Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(9), С. 4928 - 4938
Опубликована: Фев. 23, 2024
Sliver
nanoparticles
(AgNPs)
have
attracted
tremendous
interest
as
an
alternative
to
commercially
available
antibiotics
due
their
low
microbial
resistance
and
broad-spectrum
antimicrobial
activity.
However,
AgNPs
are
highly
reactive
unstable
susceptible
fast
oxidation.
Synthesizing
stable
efficient
using
green
chemistry
principles
remains
a
major
challenge.
To
address
this
issue,
we
establish
facile
route
form
AgNP-doped
zein
nanoparticle
core–satellite
superstructures
with
ultralow
minimum
bactericidal
concentration
(MBC).
In
brief,
polyphenol
surface-functionalization
of
was
performed,
the
epigallocatechin
gallate
(EGCG)
layer
on
served
reducing-cum-stabilizing
agent.
We
used
EGCG-decorated
(ZE)
template
direct
nucleation
growth
develop
metallized
hybrid
(ZE-Ag).
The
monodispersed
(∼150
nm)
decorated
∼4.9
nm
were
synthesized
successfully.
spatial
restriction
EGCG
by
confined
only
surface
particles,
which
prevented
formation
entangled
clusters
polyphenols
concomitantly
inhibited
coalescence
oxidation
AgNPs.
Thus,
strategy
improved
effective
specific
area
AgNPs,
result,
ZE-Ag
efficiently
killed
indicator
bacteria,
Escherichia
coli
(E.
coli)
Methicillin-resistant
Staphylococcus
aureus(MRSA)
after
20
min
incubation,
MBCs
2
4
μg/mL,
respectively.
This
situation
indicated
that
as-prepared
possessed
potent
short-term
sterilization
capability.
Moreover,
simulated
wound
infection
model
also
confirmed
promising
application
composite.
work
provides
new
insights
into
synthesis
emerging
in
food
preservation,
packaging,
biomedicine,
catalysis.
Abstract
Background
Novel
platforms
using
nanotechnology-based
medicines
have
exponentially
increased
in
our
daily
lives.
The
unique
characteristics
of
metal
oxide
and
noble
metals
nanoparticles
make
them
suitable
for
different
fields
including
antimicrobial
agents,
cosmetics,
textiles,
wound
dressings,
anticancer
drug
carriers.
Methods
This
study
focuses
on
the
biosynthesis
small-sized
SNPs
exo-metabolites
Fusarium
oxysporum
via
bioprocess
optimization
Plackett-Burman
(PBD)
central
composite
designs
(CCD)
while
evaluating
their
multifaceted
bioactivities.
Results
successful
biofabrication
smaller-sized
with
an
average
particle
size
~
5
nm
was
achieved
upon
optimization.
developed
exhibited
significant
antibacterial
activity
against
multidrug-resistant
bacterial
pathogens
a
concentration-
time-dependent
manner.
minimum
inhibitory
concentrations
(MICs)
were
0.078
µg/ml
(
Escherichia
coli
),
0.156
Pseudomonas
aeruginosa
1.25
Enterococcus
faecalis
bactericidal
(MBCs)
correspondingly
µg/ml,
0.312
µg/l,
µg/ml.
SNPs-treated
cells
displayed
bacteriostatic
effects
as
revealed
by
time-kill
assay
ultrastructure
changes
observed
SEM
TEM
analyses.
results
marked
potent
antioxidant
DPPH,
O
2
•−
,
H
OH-radicals
IC
50
values
74.3,
96.7,
116.6,
167.9
respectively.
Significantly,
biosynthesized
cytotoxic
MCF-7,
A549,
HepG-2
cell
lines
89.4,
121.4,
138.9
promising
photocatalytic
efficiency
at
times
compared
dark
conditions.
highest
decolorization
percentage
crystal
violet
dye
98.60%
after
240
min
100
µg
concentration.
Conclusions
green
synthesis
F.
exometabolites
is
eco-friendly,
inexpensive,
production
small-size,
greatly
stabilized
nanoparticles.
corroborated
that
can
be
highly
enough
to
applied
control
systems,
ameliorating
free
radical-related
disorders,
photocatalyst
wastewater
treatment.
Green Processing and Synthesis,
Год журнала:
2025,
Номер
14(1)
Опубликована: Янв. 1, 2025
Abstract
In
this
study,
Ag@Cu
alloy
nanoparticles
and
silver
nanowires
(AgNWs)
were
synthesized
by
a
green
method
using
the
Pterospermum
heterophyllum
extract.
To
study
influence
of
precursor
ratio
on
synthesis
Ag@Cu,
molar
Ag
Cu
was
changed
to
10:7,
10:6,
10:5,
10:4.
concentration
formation
AgNWs,
AgNO
3
varied
with
values
such
as
3,
4,
5,
6,
7,
8
mM.
The
results
showed
that
spherical
formed
uniformly
when
Ag:Cu
high.
branched
structures
appeared
10:6
10:7.
AgNWs
strongly
depended
concentration,
similar
polyol
method.
5
mM
most
suitable
for
AgNWs.
have
been
studied
surface-enhanced
Raman
scattering
effects
MB
dye.
both
types
particles
could
enhance
enhancement
factors
up
10
9
.
This
proved
products
equivalent
applications
chemical
methods.
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(9), С. 4928 - 4938
Опубликована: Фев. 23, 2024
Sliver
nanoparticles
(AgNPs)
have
attracted
tremendous
interest
as
an
alternative
to
commercially
available
antibiotics
due
their
low
microbial
resistance
and
broad-spectrum
antimicrobial
activity.
However,
AgNPs
are
highly
reactive
unstable
susceptible
fast
oxidation.
Synthesizing
stable
efficient
using
green
chemistry
principles
remains
a
major
challenge.
To
address
this
issue,
we
establish
facile
route
form
AgNP-doped
zein
nanoparticle
core–satellite
superstructures
with
ultralow
minimum
bactericidal
concentration
(MBC).
In
brief,
polyphenol
surface-functionalization
of
was
performed,
the
epigallocatechin
gallate
(EGCG)
layer
on
served
reducing-cum-stabilizing
agent.
We
used
EGCG-decorated
(ZE)
template
direct
nucleation
growth
develop
metallized
hybrid
(ZE-Ag).
The
monodispersed
(∼150
nm)
decorated
∼4.9
nm
were
synthesized
successfully.
spatial
restriction
EGCG
by
confined
only
surface
particles,
which
prevented
formation
entangled
clusters
polyphenols
concomitantly
inhibited
coalescence
oxidation
AgNPs.
Thus,
strategy
improved
effective
specific
area
AgNPs,
result,
ZE-Ag
efficiently
killed
indicator
bacteria,
Escherichia
coli
(E.
coli)
Methicillin-resistant
Staphylococcus
aureus(MRSA)
after
20
min
incubation,
MBCs
2
4
μg/mL,
respectively.
This
situation
indicated
that
as-prepared
possessed
potent
short-term
sterilization
capability.
Moreover,
simulated
wound
infection
model
also
confirmed
promising
application
composite.
work
provides
new
insights
into
synthesis
emerging
in
food
preservation,
packaging,
biomedicine,
catalysis.