International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12774 - 12774
Опубликована: Ноя. 28, 2024
4-aminopyridine
(4-AP)
is
a
non-selective
blocker
of
voltage-dependent
K+
channels
used
to
improve
walking
in
multiple
sclerosis
patients,
and
it
may
be
useful
the
treatment
cerebellar
diseases.
In
animal
models,
4-AP
as
convulsant
agent.
When
administered
intrahippocampally,
induces
acute
local
glucose
hypermetabolism
significant
brain
damage,
while
i.p.
administration
causes
less
neuronal
damage.
This
study
aimed
investigate
effects
single
on
metabolism
well
viability
signs
neuroinflammation
3
days
after
insult.
Brain
was
evaluated
by
[18F]FDG
PET
neuroimaging.
uptake
analyzed
based
volumes
interest
(VOIs)
voxel-based
(SPM)
analyses.
The
results
showed
that
independently
type
data
analysis
(VOIs
or
SPM),
induced
generalized
hypometabolism,
except
cerebellum.
Furthermore,
SPM
normalized
whole
revealed
hypermetabolism.
neurohistochemical
assays
hippocampal
astrocyte
reactivity
insult,
without
inducing
changes
integrity
microglia-mediated
neuroinflammation.
Thus,
metabolic
neuroinflammatory
profiles
response
clearly
differed
from
reported
for
intrahippocampal
administration.
Finally,
suggest
cerebellum
might
more
resilient
4-AP-induced
hypometabolism.
Tomography,
Год журнала:
2023,
Номер
9(2), С. 567 - 578
Опубликована: Март 4, 2023
We
have
integrated
a
compact
and
lightweight
PET
with
an
existing
CT
image-guided
small
animal
irradiator
to
enable
practical
onboard
PET/CT
preclinical
radiation
therapy
(RT)
research.
The
stationary
full-ring
detectors
has
~1.1
mm
uniform
spatial
resolution
over
its
imaging
field-of-view
of
8.0
cm
diameter
3.5
axial
length
was
mechanically
installed
inside
the
in
tandem
configuration
unit.
A
common
bed
used
for
acquiring
sequential
dual
functional
anatomical
images
independent
control
acquisition
systems.
reconstructed
were
co-registered
based
on
standard
multi-modality
image
calibration
registration
processes.
Phantom
studies
conducted
evaluate
system
performance.
measured
mean
error
~0.3
mm.
With
one-bed
three-bed
acquisitions,
initial
tumor
focused
whole-body
[18F]FDG
acquired
test
capability
guidance
RT
Overall,
results
shown
that
PET/CT/RT
can
provide
advantageous
significantly
enhance
accuracy
delineation
targeting
should
new
potentially
breakthrough
research
applications.
The European Physical Journal Plus,
Год журнала:
2023,
Номер
138(12)
Опубликована: Дек. 10, 2023
Abstract
The
production
of
medical
radionuclides
is
one
the
research
activities
carried
out
in
framework
SPES
(Selective
Production
Exotic
Species)
project
under
completion
stage
at
Legnaro
National
Laboratories
Institute
for
Nuclear
Physics
(INFN-LNL).
heart
70-MeV
proton
cyclotron
having
a
dual-beam
extraction,
installed
and
commissioned
new
building
equipped
with
ancillary
laboratories
currently
construction.
main
goal
realization
an
advanced
ISOL
(Isotope
Separation
On-Line)
facility
to
produce
re-accelerated
exotic
ion
beams
fundamental
nuclear
physics
studies.
double-beam
extraction
system
allows
simultaneously
carry
applied
research,
such
as
medicine
(SPES-
$$\gamma$$
γ
).
This
paper
summarizes
results
obtained
interdisciplinary
projects
LARAMED
(LAboratory
RAdionuclides
MEDicine)
ISOLPHARM
(ISOL
technique
radioPHARMaceuticals).
first
one,
based
upon
direct
activation
method,
focused
on
spotlight
international
community
(e.g.,
$$^{99m}$$
99m
Tc,
$$^{67}$$
67
Cu,
$$^{52/51}$$
52/51
Mn,
$$^{47}$$
47
Sc
Tb
isotopes),
from
cross-section
measurements
up
preclinical
other
exploits
development
radioisotopes
high-specific
activity,
$$^{111}$$
111
Ag,
going
beyond
state
art
field.
most
recent
SPES-
future
perspective
are
here
described,
characterized
by
consolidated
network
collaborations
national
institutions.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12774 - 12774
Опубликована: Ноя. 28, 2024
4-aminopyridine
(4-AP)
is
a
non-selective
blocker
of
voltage-dependent
K+
channels
used
to
improve
walking
in
multiple
sclerosis
patients,
and
it
may
be
useful
the
treatment
cerebellar
diseases.
In
animal
models,
4-AP
as
convulsant
agent.
When
administered
intrahippocampally,
induces
acute
local
glucose
hypermetabolism
significant
brain
damage,
while
i.p.
administration
causes
less
neuronal
damage.
This
study
aimed
investigate
effects
single
on
metabolism
well
viability
signs
neuroinflammation
3
days
after
insult.
Brain
was
evaluated
by
[18F]FDG
PET
neuroimaging.
uptake
analyzed
based
volumes
interest
(VOIs)
voxel-based
(SPM)
analyses.
The
results
showed
that
independently
type
data
analysis
(VOIs
or
SPM),
induced
generalized
hypometabolism,
except
cerebellum.
Furthermore,
SPM
normalized
whole
revealed
hypermetabolism.
neurohistochemical
assays
hippocampal
astrocyte
reactivity
insult,
without
inducing
changes
integrity
microglia-mediated
neuroinflammation.
Thus,
metabolic
neuroinflammatory
profiles
response
clearly
differed
from
reported
for
intrahippocampal
administration.
Finally,
suggest
cerebellum
might
more
resilient
4-AP-induced
hypometabolism.
