Exploring the Toxicity and Therapeutic Potential of A. dahurica and A. pubescens in Zebrafish Larvae: Insights into Anxiety Treatment Mechanisms

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 2884 - 2884

Опубликована: Март 22, 2025

This study assessed the toxicity and therapeutic potential of Angelica dahurica pubescens using Danio rerio (zebrafish) larvae. Toxicity was evaluated through mortality, malformations, gene expression changes related to stress HPA axis. A. demonstrated low (LD50 (50% lethal dose) >200 µg/mL), with no significant malformations at 15-30 µg/mL, although higher doses caused edemas heart defects. exhibited toxicity, 100% mortality 200 µg/mL severe malformations. Both species showed cardiotoxicity, slowing rates after prolonged exposure. Gene studies suggested had stress-protective effects, increasing nr3c1 expression, while dose-dependent lower concentrations having anxiolytic properties stress. Interestingly, diazepam unexpected changes, highlighting influence environmental dosage factors. In conclusion, both show for anxiety, showing promising effects concentrations. However, requires careful management due its risks. Further are needed optimize applications fully understand mechanisms action.

Язык: Английский

Epigenetic Echoes: Bridging Nature, Nurture, and Healing Across Generations

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3075 - 3075

Опубликована: Март 27, 2025

Trauma can impact individuals within a generation (intragenerational) and future generations (transgenerational) through complex interplay of biological environmental factors. This review explores the epigenetic mechanisms that have been correlated with effects trauma across generations, including DNA methylation, histone modifications, non-coding RNAs. These regulate expression stress-related genes (such as glucocorticoid receptor (NR3C1) FK506 binding protein 5 (FKBP5) gene), linking to pathways may affect long-term stress regulation health outcomes. Although research using model organisms has elucidated potential underlying intergenerational trauma, applying these findings human populations remains challenging due confounding variables, methodological limitations, ethical considerations. complexity is compounded by difficulties in establishing causality disentangling influences from shared Emerging therapies, such psychedelic-assisted treatments mind-body interventions, offer promising avenues address both psychological aspects trauma. However, translating into effective interventions will require interdisciplinary methods culturally sensitive approaches. Enriched environments, cultural reconnection, psychosocial shown mitigate trauma's impacts generations. By integrating biological, social, perspectives, this highlights critical importance frameworks breaking cycles fostering resilience, advancing comprehensive healing

Язык: Английский