Transcriptome Profiling Reveals the Effects of Rootstocks on Scion Architecture in Malus domestica Borkh Var. ‘Harlikar’ DOI Creative Commons

Bin Xie,

Junhao Li, Jiangtao Zhou

и другие.

Plants, Год журнала: 2025, Номер 14(5), С. 696 - 696

Опубликована: Фев. 24, 2025

Rootstocks largely determine the tree architecture of grafted scions, significantly affects yield, suitability for mechanical harvesting, and planting pattern apple orchards. It is thus important to reveal mechanisms behind rootstocks influence on scions in trees. This study analyzed grafting survival rate, physiological parameters including plant growth, photosynthesis nutrient accumulation variety ‘Harlikar’ with eight rootstocks. We also explored mechanism scion formation using transcriptomics based different scion/rootstock combinations. The results indicated that had lowest rate rootstock ‘M26’, less callus formed at graft interface, foliage etiolation, weak photosynthetic capacity. While better affinities ‘M9-T337’, ‘M9-Nic29’, ‘M9-Pajam2’, ‘B9’, ‘71-3-150’, ‘Qingzhen 2’, ‘Malus baccata’. Among these, highest height number lateral branches were observed they 1.12-times 2.0-times higher than vigorous ‘M. baccata’, respectively. accumulations total nitrogen, phosphorus, potassium ‘Harlikar’/‘Qingzhen 2.22-times, 2.10-times, 11.80-times ‘Harlikar’/‘M. was ‘Harlikar’/‘71-3-150’, only 50.47% 2’ 56.51% internode length ‘Harlikar’/‘M9-Nic29’, 60.76% 79.11% transcriptome, weighted gene co-expression network KEGG enrichment analyses revealed that, compared most differentially expressed genes screened from ‘Harlikar’/‘M9-Nic29’ enriched hormone signal transduction pathways. Specifically, auxin-repressed protein ARP, cytokinin synthesis related CKXs CYP92A6, brassinosteroid CYP87A3 involved dwarfing ‘Harlikar’/‘71-3-150’ ‘Harlikar’/‘M9-Nic29’. Cytokinin ARR-A abscisic acid-responsive element binding factor ABF key increased branching 2’. In addition, acid phosphatase ACPs, serine/threonine-protein kinase PBLs vegetative growth by affecting absorption utilization nutrients. These provide theoretical guidance cultivating high-quality trees elucidate molecular regulating branch apple.

Язык: Английский

Transcriptome Profiling Reveals the Effects of Rootstocks on Scion Architecture in Malus domestica Borkh Var. ‘Harlikar’ DOI Creative Commons

Bin Xie,

Junhao Li, Jiangtao Zhou

и другие.

Plants, Год журнала: 2025, Номер 14(5), С. 696 - 696

Опубликована: Фев. 24, 2025

Rootstocks largely determine the tree architecture of grafted scions, significantly affects yield, suitability for mechanical harvesting, and planting pattern apple orchards. It is thus important to reveal mechanisms behind rootstocks influence on scions in trees. This study analyzed grafting survival rate, physiological parameters including plant growth, photosynthesis nutrient accumulation variety ‘Harlikar’ with eight rootstocks. We also explored mechanism scion formation using transcriptomics based different scion/rootstock combinations. The results indicated that had lowest rate rootstock ‘M26’, less callus formed at graft interface, foliage etiolation, weak photosynthetic capacity. While better affinities ‘M9-T337’, ‘M9-Nic29’, ‘M9-Pajam2’, ‘B9’, ‘71-3-150’, ‘Qingzhen 2’, ‘Malus baccata’. Among these, highest height number lateral branches were observed they 1.12-times 2.0-times higher than vigorous ‘M. baccata’, respectively. accumulations total nitrogen, phosphorus, potassium ‘Harlikar’/‘Qingzhen 2.22-times, 2.10-times, 11.80-times ‘Harlikar’/‘M. was ‘Harlikar’/‘71-3-150’, only 50.47% 2’ 56.51% internode length ‘Harlikar’/‘M9-Nic29’, 60.76% 79.11% transcriptome, weighted gene co-expression network KEGG enrichment analyses revealed that, compared most differentially expressed genes screened from ‘Harlikar’/‘M9-Nic29’ enriched hormone signal transduction pathways. Specifically, auxin-repressed protein ARP, cytokinin synthesis related CKXs CYP92A6, brassinosteroid CYP87A3 involved dwarfing ‘Harlikar’/‘71-3-150’ ‘Harlikar’/‘M9-Nic29’. Cytokinin ARR-A abscisic acid-responsive element binding factor ABF key increased branching 2’. In addition, acid phosphatase ACPs, serine/threonine-protein kinase PBLs vegetative growth by affecting absorption utilization nutrients. These provide theoretical guidance cultivating high-quality trees elucidate molecular regulating branch apple.

Язык: Английский

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