Exosomes: A Promising Drug Delivery Tool in Hepatic Drug Delivery

Journal of Drug Delivery Science and Technology, Год журнала: 2025, Номер unknown, С. 106761 - 106761

Опубликована: Март 1, 2025

Язык: Английский

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Cost-effective isolation of Viburnum opulus-derived nanovesicles and evaluation of their cytotoxic, anticancer, and antioxidant properties on human glioblastoma cell line U87MG

Medical Oncology, Год журнала: 2025, Номер 42(4)

Опубликована: Март 17, 2025

Abstract Glioblastoma is the most common and highly invasive glial tumor, significantly reducing patient survival. Current therapeutic approaches have limited success rates. Plant-derived nanovesicles are a rapidly developing area, recognized for their exceptional biofunctional properties, emerging as promising approach in cancer treatment. The present study focuses on isolation of from Viburnum opulus fruits using cost-effective method that includes polymer-based exosome precipitation buffer size exclusion chromatography, followed by characterization. Morphological analysis via Field Emission Scanning Electron Microscopy Transmission revealed ranging oval to elliptical shapes, with average diameters 54.23 nm 41.21 nm, respectively. Dynamic light scattering determined 45.36 indicating presence nanovesicles, zeta potential was − 2.87 mV. Biochemical characterization showed total protein phenolic concentrations 1534 ± 97.78 µg/ml 4.270 0.66 mg gallic acid equivalents/L, respectively, antioxidant status values 3.83 0.37 mmol Trolox equivalents/L. Based IC50 values, these were 7.5 times more toxic U87MG human glioblastoma cells compared healthy dermal fibroblasts. Analyses including clonogenic cell survival, wound healing, apoptosis, status, oxidant continued only cells, fibroblasts low response nanovesicle Qualitative quantitative assessments demonstrated -derived effectively inhibited proliferation migration. Due non-toxic, anticancer, hold significant management. Graphical abstract

Язык: Английский

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Regulation of Intestinal Barrier Function and Gut Microbiota by Hot Melt Extrusion-Drug Delivery System-Prepared Mulberry Anthocyanin in an Inflammatory Bowel Disease Model

Pharmaceuticals, Год журнала: 2025, Номер 18(4), С. 475 - 475

Опубликована: Март 27, 2025

Background/Objectives: Anthocyanins (ACNs) derived from mulberry (Morus alba L.) exhibit potent antioxidant and anti-inflammatory activities. However, their low stability bioavailability in physiological environments limit therapeutic potential. This study aimed to enhance the controlled release ACNs using a hot-melt extrusion drug delivery system (HME-DDS) formulation, HME-MUL-F2, evaluate its effects on gut barrier function microbiota composition DSS-induced colitis model. Methods: The anthocyanin content of HME-MUL-F2 was quantified compared with that raw extract. formulation’s protective were assessed Caco-2 RAW 264.7 cells, confirming biocompatibility properties. efficacy further evaluated dextran sulfate sodium (DSS)-induced inflammatory bowel disease (IBD) model, focusing integrity, cytokine modulation, composition. Results: significantly improved by upregulating tight junction proteins reducing levels Moreover, formulation modulated composition, promoting beneficial bacteria while suppressing pathogenic strains. administration led significant increase Bacteroidetes-to-Firmicutes ratio, which is associated health. These results indicate enhances bioavailability, leading health potential applications for conditions. Conclusions: highlights HME technology improving stability, anthocyanins. sustained-release modulates intestinal microbial balance findings strongly suggest observed are primarily due enhanced targeted colon, although clinical studies will provide more definitive confirmation.

Язык: Английский

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Highland barley ELNs and physiological responses to different concentrations of Cr (VI) stress

Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 288, С. 117379 - 117379

Опубликована: Ноя. 24, 2024

Язык: Английский

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