Loss of aquaporin-4 impairs cerebrospinal fluid solute clearance through cerebrospinal fluid drainage pathways DOI Creative Commons
Daisuke Kato, Hiroyuki Kameda, Naoya Kinota

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 14, 2024

Abstract The aquaporin-4 (AQP4) water channel is essential in neurofluid dynamics. AQP4 loss impairs solute exchange between the cerebrospinal fluid (CSF) and interstitial (ISF). However, whether expression affects clearance from CSF space to extracranial remains unclear. This study aimed investigate this using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) following intrathecal administration of gadolinium-based contrast agents (GBCAs) knockout (KO) rats. KO rats showed reduced efflux GBCAs spaces through drainage pathways increased retention compared with controls. These results suggest that via pathways. revealed a close relationship clearance, contributing better understanding function

Язык: Английский

AQP4-specific T cells determine lesion localization in the CNS in a model of NMOSD DOI Creative Commons
Ali Maisam Afzali, Oleksii Ulianov, Lars Eckardt

и другие.

Acta Neuropathologica Communications, Год журнала: 2025, Номер 13(1)

Опубликована: Фев. 11, 2025

Abstract Neuromyelitis optica spectrum disorder (NMOSD) is a paradigmatic autoimmune disease of the central nervous system (CNS), in which water channel protein Aquaporin-4 (AQP4) targeted by self-reactive immune response. While immunopathology human NMOSD largely dependent on antibodies to astrocytic AQP4, role AQP4-specific T cells for localization and quality lesions CNS not known. Only recently, we established that thymic B express present AQP4 context MHC class II molecules purge naive cell receptor repertoire clones. Here, exploited this finding investigate lesion conditional AQP4-deficient ( Aqp4 ΔB ) mice, harbor precursors their can be sensitized mount strong AQP4(201–220)-specific CD4 + Sensitization mice with AQP4(201–220) was sufficient induce clinical disease. The spatiotemporal distribution glial response AQP4(201–220)-induced experimental encephalomyelitis (EAE) compared classical MOG(35–55)-induced EAE mice. In contrast MOG-EAE, characterized midline brain, retinal pathology, at grey matter/white matter border zone spinal cord. Therefore, conclude antigen-specific dictate NMOSD-lesions CNS.

Язык: Английский

Процитировано

1

Loss of aquaporin-4 impairs cerebrospinal fluid solute clearance through cerebrospinal fluid drainage pathways DOI Creative Commons
Daisuke Kato, Hiroyuki Kameda, Naoya Kinota

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 14, 2024

Abstract The aquaporin-4 (AQP4) water channel is essential in neurofluid dynamics. AQP4 loss impairs solute exchange between the cerebrospinal fluid (CSF) and interstitial (ISF). However, whether expression affects clearance from CSF space to extracranial remains unclear. This study aimed investigate this using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) following intrathecal administration of gadolinium-based contrast agents (GBCAs) knockout (KO) rats. KO rats showed reduced efflux GBCAs spaces through drainage pathways increased retention compared with controls. These results suggest that via pathways. revealed a close relationship clearance, contributing better understanding function

Язык: Английский

Процитировано

1