Whole-Genome Sequencing of Flammulina filiformis and Multi-Omics Analysis in Response to Low Temperature DOI Creative Commons
Xuanqiang Liang, Jing Han,

Yuqin Cui

и другие.

Journal of Fungi, Год журнала: 2025, Номер 11(3), С. 229 - 229

Опубликована: Март 17, 2025

The growth of Flammulina filiformis is strongly dependent on low-temperature cues for the initiation primordia formation. To obtain a comprehensive understanding molecular mechanisms that govern mycelial response to cold stress, de novo genome sequencing F. monokaryon and multi-omics data (transcriptome metabolome) analyses mycelia, primordia, fruiting bodies were conducted in present study. Genome based PacBio HiFi Hi-C resulted 36.3 Mb sequence mapped 12 chromosomes, comprising 11,886 protein-coding genes. A total 25 cold-responsive (COR) genes 520 cold-adapted enzymes identified genome. Multi-omics showed pathways related carbohydrate metabolism mycelia under low temperature (10 °C) significantly enriched. Further examination expression profiles carbohydrate-active (CAZymes) involved revealed out 515 CAZyme filiformis, 58 specifically upregulated conditions. By contrast, levels these reverted those prior exposure. These indicate are important adaptation filiformis. This research contributes targeted breeding

Язык: Английский

Whole-Genome Sequencing of Flammulina filiformis and Multi-Omics Analysis in Response to Low Temperature DOI Creative Commons
Xuanqiang Liang, Jing Han,

Yuqin Cui

и другие.

Journal of Fungi, Год журнала: 2025, Номер 11(3), С. 229 - 229

Опубликована: Март 17, 2025

The growth of Flammulina filiformis is strongly dependent on low-temperature cues for the initiation primordia formation. To obtain a comprehensive understanding molecular mechanisms that govern mycelial response to cold stress, de novo genome sequencing F. monokaryon and multi-omics data (transcriptome metabolome) analyses mycelia, primordia, fruiting bodies were conducted in present study. Genome based PacBio HiFi Hi-C resulted 36.3 Mb sequence mapped 12 chromosomes, comprising 11,886 protein-coding genes. A total 25 cold-responsive (COR) genes 520 cold-adapted enzymes identified genome. Multi-omics showed pathways related carbohydrate metabolism mycelia under low temperature (10 °C) significantly enriched. Further examination expression profiles carbohydrate-active (CAZymes) involved revealed out 515 CAZyme filiformis, 58 specifically upregulated conditions. By contrast, levels these reverted those prior exposure. These indicate are important adaptation filiformis. This research contributes targeted breeding

Язык: Английский

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