Stem Cell-Based Acellular Therapy: Insight into Biogenesis, Bioengineering and Therapeutic Applications of Exosomes

Biomolecules, Год журнала: 2024, Номер 14(7), С. 792 - 792

Опубликована: Июль 3, 2024

The vast regenerative potential of stem cells has laid the foundation for cell-based therapies. However, certain challenges limit application therapeutic use cell-free therapy can avoid limitations associated with Acellular therapies rely on biological factors released by cells, including growth and extracellular vesicles such as exosomes. Due to their comparable potential, acellular may provide a feasible scalable alternative Exosomes are small secreted various types cells. contain parent cell-derived nucleic acids, proteins, lipids, other bioactive molecules. They play an important role in intra-cellular communication influence characteristics inherit properties cells; therefore, exosomes particular interest applications medicine. In comparison therapy, exosome offers several benefits, easy transport storage, no risk immunological rejection, few ethical dilemmas. Unlike be lyophilized stored off-the-shelf, making standardized more accessible while reducing overall treatment costs. Exosome-based treatments therefore readily available patients at time care. current review discusses therapy. explores molecular mechanism biogenesis, methods isolation, characterization. addition, latest advancements bioengineering techniques enhance have been discussed. well diverse diagnosis diseases reviewed detail.

Язык: Английский

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Pharmacokinetic characteristics of mesenchymal stem cells in translational challenges

Signal Transduction and Targeted Therapy, Год журнала: 2024, Номер 9(1)

Опубликована: Сен. 13, 2024

Язык: Английский

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Stem Cell Therapy for Diseases of Livestock Animals: An In-Depth Review

Veterinary Sciences, Год журнала: 2025, Номер 12(1), С. 67 - 67

Опубликована: Янв. 17, 2025

Stem cells are unique, undifferentiated that have the ability to both replicate themselves and develop into specialized cell types. This dual capability makes them valuable in development of regenerative medicine. Current stem research has widened their application therapy, drug discovery, reproductive cloning animals, models for various diseases. Although there substantial studies revealing treatment human degenerative diseases using cells, this is yet be explored livestock animals. Many species such as mastitis, laminitis, neuromuscular disorders, autoimmune diseases, some debilitating not covered completely by existing drugs can improved different types like embryonic adult induced pluripotent cells. review mainly focuses on use disease In addition mentioned, potential helpful wound healing, skin genetic disorders. article explores from sources therapy also role conservation endangered well model preparation. Moreover, future perspectives challenges associated with discussed. Overall, transformative impact sector comprehensively studied which will help researchers design work related

Язык: Английский

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Towards Stem Cell Therapy for Critical-Sized Segmental Bone Defects: Current Trends and Challenges on the Path to Clinical Translation

Journal of Functional Biomaterials, Год журнала: 2024, Номер 15(6), С. 145 - 145

Опубликована: Май 27, 2024

The management and reconstruction of critical-sized segmental bone defects remain a major clinical challenge for orthopaedic clinicians surgeons. In particular, regenerative medicine approaches that involve incorporating stem cells within tissue engineering scaffolds have great promise fracture management. This narrative review focuses on the primary components engineering—stem cells, scaffolds, microenvironment, vascularisation—addressing current advances translational regulatory challenges in landscape cell therapy defects. To comprehensively explore this research area offer insights future treatment options surgery, we examined latest developments advancements engineering, focusing those relevance recent years. Finally, present forward-looking perspective using

Язык: Английский

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Mesenchymal stem cell–derived extracellular vesicles in joint diseases: Therapeutic effects and underlying mechanisms

Journal of Orthopaedic Translation, Год журнала: 2024, Номер 48, С. 53 - 69

Опубликована: Июль 27, 2024

Joint diseases greatly impact the daily lives and occupational functioning of patients globally. However, conventional treatments for joint have several limitations, such as unsatisfatory efficacy side effects, necessitating exploration more efficacious therapeutic strategies. Mesenchymal stem cell (MSC)-derived EVs (MSC-EVs) demonstrated high efficacyin tissue repair regeneration, with low immunogenicity tumorigenicity. Recent studies reported that EVs-based therapy has considerable effects against diseases, including osteoarthritis, tendon ligament injuries, femoral head osteonecrosis, rheumatoid arthritis. Herein, we review potential various types MSC-EVs in aforementioned summarise mechanisms underlying specific biological MSC-EVs, discuss future prospects basic research on MSC-EV-based modalities their clinical translation. In general, this provides an in-depth understanding well mechanisms, which may be beneficial to translation treatment. The translational article: cell-free can effectively promote regeneration repair. When used treat desirable preclinical research. This supplement further treatment its

Язык: Английский

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Needle Radiofrequency Combined with Topical Exosome Therapy for Moderate to Severe Acne

Life, Год журнала: 2025, Номер 15(2), С. 141 - 141

Опубликована: Янв. 21, 2025

Objective: This case series aims to evaluate the efficacy, patient satisfaction, and safety of combined needle radiofrequency (RF) topical exosome therapy for moderate severe acne. Methods: study involved 22 patients (12 females 10 males, ages 18–35) with acne who underwent RF (Xomage, Zishel Bio Inc., Seoul, Republic Korea) treatments. Each completed between 6 sessions, conducted weekly over three-week intervals. Acne severity was assessed using Investigator’s Global Assessment (IGA) scale, while satisfaction measured on a 5-point Likert scale. Clinical photographs were taken at baseline after final treatment session. Results: All showed improvement in mean decrease IGA score 2.5 points from assessment. Patient high, majority expressing skin texture reduction. Conclusion: Needle appears be an effective reducing lesions improving quality, demonstrating strong profile high satisfaction.

Язык: Английский

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Microneedling: monotherapy or adjuvant treatment?

Journal of Aesthetic Nursing, Год журнала: 2025, Номер 14(1), С. 38 - 42

Опубликована: Янв. 2, 2025

Microneedling, a minimally invasive procedure involving repeated puncturing of the skin with sterilized microneedles, has gained popularity for treating various dermatological concerns. Its ability to stimulate collagen production makes it effective rejuvenation, scar reduction, and hair regrowth. Recent studies demonstrate its enhanced efficacy when combined other treatments, such as minoxidil, platelet-rich plasma, exosomes regeneration. Additionally, microneedling shows promise in managing conditions like vitiligo melasma used alongside topical therapies. The expanding applications highlight versatility, combination therapies proving more than monotherapies optimal patient outcomes.

Язык: Английский

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Resveratrol-Enhanced Human Neural Stem Cell-Derived Exosomes Mitigate MPP+-Induced Neurotoxicity Through Activation of AMPK and Nrf2 Pathways and Inhibition of the NLRP3 Inflammasome in SH-SY5Y Cells

Life, Год журнала: 2025, Номер 15(2), С. 294 - 294

Опубликована: Фев. 13, 2025

Parkinson's disease (PD) is a progressive neurodegenerative disorder primarily characterized by the loss of dopaminergic neurons in substantia nigra. Mitochondrial dysfunction, oxidative stress, and neuroinflammation are recognized as critical pathological mechanisms driving neurodegeneration PD. Exosome (Exo)-based therapies, particularly those derived from human neural stem cells (hNSCs), offer promising neuroprotective effects due to their ability transfer bioactive molecules that modulate cellular processes. Resveratrol (RES), polyphenolic compound with potent antioxidant anti-inflammatory properties, has been shown enhance therapeutic potential cell (SC)-derived Exos. This study investigated RES-treated hNSCs-derived Exos (RES-hNSCs-Exos) on SH-SY5Y exposed 1-methyl-4-phenylpyridinium (MPP+), neurotoxin commonly used model Parkinsonian neurotoxicity. Treating MPP+ led significant reductions viability, mitochondrial increased activation inflammatory pathways. Treatment RES-hNSCs-Exos rescued MPP+-induced toxicity improving enhancing ATP production, increasing biogenesis, reducing reactive oxygen species (ROS) generation. The findings also demonstrated expression essential genes involved such PGC1α, NRF1, Tfam, indicating improved function presence RES-hNSCs-Exos. Further analysis revealed these protective were mediated activating AMP-activated protein kinase (AMPK) Nrf2 signaling pathways, which promoted health reduced stress. Moreover, treatment suppressed downregulating NLRP3 inflammasome secretion pro-inflammatory cytokines IL-1β IL-18. In conclusion, results suggest exhibit against neurotoxicity function, inhibiting neuroinflammation. These highlight hNSCs-Exos novel strategy for diseases like PD, RES valuable enhancer efficacy.

Язык: Английский

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Global research landscape on nanotechnology in acute lung injury: a bibliometric analysis

Frontiers in Digital Health, Год журнала: 2025, Номер 7

Опубликована: Март 4, 2025

Background Acute lung injury is a common respiratory emergency that seriously affects the life, health and quality of life patients, especially after global COVID-19 pneumonia. The application nanotechnology in acute promising. In response to knowledge explosion resulting from rapid publication growth, we applied bibliometric analysis explore research profile thematic trends field. Methods Articles reviews related 2004 2023 were searched. Java-based Citespace, VOSviewer, R software-based Bibiometrix used systematically evaluate publications by spatiotemporal distribution, author subject categories, topic references, keywords. Results A total 1,347 included. number papers has grown exponentially over past 20 years. China was most productive country out all 53 countries, followed United States. Chinese Academy Sciences institution with 76 papers. PARTICLE AND FIBRE TOXICOLOGY journal. top five high-frequency keywords inflammation, oxidative stress, toxicity, vitro , respiratory-distress-syndrome. And emerging delivery, covid-19, extracellular vesicles, therapy, sars-cov-2. Drug delivery are focus current research. Two areas represented development trends: novel nanocarriers higher efficiency lower biotoxicity, other impact nanomaterials progression injury. Conclusion field been period last three Delivery,targeted exosm have this trends:novel biotoxicity such as exosomes solid lipid nanoparticles,

Язык: Английский

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Comparison of Methods for Isolating Exosomes from Plasma Subjects with Normal and High Fat Percentages

Life, Год журнала: 2025, Номер 15(3), С. 410 - 410

Опубликована: Март 6, 2025

Adipose tissue is responsible for fat storage and an important producer of extracellular vesicles (EVs). The biological content exosomes, one kind EV, provides information on aspects such as immunometabolic alterations. This study aimed to compare three plasma exosome isolation methods—using a commercial kit (CK), size exclusion chromatography (SEC), differential centrifugation (DC)—and select the best one. Individuals categorized by normal high body percentages were used. DC CK proven be most advantageous out methods, so we suggest these methods further protein molecular analyses, respectively. Still, emphasize importance selecting appropriate methodology depending specific research objectives. At same time, no statistical differences in quality, morphology, total protein, or microRNA concentration observed between individuals percentage, that exosomal cargo varies with percentages.

Язык: Английский

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