The Changes in the Antibiotic Resistance of Staphylococcus aureus, Streptococcus pneumoniae, Enterococcus faecalis and Enterococcus faecium in the Clinical Isolates of a Multiprofile Hospital over 6 Years (2017–2022)

Journal of Clinical Medicine, Год журнала: 2025, Номер 14(2), С. 332 - 332

Опубликована: Янв. 8, 2025

Background/Objectives: The growing resistance of bacteria to antibiotics is a serious problem in health care. present study aims assess the drug Staphylococcus aureus, Enterococcus faecium, faecalis and Streptococcus pneumoniae isolated from infections multispecialty hospital over 6-year period. Methods: Identification antimicrobial susceptibility testing were performed using VITEK®2 automated system (Biomerieux). Results Conclusions: Based on data analyzed hospital, MRSA strains etiological factor 18-28% S. aureus infections. In each year 2017 2022, percentage MSSA steadily exceeded number strains. show significant sensitivity antibiotic groups other than β-lactams, such as aminoglycosides, tetracyclines, cotrimoxazole, linezolid vancomycin. Ciprofloxacin which displays second-highest resistance, after methicillin. case strains, almost 100% lack quinolines was found. An increase caused by genus observed. For E. faecium vancomycin-resistant reached much 41% 2018. Among resistant faecalis, VREs (Vancomycin-Resistant Enterococci) slightly predominate, while GREs (Glycopeptide-Resistant are more prevalent faecium. that insensitive ampicillin ranged 6% 17%. 2017, this 17%, their share decreased 9%. 2021-2022, erythromycin high 33%. This related MLS (macrolides, lincosamides, streptogramines B) mechanism. observed 2019. six-year period, 2020 (beginning pandemic), some antibiotics, consumption DDD/100 person-days recorded. most visible fluoroquinolones. analysis carried out will effectiveness empirical therapy prudent use limit selection multidrug-resistant

Язык: Английский

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Genomic Insights into Colistin and Tigecycline Resistance in ESBL-Producing Escherichia coli and Klebsiella pneumoniae Harboring blaKPC Genes in Ecuador

Antibiotics, Год журнала: 2025, Номер 14(2), С. 206 - 206

Опубликована: Фев. 17, 2025

Introduction:Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) are resistant to third-generation cephalosporins (3GCs), carbapenems, colistin, tigecycline, making them a major public health priority, mainly within the developing world. However, their genomic epidemiology possible determinants of resistance remain be elucidated. Thus, this study aimed perform characterization E. K. pneumoniae, both which last-line antibiotics, isolated from humans, poultry, dairy farm environment Ecuador. Methods: This analyzed nine 3GC-resistant isolates harboring mcr-1 gene (six poultry farms, two human infections, one compost), together with ten colistin- carbapenem-resistant clinical samples. Results: The origin belonged ST609 phylogroup A, while compost phylogroups B1, E, F. Diverse STs included ST13 (five isolates), ST258 (four ST86 (one isolate). Within isolates, blaCTX-M-55, blaCTX-M-65, blaCTX-M-15, blaCTX-M-2 genes were identified. also identified blaCMY-2 blaKPC-3 (the latter in carbapenem-susceptible In coli, plasmid-borne mcr-1.1 was across all an IncI2 plasmid. Tigecycline-reduced susceptibility or related missense amino acid substitutions coded marA acrA genes. pneumoiae, blaCTX-M-15 on hand, blaKPC-2 blaKPC-3, other, associated 3GC carbapenem resistance, respectively. allele ~10 kb Tn4401 transposon (tnpR-tnpA-istA-istB-blaKPC-2-tnpA). K sequence data phenotypic analysis linked nonsense substitution mgrB (K3*) marA, acrA, arnB, eptA, pmrB, pmrJ, phoQ colistin resistance. Meanwhile, tigecycline ramR sequence. Additionally, several integron structures, including Int191 (5'CS-dfrA14-3'CS), most prevalent (Int) among study, followed by Int0 (5'CS-3'CS) Int18 (5'CS-dfrA1-3'CS). Conclusions: These results contribute MDR our setting worldwide One Health approach.

Язык: Английский

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Challenges in the Treatment of Urinary Tract Infections: Antibiotic Resistance Profiles of Escherichia coli Strains Isolated from Young and Elderly Patients in a Southeastern Romanian Hospital

Biomedicines, Год журнала: 2025, Номер 13(5), С. 1066 - 1066

Опубликована: Апрель 28, 2025

Background/Objectives: Urinary tract infections (UTIs) represent a significant public health challenge, with Escherichia coli being the primary causative pathogen. The rise in antimicrobial resistance (AMR), further intensified by shifts antibiotic prescribing practices during and after COVID-19 pandemic, poses substantial difficulties treatment optimization clinical management. Methods: This retrospective study analyzed 644 E. strains from urine samples collected southeastern Romanian hospital two periods: pre-pandemic (2018–2019, N = 361) post-pandemic (2023–2024, 283). Antimicrobial susceptibility was assessed using VITEK automated system for key classes. Results: A increase fluoroquinolone observed, especially ciprofloxacin (p 0.02), alongside rising ceftriaxone 0.004), suggesting spread of ESBL-producing strains. Resistance to trimethoprim/sulfamethoxazole, ampicillin, amoxicillin/clavulanic acid remained high, limiting their empirical use. Carbapenem low > 0.1), while nitrofurantoin fosfomycin retained high efficacy 0.26 p 0.64). Conclusions: period showed concerning fluoroquinolones third-generation cephalosporins, highlighting need stricter stewardship. Carbapenems remain effective severe infections, are reliable first-line options uncomplicated UTIs. Continuous AMR surveillance is essential optimize curb multidrug-resistant

Язык: Английский

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Changes in Antibiotic Resistance of Acinetobacter baumannii and Pseudomonas aeruginosa Clinical Isolates in a Multi-Profile Hospital in Years 2017–2022 in Wroclaw, Poland

Journal of Clinical Medicine, Год журнала: 2023, Номер 12(15), С. 5020 - 5020

Опубликована: Июль 30, 2023

In recent years, we have witnessed increasing drug resistance among bacteria, which is associated with the use and availability of an number broad-spectrum antimicrobials, as well their irrational excessive use. The present study aims to analyze changes in Gram-negative Pseudomonas aeruginosa Acinetobacter baumannii, isolated from infections a multi-profile hospital over five-year period (from 2017 2022). Among practical results evaluation these data will be possibility determine susceptibility antibiotics used hospital. This, turn, help propose new therapeutic options, especially for empirical therapy, essential severe infections. Analysis different antibiotic groups has made it possible identify causes analyzed bacilli. highest was observed between 2020 2022, most probably due COVID-19 pandemic higher patients condition requiring hospitalization. Unfortunately, during analyzed, multi-resistant strains A. baumannii successively increasing; this seems related increased use, period, antibiotics, mainly penicillins inhibitors, third-generation cephalosporins carbapenems.

Язык: Английский

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Comprehensive Approaches to Combatting Acinetobacter baumannii Biofilms: From Biofilm Structure to Phage-Based Therapies

Antibiotics, Год журнала: 2024, Номер 13(11), С. 1064 - 1064

Опубликована: Ноя. 8, 2024

Acinetobacter baumannii—a multidrug-resistant (MDR) pathogen that causes, for example, skin and soft tissue wounds; urinary tract infections; pneumonia; bacteremia; endocarditis, particularly due to its ability form robust biofilms—poses a significant challenge in clinical settings. This structure protects the bacteria from immune responses antibiotic treatments, making infections difficult eradicate. Given rise resistance, alternative therapeutic approaches are urgently needed. Bacteriophage-based strategies have emerged as promising solution combating A. baumannii biofilms. Phages, which viruses specifically infect bacteria, offer targeted effective means of disrupting biofilm lysing bacterial cells. review explores current advancements bacteriophage therapy, focusing on potential treating biofilm-related infections. We described mechanisms by phages interact with biofilms, challenges phage therapy implementation, being developed enhance efficacy (phage cocktails, engineered phages, combination therapies antibiotics). Understanding role bacteriophages both disruption inhibition forming could pave way innovative treatments MDR well prevention their development.

Язык: Английский

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High incidence of fosfomycin-resistant uropathogenic E. coli among children

BMC Infectious Diseases, Год журнала: 2023, Номер 23(1)

Опубликована: Июль 17, 2023

Abstract Background There are few epidemiological or molecular data on Escherichia coli (E. ) strains resistant to fosfomycin. In this study, we described the occurrence and characterization of fosfomycin-resistant uropathogenic E. (UPEC) isolated from children. Materials methods This study was carried out 96 isolates obtained children with urinary tract infections. Two were performed detect fosfomycin resistance: The agar dilution method rapid test. disc diffusion done antimicrobial susceptibility pattern all isolates. phylogenetic grouping according modified Clermont method. Conventional PCR plasmid-mediated genes ( fos genes) bla CTX−M gene. Results Analyses by SPSS software. A high percentage resistance (37/96; 38.5%) reported among UPEC showed a higher rate than fosfomycin-susceptible different antibiotics. E group (62.2%) most predominant isolates, followed Group B2 (21.6%) D (13.5%). detected in 21 A3 gene as frequent, which 11 (8), C2 (4), A4(1), A5(1) genes. Conclusion is first report prevalence Egypt. All these multidrug-resistant tested Close monitoring such mandatory prevent widespread dissemination code for antibiotic resistance.

Язык: Английский

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Investigation of multidrug-resistant plasmids from carbapenemase-producing Klebsiella pneumoniae clinical isolates from Pakistan

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Июнь 29, 2023

Objectives The study aim was to investigate multidrug-resistant (MDR) plasmids from a collection of 10 carbapenemase-producing Klebsiella pneumoniae clinical isolates identified within the same healthcare institution in Pakistan. Full characterization MDR including structure, typing characteristics, and AMR content as well determination their plasmid-based antimicrobial susceptibility profiles were carried out. Methods Plasmids isolated , corresponding set Escherichia coli transconjugants, then sequenced using Nanopore/Illumina technology generate plasmid hybrid assemblies. plasmids, next generation sequencing (NGS)-based profiles, incompatibility groups, types, structure analyzed Galileo platform. For E. NGS-based compared NGS-predicted phenotypes conventional broth microdilution (BMD) testing (AST) results. Results All K. (carrying either bla NDM-1 or/and OXA-48 ) multiple encoding 34 resistance genes (ARGs) conferring antimicrobials 6 different classes. groups types were: IncC (types 1 3), IncFIA (type 26) IncFIB, IncFII K1, K2, K7, K9), IncHI1B, IncL. None ESBL -plasmids this previously described. Most NDM-1- shared identical regions suggesting potential genetic material/plasmid exchange between collection. majority transconjugants correlated with both predicted AST Conclusion This highlights complexity diversity background emphasizes need for determine complete molecular monitor through transmission multi-resistant bacterial pathogens.

Язык: Английский

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Novel in-genome based analysis demonstrates the evolution of OmpK37, antimicrobial resistance gene from a potentially pathogenic pandrug resistant Klebsiella pneumoniae MS1 isolated from healthy broiler feces

The Science of The Total Environment, Год журнала: 2024, Номер 930, С. 172713 - 172713

Опубликована: Апрель 23, 2024

Язык: Английский

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Occurrence of Pantoea agglomerans bloodstream infection in neonatal intensive care unit at tertiary hospital in Tanzania: antibiotic susceptibility profile and clinical outcome

Bulletin of the National Research Centre/Bulletin of the National Research Center, Год журнала: 2024, Номер 48(1)

Опубликована: Май 13, 2024

Abstract Background Pantoea agglomerans (P. agglomerans) is an environmental gram-negative bacterium that rarely infects humans. P. infections have never been reported in Tanzania. We investigated the occurrence of bloodstream among neonates Intensive Care Unit (NICU) and their subsequent clinical outcome occurred 2019. Methodology Blood samples were collected from with sepsis. A total 19 isolated 17 infected neonates; two had twice. 14 patient files retrieved medical records. Results The mean age 3.75 ± 7.95 days. Isolated showed high sensitivity to antibiotics particularly chloramphenicol (94.7%), piperacillin-tazobactam (94.7%) meropenem (94.7%). mortality rate was 71.4% 35.7% dying before Antibiotic Susceptibility Test results for appropriate management. Infection Prevention Control (IPC) team shut NICU thorough decontamination which helped stop occurrence. Conclusions at uncommon aetiology pathogen neonatal sepsis associated rates despite multiple antibiotics. This calls strengthening infection control measures introduction surveillance pathogens capable causing human infections.

Язык: Английский

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Prevalence and Antibiotic Resistance Pattern of Streptococcus, Staphylococcus, Neisseria meningitidis and Enterobacteriaceae in Two Reference Hospitals of Yaoundé: An Overview before and during COVID-19 Pandemic Era

Antibiotics, Год журнала: 2023, Номер 12(5), С. 929 - 929

Опубликована: Май 18, 2023

The COVID-19 pandemic led to tremendously use of antimicrobial due the lack proper treatment strategies, raising concerns about emergence resistance (AMR). This study aimed at determining prevalence and antibiotic pattern selected bacteria isolates in 02 referral health facilities Yaoundé before during era. We conducted a retrospective over period 03 years (from 1 January 2019 31 December 2021) bacteriology units Central General Hospitals Yaoundé, Cameroon. Data on genera (Streptococcus, Staphylococcus, Neisseria meningitidis Enterobacteriaceae) as well their corresponding specifics antibiotics: Cefixime, azythromycin erythromycin were obtained from laboratory records. global rate correlation with antibiotics according era was determined compared. For p < 0.05, difference statistically significant. In all, 426 bacterial strains included. It appeared that highest number lowest recorded pre-COVID-19 (160 vs. 58.8% rate). Conversely, lower but greater burden (2020 amount peak registered 2020, year onset (120 70% 2020 146 58.9% 2021). contrast almost all others groups where quite constant or decreasing years, Enterobacteriaceae exhibited [60% (48/80) 86.9% (60/69) 64.5% (61/95) 2021)]. Concerning antibiotics, unlike erythromycin, related resitance increased Cefixim tends decrease (2020) re-increase one therafter. A significant association found between resistant cefixime (R = 0.7; 0.0001) also, Staphylococcus 0.8; 0.0001). These data showed herogeneous MDR time suggesting needs be more closely monitored.

Язык: Английский

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