Euterpe oleracea Mart. Bioactive Molecules: Promising Agents to Modulate the NLRP3 Inflammasome DOI Creative Commons
Carolina Bordin Davidson, Dana El Soufi El Sabbagh, A. K. Machado

и другие.

Biology, Год журнала: 2024, Номер 13(9), С. 729 - 729

Опубликована: Сен. 17, 2024

Inflammation is a vital mechanism that defends the organism against infections and restores homeostasis. However, when inflammation becomes uncontrolled, it leads to chronic inflammation. The NLRP3 inflammasome crucial in inflammatory responses has become focal point research for new anti-inflammatory therapies. Flavonoids like catechin, apigenin, epicatechin are known their bioactive properties (antioxidant, anti-inflammatory, etc.), but mechanisms behind actions remain unclear. This study aimed explore ability of various flavonoids (isolated combined) modulate using silico vitro models. Computer simulations, such as molecular docking, dynamics, MM/GBSA calculations examined interactions between molecules PYD. THP1 cells were treated with LPS + nigericin activate NLRP3, followed by flavonoid treatment at different concentrations. THP1-derived macrophages also following activation protocols. assays included colorimetric, fluorometric, microscopic, techniques. results showed had high binding affinity PYD, similar inhibitor MCC950. These flavonoids, particularly 1 µg/mL, 0.1 0.01 respectively, significantly reduced effects both cell types decreased pro-inflammatory cytokine, caspase-1, gene expression, suggesting potential agents through modulation.

Язык: Английский

Euterpe oleracea Mart. Bioactive Molecules: Promising Agents to Modulate the NLRP3 Inflammasome DOI Creative Commons
Carolina Bordin Davidson, Dana El Soufi El Sabbagh, A. K. Machado

и другие.

Biology, Год журнала: 2024, Номер 13(9), С. 729 - 729

Опубликована: Сен. 17, 2024

Inflammation is a vital mechanism that defends the organism against infections and restores homeostasis. However, when inflammation becomes uncontrolled, it leads to chronic inflammation. The NLRP3 inflammasome crucial in inflammatory responses has become focal point research for new anti-inflammatory therapies. Flavonoids like catechin, apigenin, epicatechin are known their bioactive properties (antioxidant, anti-inflammatory, etc.), but mechanisms behind actions remain unclear. This study aimed explore ability of various flavonoids (isolated combined) modulate using silico vitro models. Computer simulations, such as molecular docking, dynamics, MM/GBSA calculations examined interactions between molecules PYD. THP1 cells were treated with LPS + nigericin activate NLRP3, followed by flavonoid treatment at different concentrations. THP1-derived macrophages also following activation protocols. assays included colorimetric, fluorometric, microscopic, techniques. results showed had high binding affinity PYD, similar inhibitor MCC950. These flavonoids, particularly 1 µg/mL, 0.1 0.01 respectively, significantly reduced effects both cell types decreased pro-inflammatory cytokine, caspase-1, gene expression, suggesting potential agents through modulation.

Язык: Английский

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