Dipeptidyl peptidase-4 marks distinct subtypes of human adipose stromal/stem cells with different hepatocyte differentiation and immunoregulatory properties

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 8, 2024

Background Human adipose-derived stromal/stem cells (hASCs) play important roles in regenerative medicine and numerous inflammatory diseases. However, their cellular heterogeneity limits the effectiveness of treatment. Understanding distinct subtypes hASCs phenotypic implications will enable selection appropriate subpopulations for targeted approaches or Methods hASC expressing dipeptidyl peptidase-4 (DPP4) were identified via fluorescence-activated cell sorting (FACS) analysis. DPP4 expression was knocked down DPP4⁺ via siRNA. The capacity proliferation, hepatocyte differentiation, factor secretion T-cell functionality regulation from DPP4⁻, DPP4⁺, control siRNA-treated assessed. Results presented a lower proliferative but greater differentiation than DPP4⁻ hASCs. Both secreted high levels vascular endothelial growth factor-A (VEGF-A), monocyte chemoattractant protein-1 (MCP-1), interleukin 6 (IL-6), whereas other factors, including matrix metalloproteinase (MMP)-1, eotaxin-3, fractalkine (FKN, CX3CL1), growth-related oncogene-alpha (GRO-alpha, CXCL1), monokine induced by interferon-gamma (MIG), macrophage protein (MIP)-1beta, colony-stimulating (M-CSF), significantly supernatants those Exposure to conditioned media triggered changes cytokine profiles T healthy donors. percentage functional that factors such as MIP-1b IL-8 increased when these cocultured with polyfunctional CD8⁺ multiple IL-17A, tumour necrosis alpha (TNF-α) TNF-β, decreased derived Conclusions may regulate These data provide key foundation understanding role cells, which be helpful future immune activation studies allow them customized clinical application.

Язык: Английский

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The Identification of Key Genes and Biological Pathways in Cardiac Arrest by Integrated Bioinformatics and Next Generation Sequencing Data Analysis

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 19, 2024

Abstract Cardiac arrest (CA) is a common cause of death world wide. The disease has lacks effective treatment. Efforts have been made to elucidate the molecular pathogenesis CA, but mechanisms remain elusive. To identify key genes and pathways in next generation sequencing (NGS) GSE200117 dataset was downloaded from Gene Expression Omnibus (GEO) database. DESeq2 tool used recognize differentially expressed (DEGs). ontology (GO) REACTOME pathway enrichment analyses were performed analyze DEGs associated signal g:Profiler IID database construct protein-protein interaction (PPI) network, modules analysis using Cytoscape. A miRNA-hub gene regulatory network TF-hub then constructed screen miRNAs, TFs hub by miRNet NetworkAnalyst Cityscape software. Receiver operating characteristic curve (ROC) verified genes. In total, 844 identified, comprising 414 up regulated 430 down GO indicated that for CA mainly enriched organonitrogen compound metabolic process, response stimulus, translation immune system. Ten (up-regulated: HSPA8, HOXA1, INCA1 TP53; down-regulated: HSPB1, LMNA, SNCA, ADAMTSL4 PDLIM7) screened. We also predicted miRNAs (hsa-mir-1914-5p hsa-mir-598-3p) (JUN PRRX2) targeting This study uses series bioinformatics technologies obtain hug genes, TFs, related CA. These results provide us with new ideas finding biomarkers treatment methods

Язык: Английский

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Innate Immunity and Angiogenesis

Elsevier eBooks, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Язык: Английский

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Dipeptidyl peptidase-4 marks distinct subtypes of human adipose stromal/stem cells with different hepatocyte differentiation and immunoregulatory properties

Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)

Опубликована: Сен. 29, 2024

Язык: Английский

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The effect of hyperbaric oxygen therapy on oxidative stress and inflammation in patients with diabetic foot ulcers: A Preliminary Study

Heliyon, Год журнала: 2024, Номер 10(23), С. e40586 - e40586

Опубликована: Ноя. 20, 2024

Patients with an uncontrolled glycemic index develop a wide variety of pathologies associated diabetes, such as diabetic foot ulcers (DFUs). Hyperbaric oxygen therapy (HBOT) is adjunctive used to heal wounds and prevent lower extremity amputations in this population.

Язык: Английский

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Microglia regulate motor neuron plasticity via reciprocal fractalkine and adenosine signaling

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Ноя. 28, 2024

Язык: Английский

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