Overexpression of Tetrahymena Cysteine Synthetase 1 Promotes Cadmium Removal by Biosynthesizing Cadmium Sulfide Quantum Dots in Escherichia coli

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3685 - 3685

Опубликована: Апрель 13, 2025

Heavy metal cadmium causes significant contamination in aquatic ecosystems. The biomineralization of represents a vital biological mechanism for handling stress diverse microorganisms. To improve the capacity by microorganisms environments, Tetrahymena cysteine synthetase 1 (TtCsa1) was overexpressed E. coli. tolerance coli/pET-28a-TtCSA1 to enhanced expressing TtCsa1. Upon addition cysteine, generated more H2S, which reacted with Cd2+ form CdS quantum dots (QDs), resulting stronger fluorescence signal. UV-visible absorption and spectra culture supernatant showed characteristic peaks corresponding QDs. Transmission Electron Microscopy (TEM) images confirmed that formation QDs their agglomeration coli cells. X-ray Diffraction Analysis (XRD) analysis further presence crystalline nature. In rich medium, achieved removal rates 99.5%, 98.2%, 56.5%, 49.4%, respectively, concentrations 0.15, 0.3, 0.45, 0.6 mM within 48 h. simulated wastewater, 99.4%, 94.3%, 90.1%, 89.8%, 0.6, 0.75 12 These results demonstrate overexpressing TtCsa1 can significantly enhance its ability biomineralize medium has potential applications bioremediation environments contaminated heavy metals.

Язык: Английский

The Participation of Trehalose Metabolism in Response to High-Humidity Stress in Megoura crassicauda (Hemiptera: Aphididae)

Agronomy, Год журнала: 2024, Номер 14(12), С. 2958 - 2958

Опубликована: Дек. 12, 2024

In the context of climate change, characterized by an increase in average precipitation, agricultural pests have demonstrated enhanced adaptability to high humidity and other challenging environmental conditions, thereby intensifying need for effective prevention control measures. Among these pests, Megoura crassicauda (Hemiptera: Aphididae) represents a significant threat both crop yield quality. The aim this study was investigate physiological behavioral changes regulatory mechanisms trehalose metabolism M. under conditions high-humidity stress. Additionally, we sought explore survival strategies water regulation employed insect, with goal identifying new biological targets its management. findings indicated that, despite humidity, there no difference rate crassicauda. However, reduction developmental duration reproductive capacity observed. Increased correlated elevated levels decreased glycogen content. Notably, although relative expression trehalase (TRE) Trehalose-6-phosphate synthase (TPS) were downregulated, phosphatase (TPP) upregulated. These results suggest that environments significantly influence growth, development, It appears adaptations are facilitated modulations types distribution sugars within their bodies, achieved through alterations genes associated metabolism. summary, indicate affects development sugar may represent one potential underlying adaptation migration. This insight provides valuable assistance predicting occurrence migration pest

Язык: Английский