Validation and Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis in Mythimna loreyi (Lepidoptera: Noctuidae)
Insects,
Год журнала:
2024,
Номер
15(3), С. 185 - 185
Опубликована: Март 10, 2024
Quantitative
real-time
PCR
(qRT-PCR)
is
a
widely
applied
technique
for
accurately
assessing
the
expression
of
target
genes.
In
practice,
evaluation
gene
requires
appropriate
reference
To
screen
reliable
genes
evaluating
via
qRT-PCR
in
Mythimna
loreyi,
notorious
migratory
pest
across
Asia,
Africa,
Europe,
and
Australia,
we
assessed
stability
13
candidate
M.
loreyi
using
ΔCt
method,
BestKeeper,
Normfinder,
GeNorm,
web-based
comprehensive
platform
RefFinder.
These
include
RPL10,
RPL27,
RPL32,
RPS3,
TATA−box,
GAPDH,
AK,
Actin,
EF,
α−tubulin,
SOD,
18S
rRNA,
FTZ−F1,
which
frequently
employed
Lepidoptera
insects.
Our
findings
revealed
that
performance
depended
on
experimental
conditions.
Specifically,
RPL27
RPL10
were
most
suitable
changes
developmental
stages,
tissues,
adult
ages.
The
optimal
recommended
specific
experiment
conditions,
instance,
EF
RPS3
mating
status,
AK
temperature
treatments,
FTZ−F1
larva
diet,
diet
treatments.
Additionally,
profiles
pheromone-binding
protein
2
(MlorPBP2)
glutathione
S-transferase
(MlorGST1)
used
to
validate
This
study
provides
accurate
normalization
data,
laying
groundwork
studying
loreyi.
Язык: Английский
Characterization of the ligand-binding properties of odorant-binding protein 38 from Riptortus pedestris when interacting with soybean volatiles
Frontiers in Physiology,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 6, 2025
Riptortus
pedestris
(Fabricius)
(Hemiptera:
Alydidae)
is
a
major
soybean
pest
throughout
East
Asia
that
relies
on
its
advanced
olfactory
system
for
the
perception
of
plant-derived
volatile
compounds
and
aggregation
pheromones
conspecific
host
plant
localization.
Odorant
binding
proteins
(OBPs)
facilitate
transport
odorant
across
sensillum
lymph
within
insect
system,
enabling
their
interaction
with
receptors
(ORs).
Real-time
quantitative
PCR
(qRT-PCR)
analyses,
fluorescence-based
competitive
assays,
molecular
docking
analyses
were
applied
to
assess
expression
ligand-binding
properties
OBP38
from
R.
peddestris.
The
qRT-PCR
revealed
high
levels
RpedOBP38
in
antennae
without
any
apparent
sex
bias,
it
was
also
highly
expressed
adult
stage.
Recombinant
prepared
by
expressing
E.
coli
BL21
(DE3)
followed
purification
Ni-chelating
affinity
column.
found
bind
most
strongly
trans-2-decenal
(Ki
=
7.440)
trans-2-nonenal
10.973),
β-pinene,
(+)
-4-terpineol,
carvacrol,
methyl
salicylate,
(-)-carvone.
3D
structure
contains
six
α-helices
three
interlocked
disulfide
bridges
comprising
stable
hydrophobic
pocket.
In
final
series
several
polar
(e.g.,
His
94,
Glu97)
nonpolar
Leu29,
Ile59)
residues
be
involved
RpedOBP38-ligand
binding.
These
data
support
role
volatiles
derived
plants,
providing
important
insight
into
mechanisms
govern
recognition
pedestris,
thereby
informing
development
ecologically
friendly
approaches
managing
infestations.
Язык: Английский
Evaluation and Validation of Reference Genes for Gene Expression Analysis Using qRT-PCR in the Sugarcane Stem Borer Chilo sacchariphagus (Lepidoptera: Pyralidae)
Insects,
Год журнала:
2024,
Номер
15(8), С. 594 - 594
Опубликована: Авг. 4, 2024
(Lepidoptera:
Pyralidae)
is
an
economically
important
sugarcane
pest.
Although
numerous
studies
were
conducted
on
the
physiological
responses
in
Язык: Английский
Comprehensive Screening and Validation of Stable Internal Reference Genes for Accurate qRT-PCR Analysis in Holotrichia parallela under Diverse Biological Conditions and Environmental Stresses
Insects,
Год журнала:
2024,
Номер
15(9), С. 661 - 661
Опубликована: Авг. 30, 2024
is
among
the
world's
most
destructive
pests.
For
accurate
qPCR
and
gene
expression
studies,
selection
of
stable
appropriate
reference
genes
crucial.
However,
a
thorough
evaluation
potential
for
use
in
Язык: Английский
Identification of a Soybean Volatile Attractive for Riptortus pedestris Using Reverse Chemical Ecology Approach
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 27, 2024
The
bean
bug
Riptortus
pedestris
is
a
major
soybean
pest
and
cause
of
the
stay-green
symptoms.
However,
molecular
mechanisms
underlying
its
olfaction-mediated
host-seeking
behavior
remain
unclear.
In
this
study,
we
compared
antennae
transcriptomes
starved
nonstarved
adult
R.
pedestris,
identifying
four
differentially
expressed
odorant
receptor
(OR)
genes.
Among
these,
RpedOR13
showed
strong
response
to
host
volatile
2-phenylethanol
(2-PE)
in
Xenopus
oocyte
assays,
while
electroantennography
behavioral
tests
confirmed
2-PE
as
an
effective
attractant.
Next,
phylogenetic
analysis
identified
RpedOR72b
paralog
RpedOR13,
with
subsequent
assays
confirming
specific
2-PE.
Additionally,
RNA
interference
experiments
highlighted
crucial
role
detecting
Taken
together,
these
findings
provide
new
insights
into
highlight
successful
application
reverse
chemical
ecology
OR-based
screening
bioactive
compounds.
Язык: Английский
Screening and Validation of Stable Reference Genes for qRT-PCR Analysis in Epicauta gorhami (Coleoptera: Meloidae)
Insects,
Год журнала:
2024,
Номер
15(12), С. 942 - 942
Опубликована: Ноя. 29, 2024
Epicauta
gorhami
is
a
hypermetamorphic
insect
that
mainly
forage
soybeans
during
the
adult
stage.
However,
lack
of
appropriate
references
hinders
our
studying
gene
function
in
E.
gorhami.
In
this
study,
referring
to
five
computational
tools
(Ct
value,
geNorm,
NormFinder,
BestKeeper
and
RefFinder),
stability
10
housekeeping
genes
(GAPDH,
ACT,
RPL4,
RPL27,
α-TUB,
RPS18,
EF1α,
RPS28,
RPL13
SOD)
was
assessed
by
qRT-PCR
under
three
different
conditions
(adult
ages,
tissues/organs
temperatures).
The
findings
suggested
SOD
RPS18
were
most
ideal
for
examine
transcripts
among
diverse
ages
at
various
temperatures;
pair
RPS28
reliable
assess
expressions
tissues.
Finally,
relative
expression
levels
EgUAP
computed
after
normalization
with
across
As
expected,
abundant
foregut,
trachea
antenna
scarce
midgut,
hindgut
epidermis.
These
will
lay
solid
foundation
analyzing
Язык: Английский