The Identification of Key Genes and Biological Pathways in Cardiac Arrest by Integrated Bioinformatics and Next Generation Sequencing Data Analysis DOI Open Access
Basavaraj Vastrad, Chanabasayya Vastrad

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 19, 2024

Abstract Cardiac arrest (CA) is a common cause of death world wide. The disease has lacks effective treatment. Efforts have been made to elucidate the molecular pathogenesis CA, but mechanisms remain elusive. To identify key genes and pathways in next generation sequencing (NGS) GSE200117 dataset was downloaded from Gene Expression Omnibus (GEO) database. DESeq2 tool used recognize differentially expressed (DEGs). ontology (GO) REACTOME pathway enrichment analyses were performed analyze DEGs associated signal g:Profiler IID database construct protein-protein interaction (PPI) network, modules analysis using Cytoscape. A miRNA-hub gene regulatory network TF-hub then constructed screen miRNAs, TFs hub by miRNet NetworkAnalyst Cityscape software. Receiver operating characteristic curve (ROC) verified genes. In total, 844 identified, comprising 414 up regulated 430 down GO indicated that for CA mainly enriched organonitrogen compound metabolic process, response stimulus, translation immune system. Ten (up-regulated: HSPA8, HOXA1, INCA1 TP53; down-regulated: HSPB1, LMNA, SNCA, ADAMTSL4 PDLIM7) screened. We also predicted miRNAs (hsa-mir-1914-5p hsa-mir-598-3p) (JUN PRRX2) targeting This study uses series bioinformatics technologies obtain hug genes, TFs, related CA. These results provide us with new ideas finding biomarkers treatment methods

Язык: Английский

BDNF/BDNF-AS Gene Polymorphisms Modulate Treatment Response and Remission in Bipolar Disorder: A Randomized Clinical Trial DOI Open Access

Anton Shkundin,

Heather E. Wheeler, James Sinacore

и другие.

Journal of Personalized Medicine, Год журнала: 2025, Номер 15(2), С. 62 - 62

Опубликована: Фев. 7, 2025

Background: Bipolar disorder (BD) is a chronic condition associated with treatment resistance, cognitive decline, structural brain changes, and an approximately 13-year reduction in life expectancy compared to the general population. Depression BD substantially impairs quality of life, while neuroinflammation excitotoxicity are thought contribute recurrence mood episodes disease progression. Brain-derived neurotrophic factor (BDNF) plays key role neuronal growth function, its dysregulation being linked various psychiatric disorders. This study extension previously published clinical trial was conducted assess effects three BDNF BDNF-AS gene polymorphisms (rs1519480, rs6265, rs10835210) on outcomes serum levels patients treatment-resistant bipolar depression (TRBDD) over eight-week period. Methods: included 41 participants from randomized trial, all whom had available samples genotype data. The participants, aged 21 65, were diagnosed disorder, assessed using Maudsley Staging Method. Participants randomly assigned receive either escitalopram plus placebo (ESC+PBO) or celecoxib (ESC+CBX) 8-week Statistical analyses mixed ANOVA chi-square tests compare minor allele carrier status SNPs response remission rates. Results: Non-carriers rs6265 A (p = 0.005) carriers rs10835210 0.007) showed significantly higher adjunctive alone. Additionally, rates after both non-carriers across However, notably rs1519480 G allele, as well allele. Conclusions: suggests that genetic variations genes influence disorder.

Язык: Английский

Процитировано

0

Mitochondrially Transcribed dsRNA Mediates Manganese-induced Neuroinflammation DOI Creative Commons
Avanti Gokhale, Hadassah Mendez-Vazquez, Maureen M. Sampson

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 20, 2025

Abstract Manganese (Mn) is an essential trace element required for various biological functions, but excessive Mn levels are neurotoxic and lead to significant health concerns. The mechanisms underlying Mn-induced neurotoxicity remain poorly understood. Neuropathological studies of affected brain regions reveal astrogliosis, neuronal loss, along with evidence neuroinflammation. Here, we present a novel Mn-dependent mechanism linking mitochondrial dysfunction We found that disrupts transcriptome processing, resulting in the accumulation complementary RNAs form double-stranded RNA (dsRNA). This dsRNA released cytoplasm, where it activates cytosolic sensor pathways, triggering type I interferon responses inflammatory cytokine production. 100-day human cerebral organoids, observed predominantly mature astrocytes. Similar effects were vivo mouse model carrying mutations SLC30A10 gene, which results accumulation. These findings highlight previously unrecognized role neuroinflammation provide insights into molecular basis manganism. propose this dsRNA-induced pathway has broad implications neurodegenerative diseases caused by environmental or genetic insults.

Язык: Английский

Процитировано

0

Targeting CXCR2 signaling in inflammatory lung diseases: neutrophil-driven inflammation and emerging therapies DOI
Md Sadique Hussain, Ahsas Goyal,

Kavita Goyal

и другие.

Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2025, Номер unknown

Опубликована: Март 6, 2025

Язык: Английский

Процитировано

0

Brain-derived neurotrophic factor and stress perception DOI

Anton Shkundin,

Heather E. Wheeler, James Sinacore

и другие.

Personalized Medicine in Psychiatry, Год журнала: 2024, Номер 47-48, С. 100139 - 100139

Опубликована: Окт. 15, 2024

Язык: Английский

Процитировано

1

Hemozoin induces malaria via activation of DNA damage, p38 MAPK and neurodegenerative pathways in a human iPSC-derived neuronal model of cerebral malaria DOI Creative Commons

Abida lslam Pranty,

Leon-Phillip Szepanowski, Wasco Wruck

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Окт. 23, 2024

Abstract Malaria caused by Plasmodium falciparum infection results in severe complications including cerebral malaria (CM), which approximately 30% of patients end up with neurological sequelae. Sparse vitro cell culture-based experimental models recapitulate the molecular basis CM humans has impeded progress our understanding its etiology. This study employed healthy human induced pluripotent stem cells (iPSCs)-derived neuronal cultures stimulated hemozoin (HMZ) - malarial toxin as a model for CM. Secretome, qRT-PCR, Metascape, and KEGG pathway analyses were conducted to assess elevated proteins, genes, pathways. Neuronal treated HMZ showed enhanced secretion interferon-gamma (IFN-γ), interleukin (IL)1-beta (IL-1β), IL-8 IL-16. Enrichment analysis revealed malaria, positive regulation cytokine production mitogen-activated protein kinase (MAPK) cascade confirm inflammatory response exposure. assessment up-regulation MAPK neurodegenerative diseases-associated pathways corroborates findings from previous studies. Additionally, DNA damage neurons. unveiled that exposure HMZ, activates molecules similar those observed diseases. Furthermore, is an alternative rodent

Язык: Английский

Процитировано

1

BDNF blood levels as a potential biomarker Predictor of treatment response and remission in bipolar depression DOI

Anton Shkundin,

James Sinacore,

Angelos Halaris

и другие.

Personalized Medicine in Psychiatry, Год журнала: 2024, Номер 47-48, С. 100144 - 100144

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

1

The Identification of Key Genes and Biological Pathways in Cardiac Arrest by Integrated Bioinformatics and Next Generation Sequencing Data Analysis DOI Open Access
Basavaraj Vastrad, Chanabasayya Vastrad

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 19, 2024

Abstract Cardiac arrest (CA) is a common cause of death world wide. The disease has lacks effective treatment. Efforts have been made to elucidate the molecular pathogenesis CA, but mechanisms remain elusive. To identify key genes and pathways in next generation sequencing (NGS) GSE200117 dataset was downloaded from Gene Expression Omnibus (GEO) database. DESeq2 tool used recognize differentially expressed (DEGs). ontology (GO) REACTOME pathway enrichment analyses were performed analyze DEGs associated signal g:Profiler IID database construct protein-protein interaction (PPI) network, modules analysis using Cytoscape. A miRNA-hub gene regulatory network TF-hub then constructed screen miRNAs, TFs hub by miRNet NetworkAnalyst Cityscape software. Receiver operating characteristic curve (ROC) verified genes. In total, 844 identified, comprising 414 up regulated 430 down GO indicated that for CA mainly enriched organonitrogen compound metabolic process, response stimulus, translation immune system. Ten (up-regulated: HSPA8, HOXA1, INCA1 TP53; down-regulated: HSPB1, LMNA, SNCA, ADAMTSL4 PDLIM7) screened. We also predicted miRNAs (hsa-mir-1914-5p hsa-mir-598-3p) (JUN PRRX2) targeting This study uses series bioinformatics technologies obtain hug genes, TFs, related CA. These results provide us with new ideas finding biomarkers treatment methods

Язык: Английский

Процитировано

0