Potential Chondroprotective Effect of Artemisia annua L. Water Extract on SW1353 Cell DOI Open Access
Jong Min Kim, Ye Yang,

Ji Woong Heo

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 1901 - 1901

Опубликована: Фев. 22, 2025

Inflammation plays a critical role in the pathogenesis of osteoarthritis (OA). The objective this study was to investigate anti-inflammatory and chondroprotective properties Artemisia annua L. water extract (AWE) following induction inflammation cartilage cells (SW1353 cell) through administration interleukin-1 beta (IL-1β). We demonstrated significant antioxidant activity, as evidenced by elevated total phenolic flavonoid content, addition robust free radical scavenging capacity, assessed DPPH (2,2-diphenyl-1-picrylhydrazyl) ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays. Its cytotoxic effects were at concentration 200 μg/mL, where no signs observed SW1353 treated with IL-1β; levels reactive oxygen species (ROS) notably reduced dose-dependent manner. principal inflammatory markers, cyclooxygenase-2 (COX-2) inducible nitric oxide synthase (iNOS), significantly diminished AWE treatment. led reduction expression key proteins involved mitogen-activated protein kinase (MAPK) nuclear factor kappa-light-chain-enhancer activated B cell (NF-κB) signaling pathways, ultimately resulting decrease release matrix metalloproteinases (MMPs), specifically MMP-1 MMP-13, which are known contribute degradation. Additionally, degraded collagen type II restored. These findings suggest that reducing oxidative stress inflammation, along inhibiting MAPK NF-κB may ameliorate progression IL-1β-induced OA. Furthermore, molecular docking analysis revealed strong binding affinity mediator Six compounds identified AWE, corroborating its potential effects. Therefore, serve potentially useful therapeutic agent against OA modulating inflammation-related mechanisms.

Язык: Английский

Potential Chondroprotective Effect of Artemisia annua L. Water Extract on SW1353 Cell DOI Open Access
Jong Min Kim, Ye Yang,

Ji Woong Heo

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 1901 - 1901

Опубликована: Фев. 22, 2025

Inflammation plays a critical role in the pathogenesis of osteoarthritis (OA). The objective this study was to investigate anti-inflammatory and chondroprotective properties Artemisia annua L. water extract (AWE) following induction inflammation cartilage cells (SW1353 cell) through administration interleukin-1 beta (IL-1β). We demonstrated significant antioxidant activity, as evidenced by elevated total phenolic flavonoid content, addition robust free radical scavenging capacity, assessed DPPH (2,2-diphenyl-1-picrylhydrazyl) ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays. Its cytotoxic effects were at concentration 200 μg/mL, where no signs observed SW1353 treated with IL-1β; levels reactive oxygen species (ROS) notably reduced dose-dependent manner. principal inflammatory markers, cyclooxygenase-2 (COX-2) inducible nitric oxide synthase (iNOS), significantly diminished AWE treatment. led reduction expression key proteins involved mitogen-activated protein kinase (MAPK) nuclear factor kappa-light-chain-enhancer activated B cell (NF-κB) signaling pathways, ultimately resulting decrease release matrix metalloproteinases (MMPs), specifically MMP-1 MMP-13, which are known contribute degradation. Additionally, degraded collagen type II restored. These findings suggest that reducing oxidative stress inflammation, along inhibiting MAPK NF-κB may ameliorate progression IL-1β-induced OA. Furthermore, molecular docking analysis revealed strong binding affinity mediator Six compounds identified AWE, corroborating its potential effects. Therefore, serve potentially useful therapeutic agent against OA modulating inflammation-related mechanisms.

Язык: Английский

Процитировано

0