Huanglian Jiedu Tang regulates the inflammatory microenvironment to alleviate the progression of breast cancer by inhibiting the RhoA/ROCK pathway

Tissue and Cell, Год журнала: 2025, Номер 95, С. 102850 - 102850

Опубликована: Март 11, 2025

Язык: Английский

Sequencing of high-frequency mutated genes in breast cancer (BRCA) and associated-functions analysis

International Journal of Clinical and Experimental Pathology, Год журнала: 2025, Номер 18(2), С. 46 - 62

Опубликована: Янв. 1, 2025

Mutations or aberrant expression of genes in an organism tend not to be completely random and this cumulative effect predisposes the development malignant tumours. This study aims reveal possible high frequency mutated genes, then investigate their role development, prognosis, signalling pathway function drug resistance breast cancer. The cancer (BRCA) clinical samples were identified detected by high-throughput sequencing. High-frequency mutant counted. Gene profiles relationship with prognosis analysed throughout TCGA database. qRT-PCR was used analyse mRNA levels six high-frequency BRCA tissues cell lines. IHC protein tissues. linear interaction, single-cell layer clustering status influence immune infiltration degree among these bioinformatics analysis. STITCH cMAP datasets for gene interaction networks, association enrichment drug-transcriptome analyses. effects trastuzumab on proliferative capacity cells, as well determined CCK8 assay. that statistically found have mutations recruited present sequencing analysis included TP53, PIK3CA, NF1, TBX3, BRCA1 BRCA2. correlation further demonstrated using database: trend similar TCGA. showed BRCA2 higher tumor than normal samples, opposite a observed expressions displayed same IHC. Other results include 1) single resulted significant few overlapping regions; 2) different degrees infiltration; 3) between each other; 4) network had partially molecules; 5) PI3K key BRCA. Finally, proliferation ability confirmed optimal concentration its genes.

Язык: Английский

Eugenol inhibits NEAT1 as a ceRNA in pre-cancerous breast lesions

Heliyon, Год журнала: 2024, Номер 11(1), С. e41353 - e41353

Опубликована: Дек. 19, 2024

Язык: Английский