Limbal stem cell deficiency approaches and limbal niche restoration DOI Creative Commons

Zahra Bibak-Bejandi,

Zohre Arabpour,

Alireza Razavi

и другие.

Indian Journal of Ophthalmology, Год журнала: 2025, Номер 73(4), С. 468 - 482

Опубликована: Март 27, 2025

Approaches to limbal stem cell deficiency remain challenging, especially in bilateral cases, where healthy cells are not accessible. While living-related allogeneic and sources have been utilized, their dependence on immunosuppression its associated side effects pose significant limitations. Mucosal mesenchymal shown potential for differentiation into promoting corneal healing, primarily when cultured the amniotic membrane or fibrin. However, none can fully replicate original limbus. Innovations surgical techniques, such as simple oral mucosal transplantation subconjunctival intrastromal injections, emerging approaches. For successful regeneration, both appropriate suitable scaffolds essential. Recent studies decellularized acellularized limbus models demonstrated provide a three-dimensional native structure seeding, retention, differentiation. Creating thin, evenly scaffold is critical step ensuring proper corneo-limbal slope formation, facilitating migration ocular surface. Harvesting limbus, decellularization, seeding three main steps reconstruction. focus microkeratome-assisted harvesting create even, 360-degree graft. This technique helps form an attached interface, migration. In second step, acellularization performed preserve extracellular matrix much possible, maintaining hemostasis supporting paracrine interactions. The final involve recellularization onto eye. We summarize various decellularization methods, outcomes, More clinical needed validate this phase of treatment.

Язык: Английский

Limbal stem cell deficiency approaches and limbal niche restoration DOI Creative Commons

Zahra Bibak-Bejandi,

Zohre Arabpour,

Alireza Razavi

и другие.

Indian Journal of Ophthalmology, Год журнала: 2025, Номер 73(4), С. 468 - 482

Опубликована: Март 27, 2025

Approaches to limbal stem cell deficiency remain challenging, especially in bilateral cases, where healthy cells are not accessible. While living-related allogeneic and sources have been utilized, their dependence on immunosuppression its associated side effects pose significant limitations. Mucosal mesenchymal shown potential for differentiation into promoting corneal healing, primarily when cultured the amniotic membrane or fibrin. However, none can fully replicate original limbus. Innovations surgical techniques, such as simple oral mucosal transplantation subconjunctival intrastromal injections, emerging approaches. For successful regeneration, both appropriate suitable scaffolds essential. Recent studies decellularized acellularized limbus models demonstrated provide a three-dimensional native structure seeding, retention, differentiation. Creating thin, evenly scaffold is critical step ensuring proper corneo-limbal slope formation, facilitating migration ocular surface. Harvesting limbus, decellularization, seeding three main steps reconstruction. focus microkeratome-assisted harvesting create even, 360-degree graft. This technique helps form an attached interface, migration. In second step, acellularization performed preserve extracellular matrix much possible, maintaining hemostasis supporting paracrine interactions. The final involve recellularization onto eye. We summarize various decellularization methods, outcomes, More clinical needed validate this phase of treatment.

Язык: Английский

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