Transcriptomic and metabolomic analysis reveals the molecular mechanism of exogenous melatonin improves salt tolerance in eggplants

Frontiers in Plant Science, Год журнала: 2025, Номер 15

Опубликована: Янв. 10, 2025

Melatonin significantly enhances the tolerance of plants to biotic and abiotic stress, plays an important role in plant resistance salt stress. However, its molecular mechanisms eggplant stress have been rarely reported. In previous studies, we experimentally demonstrated that melatonin can enhance eggplants. this study, treated salt-stressed with a control treatment water, then conducted physiological biochemical tests, transcriptomic metabolomic sequencing, RT-qPCR validation at different stages after treatment. The results showed exogenous alleviate adverse effects on by increasing activity antioxidant enzymes, reducing content reactive oxygen species plants, organic osmoprotectants. Transcriptomic data, as well combined analysis, indicate activate metabolic pathways Compared genes α-linolenic acid metabolism pathway promote accumulation metabolites pathway, significant observed 48 hours treatment, activates expression such SmePLA2, SmeLOXs SmeOPR et al. α-Linolenic acid, (9R,13R)-12-oxophytodienoic 9(S)-HpOTrE (+)-7-iso-Jasmonic acid. validated activating effect candidate a-linolenic pathway. This study analyzed mechanism alleviating providing theoretical foundation for application enhancing production.

Язык: Английский

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Differential Expression of Amaranth AtrDODA Gene Family Members in Betalain Synthesis and Functional Analysis of AtrDODA1-1 Promoter

Plants, Год журнала: 2025, Номер 14(3), С. 454 - 454

Опубликована: Фев. 4, 2025

Betalains differ from anthocyanins, and they cannot coexist in the same plant under natural conditions. The L-DOPA 4,5-dioxygenase encoded by DODA gene is a key step pathway of betalain biosynthesis Caryophyllales plants. Amaranth an important resource for study extraction betalains. In order to clarify function AtrDODA family genes biosynthesis, we screened out three members associated with betalains based on genome database RNA-seq databases Amaranthus tricolor. Their characterization expression pattern were further analyzed. result subcellular localization showed that all located nucleus. Betacyanin betaxanthin promoted paclobutrazol treatment leaves stems ‘Suxian No.1’ (red), while inhibited gibberellin darkness, which consistent AtrDODAs. After heterologous transformation AtrDODA1-1 promoter into tobacco GUS staining analysis, activity (red) amaranth was significantly higher than No.2’ (green) amaranth. Furthermore, analyzed qRT-PCR after sprayed exogenous MeJA GA3 transformed results responded hormones. This could lay foundation revealing biological functions family, provide new clues molecular mechanism synthesis.

Язык: Английский

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Feasibility of Nano-Urea and PGPR on Salt Stress Amelioration in Reshmi Amaranth (Amaranthus tricolor): Stress Markers and Enzymatic Response

Horticulturae, Год журнала: 2025, Номер 11(3), С. 280 - 280

Опубликована: Март 5, 2025

The present experiment aimed to examine the impact of nano urea (NU) and plant growth-promoting rhizobacteria (PGPR) on Reshmi amaranth (Amaranthus tricolor) growth under salt stress. Experiments were conducted using six different combinations NaCl, NU, PGPR for 35 days greenhouse conditions. results showed that salinity stress significantly (p < 0.05) reduced parameters, including shoot height, root length, fresh weight, leaf area. However, application NU PGPR, both individually in combination, enhanced physiological resilience saline + treatment yielded best improvements, with a height 42.25 cm, length 34.79 weight 61.69 g, indicating synergistic effect. Biochemical analysis NaCl lowered chlorophyll (0.25 mg/g fwt.) carotenoids (60.17 µg/100 g) disrupted ionic homeostasis by increasing Na⁺ accumulation while reducing K+ Ca2+ uptake. combined restored balance, 58.12 mg K⁺ levels 115.25 78.70 mg, respectively. Stress markers such as malondialdehyde (MDA) proline also significant reductions, antioxidant enzyme activities stabilized application. Thus, this study indicated mitigate salt-induced improving nutrient assimilation, promoting osmotic regulation, enhancing antioxidative defenses amaranth.

Язык: Английский

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Deeper insights into the physiological and metabolic functions of the pigments in plants and their applications: beyond natural colorants

Physiologia Plantarum, Год журнала: 2025, Номер 177(2)

Опубликована: Март 1, 2025

Abstract Plant pigments are the natural source of color perceived by human senses. They have captivated researchers to explore their structural, physical, and chemical properties, synthesis mode, physiological significance. secondary metabolites in plants metabolism, growth regulation, photosynthesis, defense. Chlorophylls, carotenoids, anthocyanins, betalains major classes colors contributing shades textiles, foods, cosmetics. The bioactive properties these compounds used apply as pharmaceuticals treat chronic degenerative diseases like hypertension, diabetes, cancer, cardiovascular disorders. This review discusses classification, biosynthesis, metabolic activities, commercial applications plant pigments, knowledge gaps pigment identification application prospects. developments technologies such non‐encapsulation preserve unstable extracts for use transporter genes involved synthesizing essential pigments. However, additional research is required gain insight into candidate gene orchestrating stress responses potential engineering tolerance various crops.

Язык: Английский

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Dynamic transcriptomics and physiological insights reveal multi-tissue salt adaptation mechanisms in Amaranthus hypochondriacus across stress gradients

Plant Cell Reports, Год журнала: 2025, Номер 44(5)

Опубликована: Май 1, 2025

Язык: Английский

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Effects of liquid organic fertilizer on growth and volatile components of Arugula under salinity

BioResources, Год журнала: 2024, Номер 19(4), С. 7250 - 7278

Опубликована: Авг. 19, 2024

The effects of liquid organic fertilizer (LOF) made from anaerobic digestion on some physiological factors and volatile components arugula plants that were grown in salty conditions investigated. experiment was conducted three different stages. In the first two stages, seeds petri dishes pots for seven days. third stage involved growing seedlings 60 Salinity inhibited germination 7-day their emergence pots. these LOF pretreatment failed to eliminate stress-induced inhibition. Some parameters analyzed 60-day stage. Salt-induced inhibition showed significant negative all parameters. contrast previous (1% 5%) NPK had positive groups at stage, eliminating salt stress except water content. Contrary expectations, no change but fluctuating values due or treatments.

Язык: Английский

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Rhizophagus intraradices symbiosis with Amaranthus hypochondriacus improves rhizosphere soil pH and nutrient status in sodic soil

Rhizosphere, Год журнала: 2024, Номер unknown, С. 100973 - 100973

Опубликована: Окт. 1, 2024

Язык: Английский

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