Comparative transcriptomics reveal contrasting fungal strategies in a plant pathogen versus an endophyte during initial host colonization DOI Open Access
Soumya Moonjely, Frances Trail

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

ABSTRACT Conidial germination marks the beginning of fungal life cycle on host plant, leading to disease or mutually beneficial relationships. Here, we use comparative transcriptomics unravel transcriptional similarities and differences during conidial initial colony establishment plant pathogen Fusarium graminearum, endophyte Metarhizium anisopliae . Our comparison crosses four stages from fresh conidia polar growth, hyphal extension, ending in either first branching (on medium) appressorium formation barley). F. graminearum exhibited a higher number upregulated genes for CAZymes, specialized metabolites effectors compared M. interaction with host, particularly stage, reflecting its pathogenic nature. The appressoria by barley roots has not been documented previously includes both morphological characteristics gene expression patterns that regulate development. analysis reveals reduced transcript levels CAZyme metabolite , less aggressive penetration approach. candidate associated indole-3-acetic acid synthesis were supporting endophytic lifestyle, suggesting fungus uses phytohormone based strategy interact hosts. Collectively, our findings expand transcriptome resources provide valuable insights into networks involved initiation infection versus fungi, as well documenting time, IMPORTANCE is step colonization diverse environments. Here examine two species belongs Order Hypocreales distinct lifestyles. causal agent head blight cereal crops, whereas an insect root which forms associations plants. We profiles these under nutrient conditions across germination. study shows profile encoding carbohydrate-active enzymes, metabolites, putative varies between results this spore fungus.

Язык: Английский

Genome-Wide Identification and Functional Characterization of the Acyl-CoA Dehydrogenase (ACAD) Family in Fusarium sacchari DOI Open Access
Quan Zeng,

Quan Yu,

Yingxi Mo

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 973 - 973

Опубликована: Янв. 24, 2025

Fusarium sacchari is one of the primary causal agents Pokkah boeng disease (PBD), an important sugarcane worldwide. The acyl-CoA dehydrogenases (ACADs) constitute a family flavoenzymes involved in β-oxidation fatty acids and amino acid catabolism mitochondria. However, role ACADs pathogenesis F. unclear. Here, 14 ACAD-encoding genes (FsACAD-1–FsACAD-14) were identified by screening entire genome sequence sacchari. FsACAD are distributed across seven chromosomes classified into clades based on phylogenetic analysis protein sequences. In vivo mRNA quantification revealed that differentially expressed during infection, their expression patterns differ significantly response to vitro induction different classes. Fatty utilization assays FsACAD-deletion mutants FsACADs varied preference ability break down acids. There was variation adverse impact fungal traits, including growth, conidiation, stress tolerance, virulence. These findings provide insights roles sacchari, identification offers potential new targets for improved control PBD.

Язык: Английский

Процитировано

0
Fusarium sacchari Effector FsMEP1 Contributes to Virulence by Disturbing Localization of Thiamine Thiazole Synthase ScTHI2 from Sugarcane DOI Open Access
Lulu Wang, Deng Wu,

Tianshu Hong

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(22), С. 12075 - 12075

Опубликована: Ноя. 10, 2024

is a significant pathogenic fungus that causes sugarcane Pokkah Boeng. Proteins secreted by fungi can be delivered into hosts to suppress plant immunity and establish infection. However, there still much discovered regarding

Язык: Английский

Процитировано

0
Comprehensive Genomic and Proteomic Analysis Identifies Effectors of Fusarium oxysporum f. sp. melongenae DOI Creative Commons

Jiong Wu,

Pengfei Wang,

Wuhong Wang

и другие.

Journal of Fungi, Год журнала: 2024, Номер 10(12), С. 828 - 828

Опубликована: Ноя. 28, 2024

wilt in eggplant caused by

Язык: Английский

Процитировано

0
Comparative transcriptomics reveal contrasting fungal strategies in a plant pathogen versus an endophyte during initial host colonization DOI Open Access
Soumya Moonjely, Frances Trail

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

ABSTRACT Conidial germination marks the beginning of fungal life cycle on host plant, leading to disease or mutually beneficial relationships. Here, we use comparative transcriptomics unravel transcriptional similarities and differences during conidial initial colony establishment plant pathogen Fusarium graminearum, endophyte Metarhizium anisopliae . Our comparison crosses four stages from fresh conidia polar growth, hyphal extension, ending in either first branching (on medium) appressorium formation barley). F. graminearum exhibited a higher number upregulated genes for CAZymes, specialized metabolites effectors compared M. interaction with host, particularly stage, reflecting its pathogenic nature. The appressoria by barley roots has not been documented previously includes both morphological characteristics gene expression patterns that regulate development. analysis reveals reduced transcript levels CAZyme metabolite , less aggressive penetration approach. candidate associated indole-3-acetic acid synthesis were supporting endophytic lifestyle, suggesting fungus uses phytohormone based strategy interact hosts. Collectively, our findings expand transcriptome resources provide valuable insights into networks involved initiation infection versus fungi, as well documenting time, IMPORTANCE is step colonization diverse environments. Here examine two species belongs Order Hypocreales distinct lifestyles. causal agent head blight cereal crops, whereas an insect root which forms associations plants. We profiles these under nutrient conditions across germination. study shows profile encoding carbohydrate-active enzymes, metabolites, putative varies between results this spore fungus.

Язык: Английский

Процитировано

0