Umckalin Promotes Melanogenesis in B16F10 Cells Through the Activation of Wnt/β-Catenin and MAPK Signaling Pathways DOI Creative Commons

So-Yeon Oh,

Chang‐Gu Hyun

Applied Biosciences, Год журнала: 2025, Номер 4(2), С. 20 - 20

Опубликована: Апрель 2, 2025

Melanogenesis is regulated by melanogenic enzymes such as tyrosinase (TYR), TRP-1, and TRP-2, whose expression controlled the microphthalmia-associated transcription factor (MITF). Various signaling pathways, including cAMP/PKA, MAPK/ERK, Wnt/β-catenin, PI3K/Akt, are involved in this process have been a focal point of research for treating pigmentation disorders. However, developing effective therapies conditions like vitiligo remains significant challenge. In study, effects umckalin on melanogenesis its molecular mechanisms were investigated using B16F10 cells, mouse melanoma cell line widely used model melanin production studies. cells produce via melanosomes express key TYR, making them reliable system. Our findings demonstrate that promotes concentration-dependent manner upregulating TRP-1 activating MITF pathway. Additionally, modulated GSK3β/β-catenin MAPK, to enhance melanogenesis. conclusion, enhances enzyme activity critical thereby promoting synthesis. These suggest could be promising candidate therapeutic agents disorders vitiligo. Further studies required explore clinical applications greater detail.

Язык: Английский

Drug Repurposing of Voglibose, a Diabetes Medication for Skin Health DOI Creative Commons

Hyeonmi Kim,

Chang‐Gu Hyun

Pharmaceuticals, Год журнала: 2025, Номер 18(2), С. 224 - 224

Опубликована: Фев. 7, 2025

Background/Objectives: Voglibose, an α-glucosidase inhibitor commonly prescribed to manage postprandial hyperglycemia in diabetes mellitus, demonstrates potential for repurposing as anti-melanogenic agent. This study aims explore the inhibitory effects of voglibose on melanogenesis and elucidate its molecular mechanisms, highlighting possible applications treating hyperpigmentation disorders. Methods: The were investigated using B16F10 melanoma cells. Cell viability, melanin content, tyrosinase activity assessed following treatment. Western blot analysis was performed examine changes melanogenic proteins transcription factors. role signaling pathways, including PKA/CREB, MAPK, PI3K/AKT, GSK3β/β-Catenin, analyzed. Primary human skin irritation tests conducted evaluate topical safety voglibose. Results: Voglibose significantly reduced synthesis cells a dose-dependent manner. revealed decreased expression MITF, TRP-1, TRP-2, indicating inhibition melanogenesis. modulated key suppression AKT activation, while restoring GSK3β inhibit β-catenin stabilization. Human confirmed voglibose’s application, showing no adverse reactions at 50 100 μM concentrations. Conclusions: properties through modulation multiple pathways biosynthesis. Its profile efficacy suggest repurposed drug managing advancing cosmeceutical applications.

Язык: Английский

Процитировано

1
Rifampicin Repurposing Reveals Anti-Melanogenic Activity in B16F10 Melanoma Cells DOI Creative Commons

Y. Terry Lee,

Chang‐Gu Hyun

Molecules, Год журнала: 2025, Номер 30(4), С. 900 - 900

Опубликована: Фев. 15, 2025

Drug repurposing is a cost-effective and innovative strategy for identifying new therapeutic applications existing drugs, thereby shortening development timelines accelerating the availability of treatments. Applying this approach to cosmeceutical ingredients enables creation functional compounds with proven safety efficacy, adding significant value cosmetic industry. This study evaluated potential rifampicin, drug widely used treatment tuberculosis leprosy, as agent. The anti-melanogenic effects rifampicin were assessed in B16F10 melanoma cells, showing no cytotoxicity at concentrations up 40 µM reduction intracellular tyrosinase activity melanin content. Mechanistically, reduced expression melanogenic enzymes, including tyrosinase, tyrosinase-related protein (TRP)-1, TRP-2, via kinase A (PKA)-dependent pathway, leading suppression microphthalmia-associated transcription factor (MITF), which key regulator melanogenesis. Additionally, inhibited p38 signaling pathway but was independent PI3K/protein B (Akt) pathway. Furthermore, it decreased Ser9 phosphorylation, enhancing glycogen synthase kinase-3β (GSK-3β) activity, promoted β-catenin facilitated degradation, collectively contributing inhibition synthesis. To evaluate topical applicability primary human skin irritation tests conducted, adverse observed 20 µM. These findings demonstrate that inhibits melanogenesis through multiple pathways, PKA, MAPKs, GSK-3β/β-catenin. highlights be repurposed agent managing hyperpigmentation disorders, offering valuable insights into novel strategies pigmentation-related conditions.

Язык: Английский

Процитировано

0
Umckalin Promotes Melanogenesis in B16F10 Cells Through the Activation of Wnt/β-Catenin and MAPK Signaling Pathways DOI Creative Commons

So-Yeon Oh,

Chang‐Gu Hyun

Applied Biosciences, Год журнала: 2025, Номер 4(2), С. 20 - 20

Опубликована: Апрель 2, 2025

Melanogenesis is regulated by melanogenic enzymes such as tyrosinase (TYR), TRP-1, and TRP-2, whose expression controlled the microphthalmia-associated transcription factor (MITF). Various signaling pathways, including cAMP/PKA, MAPK/ERK, Wnt/β-catenin, PI3K/Akt, are involved in this process have been a focal point of research for treating pigmentation disorders. However, developing effective therapies conditions like vitiligo remains significant challenge. In study, effects umckalin on melanogenesis its molecular mechanisms were investigated using B16F10 cells, mouse melanoma cell line widely used model melanin production studies. cells produce via melanosomes express key TYR, making them reliable system. Our findings demonstrate that promotes concentration-dependent manner upregulating TRP-1 activating MITF pathway. Additionally, modulated GSK3β/β-catenin MAPK, to enhance melanogenesis. conclusion, enhances enzyme activity critical thereby promoting synthesis. These suggest could be promising candidate therapeutic agents disorders vitiligo. Further studies required explore clinical applications greater detail.

Язык: Английский

Процитировано

0