Lipidomic profile of meningiomas harboring different NF2 mutation status DOI
Joanna Bogusiewicz, I. Stanimirova, Magdalena Gaca-Tabaszewska

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Апрель 29, 2025

Abstract Introduction: Meningiomas are mainly benign brain tumors, but they can evolve to higher grades. The phenomena of these changes not well-known. Therefore, more basic research is needed. This study attempted assess the lipidome profile in meningiomas harboring different NF2 mutation statuses (wildtype and mutated). Solid-phase microextraction (SPME) probes were used sample extract metabolites reduce invasiveness lipidomic analysis. Objectives: aimed select set lipids distinguishing with genotypes using two chromatography methods (hydrophilic interaction (HILIC) reversed-phase (RPLC) ionization modes. Methods: Brain tumors obtained during neurosurgical procedures. Then, sampling SPME fibers was performed directly after lesion excision. After collecting whole batch samples, desorption an isopropanol-methanol solution performed. Subsequently, instrumental analysis carried out liquid coupled high-resolution mass spectrometry. remaining part stored as paraffin tissue blocks, then genetic testing determine presence mutations gene. Results: Genetic profiling revealed that most lesions had a A wide range analytes extracted from studied probes, it possible 26 crucial tumor differentiation. It also observed combination detected than one mode increased differentiation mutant wildtype which presented by high sensitivity specificity prepared models. Conclusions: spectrometry, be successfully applied screening NF statuses.

Язык: Английский

Lipidomic profile of meningiomas harboring different NF2 mutation status DOI
Joanna Bogusiewicz, I. Stanimirova, Magdalena Gaca-Tabaszewska

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Апрель 29, 2025

Abstract Introduction: Meningiomas are mainly benign brain tumors, but they can evolve to higher grades. The phenomena of these changes not well-known. Therefore, more basic research is needed. This study attempted assess the lipidome profile in meningiomas harboring different NF2 mutation statuses (wildtype and mutated). Solid-phase microextraction (SPME) probes were used sample extract metabolites reduce invasiveness lipidomic analysis. Objectives: aimed select set lipids distinguishing with genotypes using two chromatography methods (hydrophilic interaction (HILIC) reversed-phase (RPLC) ionization modes. Methods: Brain tumors obtained during neurosurgical procedures. Then, sampling SPME fibers was performed directly after lesion excision. After collecting whole batch samples, desorption an isopropanol-methanol solution performed. Subsequently, instrumental analysis carried out liquid coupled high-resolution mass spectrometry. remaining part stored as paraffin tissue blocks, then genetic testing determine presence mutations gene. Results: Genetic profiling revealed that most lesions had a A wide range analytes extracted from studied probes, it possible 26 crucial tumor differentiation. It also observed combination detected than one mode increased differentiation mutant wildtype which presented by high sensitivity specificity prepared models. Conclusions: spectrometry, be successfully applied screening NF statuses.

Язык: Английский

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