth and high resistance to radiotherapy chemotherapy. Sesquiterpene triterpenoids (STLs) their semi-synthetic analogs are considered as a promising source novel anti-tumor agents due low systemic toxicity multi-target pharmacological effects on key processes associated with tumor progression. The current review aims systematize knowledge anti-glioblastoma potential STLs accumulated over last decade identify in glioblastoma cells that susceptible action STLs. An analysis published data clearly demonstrated STLs, which can successfully cross blood-brain barrier, exert complex inhibitory effect through induction "mitochondrial dysfunction-oxidative stress-apoptosis" axis, inhibition glucose metabolism cell cycle phase transition, suppression motility invasion blockade proneural-mesenchymal transition. Taken together, this highlights not only able induce death, but also effectively affect diffusive spread, suggests possible directions for further investigation context better understand mechanism action.

Язык: Английский

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Boric acid impedes glioblastoma growth in a rat model: insights from multi-approach analysis

Medical Oncology, Год журнала: 2025, Номер 42(2)

Опубликована: Янв. 17, 2025

Abstract Limited advancements in managing malignant brain tumors have resulted poor prognoses for glioblastoma (GBM) patients. Standard treatment involves surgery, radiotherapy, and chemotherapy, which lack specificity damage healthy tissue. Boron-containing compounds, such as boric acid (BA), exhibit diverse biological effects, including anticancer properties. This study aimed to examine whether boron supplementation, BA, can inhibit growth a xenograft animal model. Using MRI-based tumor size measurement, survival rates, hematological, clinical biochemistry analyses, genotoxicity parameters, we assessed the impact of BA. Histopathological, immunohistochemical, immunofluorescence examinations were also conducted. All BA doses (3.25, 6.5, 13 mg kg −1 b.w.) extended compared GBM controls after 14 days, with dose-dependent anti-GBM effect observed MRI analyses. improved hematological (WBC PLT counts) biochemical parameters (LDL-C, CREA, ALP). Histopathological examination revealed significant reduction diameter 6.5 Immunohistochemical staining showed modulation intracytoplasmic Ki67, cytoplasmic CMPK2, GFAP expressions cells post-BA treatment. Additionally, did not increase micronuclei formations, indicating its non-genotoxic nature. In conclusion, targeting suppressor networks demonstrates therapeutic potential

Язык: Английский

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Advancing Glioblastoma Research with Innovative Brain Organoid-Based Models

Cells, Год журнала: 2025, Номер 14(4), С. 292 - 292

Опубликована: Фев. 16, 2025

Glioblastoma (GBM) is a relatively rare but highly aggressive form of brain cancer characterized by rapid growth, invasiveness, and resistance to standard therapies. Despite significant progress in understanding its molecular cellular mechanisms, GBM remains one the most challenging cancers treat due high heterogeneity complex tumor microenvironment. To address these obstacles, researchers have employed range models, including vitro cell cultures vivo animal often fail replicate complexity GBM. As result, there has been growing focus on refining models incorporating human-origin cells, along with advanced genetic techniques stem cell-based bioengineering approaches. In this context, variety based organoids were developed confirmed be clinically relevant are contributing advancement research at preclinical level. This review explores preparation use organoid-based deepen our biology explore novel therapeutic These innovative hold promise for improving ability study deadly advancing development more effective treatments.

Язык: Английский

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Advancing Glioblastoma Therapy: Learning From the Past and Innovations for the Future

Cancer Letters, Год журнала: 2025, Номер unknown, С. 217601 - 217601

Опубликована: Март 1, 2025

Язык: Английский

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Antineoplastic Activity of Methyl rosmarinate in Glioblastoma Cells

Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(3), С. 180 - 180

Опубликована: Март 10, 2025

Glioblastoma (GMB) is a remarkably aggressive brain malignancy characterized by high mortality rates, despite continuous advances in therapeutic approaches. Compounds derived from plants are being studied for their potent medicinal properties the quest more efficient therapies. This study investigated anti-glioma of Methyl rosmarinate, hydroxycinnamic acid isolated Thymus thracicus Velen, which has previously demonstrated anti-cancer activity various cell lines. Human glioblastoma lines U87 and T98 were treated with rosmarinate to assess its effect on viability, cycle distribution migratory capacity using Trypan blue assay, flow cytometry scratch wound healing respectively. The combinatorial effects temozolomide also analyzed CompoSyn software. According outcomes, significantly reduced induced death interfering checkpoints, inhibited migration both GMB Notably, cells, compound showed synergistic impact temozolomide, whereas there was an antagonistic relationship. These results suggest that potential properties; however, vivo research needed.

Язык: Английский

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Extracellular Vesicles for Disease Treatment

International Journal of Nanomedicine, Год журнала: 2025, Номер Volume 20, С. 3303 - 3337

Опубликована: Март 1, 2025

Traditional drug therapies suffer from problems such as easy degradation, side effects, and treatment resistance. disease diagnosis also suffers high error rates late diagnosis. Extracellular vesicles (EVs) are nanoscale spherical lipid bilayer secreted by cells that carry various biologically active components integral to intercellular communication. EVs can be found in different body fluids may reflect the state of parental cells, making them ideal noninvasive biomarkers for disease-specific The multifaceted characteristics render optimal candidates delivery vehicles, with evidence suggesting their efficacy ailments. However, poor stability degradation natural have affected applications. To solve EVs, they engineered modified obtain more stable multifunctional EVs. In this study, we review shortcomings traditional methods describe how modify form improve utilization. An innovative stimulus-responsive system has been proposed. We summarize current applications research status systemic diseases, look forward future directions, providing ideas scholars.

Язык: Английский

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Potential of Curcumin and Its Analogs in Glioblastoma Therapy

Antioxidants, Год журнала: 2025, Номер 14(3), С. 351 - 351

Опубликована: Март 18, 2025

Curcumin, a polyphenol found in turmeric, demonstrates multifaceted anti-cancer activity against glioblastoma. Its therapeutic potential stems from its ability to modulate various molecular pathways implicated glioblastoma development and progression, enhance the effectiveness of radiation therapy, induce cancer cell death through diverse mechanisms, including apoptosis, autophagy, cycle arrest. These combined actions make curcumin promising candidate for treatment, warranting further investigation into clinical application. In this review, we summarize latest research on analogs' therapy.

Язык: Английский

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