Parabacteroides johnsonii Inhibits the Onset and Progression of Colorectal Cancer by Modulating the Gut Microbiota DOI
Jing Liu, Yong Zhang, Li‐Yan Xu

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Апрель 7, 2025

Abstract Background Colorectal cancer (CRC) is the third most prevalent malignant tumor and second leading cause of cancer-related deaths globally. The genus Parabacteroides an important component gut microbiota. P. distasonis goldsteinii are reported probiotics, their roles in CRC have been investigated related studies. However, association between johnsonii remains unknown. Methods (10–42) Lactococcus formosensis (22–2) were isolated from healthy human feces. 25 mice that demonstrated normal feeding activity randomly assigned to four groups : control (NC group), model (IC (PJ L. (LO group). Colonic tissues IC, PJ, LO colon NC group then collected for HE staining immunohistochemical staining. Fecal samples during hyperproliferative adenoma phases Metagenomic sequencing metabolite analysis. Results intervention reduced number slowed growth colonic tumors, improved histological scores, decreased microenvironmental inflammation levels. composition intestinal flora with cancer, increased microbial species diversity, maintained microbiota stability. Furthermore, abundance Bifidobacterium pseudolongum Lactobacillus, which play a role ameliorating AOM/DSS-induced dysbiosis. affected metabolic pathways, including amino sugar degradation galactose metabolism, sphingolipid synthesis, acid polyphenol synthesis tryptophan metabolism pathway as primary being affected. Conclusion Our study profiled administration reduces tumors lower staging by modulating its metabolites at early stages.

Язык: Английский

Oxidative and Excitatory Neurotoxic Stresses in CRISPR/Cas9-Induced Kynurenine Aminotransferase Knockout Mice: A Novel Model for Despair-Based Depression and Post-Traumatic Stress Disorder DOI Creative Commons
Ágnes Szabó, Zsolt Galla, Eleonóra Spekker

и другие.

Frontiers in Bioscience-Landmark, Год журнала: 2025, Номер 30(1)

Опубликована: Янв. 20, 2025

Backgrounds: Memory and emotion are especially vulnerable to psychiatric disorders such as post-traumatic stress disorder (PTSD), which is linked disruptions in serotonin (5-HT) metabolism. Over 90% of the 5-HT precursor tryptophan (Trp) metabolized via Trp-kynurenine (KYN) metabolic pathway, generates a variety bioactive molecules. Dysregulation KYN metabolism, particularly low levels kynurenic acid (KYNA), appears be neuropsychiatric disorders. The majority KYNA produced by aadat (kat2) gene-encoded mitochondrial kynurenine aminotransferase (KAT) isotype 2. Little known about consequences deleting enzyme gene. Methods: In CRISPR/Cas9-induced knockout (kat2-/-) mice, we examined effects on emotion, memory, motor function, Trp its metabolite levels, activities plasma urine 8-week-old males compared wild-type mice. Results: Transgenic mice showed more depressive-like behaviors forced swim test, but not tail suspension, anxiety, or memory tests. They also had fewer center field corner entries, shorter walking distances, jumping counts open test. Plasma generally consistent with those urine: antioxidant KYNs, 5-hydroxyindoleacetic acid, indole-3-acetic were lower; KATs, kynureninase, monoamine oxidase/aldehyde dehydrogenase lower, 3-monooxygenase was higher; oxidative excitotoxicity indices higher. displayed depression-like behavior learned helplessness model, emotional indifference, deficits, coupled decrease KYNA, shift metabolism toward KYN-3-hydroxykynurenine partial gut microbial Trp-indole pathway metabolite. Conclusions: This first evidence that gene induces uniquely experiences despair, appear associated excitatory neurotoxic stresses. may lead development double-hit preclinical model despair-based depression, better understanding these complex conditions, effective therapeutic strategies elucidating relationship between PTSD pathogenesis.

Язык: Английский

Процитировано

1
Exercise-induced adaptations in the kynurenine pathway: implications for health and disease management DOI Creative Commons
Marcus Vinícius dos Santos Rangel, Karynne Grutter Lopes, Xuebin Qin

и другие.

Frontiers in Sports and Active Living, Год журнала: 2025, Номер 7

Опубликована: Март 6, 2025

Background Tryptophan (TRP) metabolism through the kynurenine (KYN) pathway is influenced by inflammatory mediators, generating metabolites that regulate immune and responses. Exercise has been proposed as a modulator of this pathway, but its role in health benefits chronic disease management remains unclear. Objective This systematic review examines exercise-induced adaptations KYN their potential implications for management. Additionally, we identify key methodological considerations future research. Methods A structured search PubMed/Medline, Web Science, Scopus was conducted up to October 2024 clinical trials investigating effects exercise training on pathway. Results Of 2,795 articles initially found, 13 involving 592 participants met inclusion criteria. Most studies reported particularly cancer survivors. These appeared be intensity duration. However, several limitations were noted, no included patients with metabolic or cardiovascular diseases. Conclusions Here, show modulates both healthy diseased populations, highlighting prevention further randomized-controlled are needed clarify mechanisms applications, Systematic Review Registration https://www.crd.york.ac.uk/PROSPERO/view/CRD42022351481 , PROSPERO (CRD42022351481).

Язык: Английский

Процитировано

0
Shenqi granules enhance recovery from cerebral ischemia-reperfusion injury by modulating tryptophan and tyrosine metabolism and activating NFE2L2/NRF2 DOI

Hai‐Xin Liu,

Mihi Yang, Yuchang Li

и другие.

Phytomedicine, Год журнала: 2025, Номер 140, С. 156623 - 156623

Опубликована: Март 10, 2025

Язык: Английский

Процитировано

0
Parabacteroides johnsonii Inhibits the Onset and Progression of Colorectal Cancer by Modulating the Gut Microbiota DOI
Jing Liu, Yong Zhang, Li‐Yan Xu

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Апрель 7, 2025

Abstract Background Colorectal cancer (CRC) is the third most prevalent malignant tumor and second leading cause of cancer-related deaths globally. The genus Parabacteroides an important component gut microbiota. P. distasonis goldsteinii are reported probiotics, their roles in CRC have been investigated related studies. However, association between johnsonii remains unknown. Methods (10–42) Lactococcus formosensis (22–2) were isolated from healthy human feces. 25 mice that demonstrated normal feeding activity randomly assigned to four groups : control (NC group), model (IC (PJ L. (LO group). Colonic tissues IC, PJ, LO colon NC group then collected for HE staining immunohistochemical staining. Fecal samples during hyperproliferative adenoma phases Metagenomic sequencing metabolite analysis. Results intervention reduced number slowed growth colonic tumors, improved histological scores, decreased microenvironmental inflammation levels. composition intestinal flora with cancer, increased microbial species diversity, maintained microbiota stability. Furthermore, abundance Bifidobacterium pseudolongum Lactobacillus, which play a role ameliorating AOM/DSS-induced dysbiosis. affected metabolic pathways, including amino sugar degradation galactose metabolism, sphingolipid synthesis, acid polyphenol synthesis tryptophan metabolism pathway as primary being affected. Conclusion Our study profiled administration reduces tumors lower staging by modulating its metabolites at early stages.

Язык: Английский

Процитировано

0