Natural-based solutions to mitigate dietary microplastics side effects in fish

Chemosphere, Год журнала: 2024, Номер unknown, С. 143587 - 143587

Опубликована: Окт. 1, 2024

Язык: Английский

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Tissue factor pathway inhibitors disrupt structures of rhabdovirus/ranairidovirus and inhibit viral infection in Chinese perch, Siniperca chuatsi

Fish & Shellfish Immunology, Год журнала: 2024, Номер 149, С. 109553 - 109553

Опубликована: Апрель 13, 2024

Язык: Английский

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Unveiling novel scallop-derived antimicrobial peptides targeting host-associated vibrios for sustainable pathogen control in aquaculture

Aquaculture, Год журнала: 2024, Номер 592, С. 741238 - 741238

Опубликована: Июнь 18, 2024

Язык: Английский

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Molecular Characterization, Antibacterial and Immunoregulatory Activities of <i>Liver-Expressed Antimicrobial Peptide 2 (LEAP-2)</i> in Black Rockfish, <i>Sebastes Schlegelii</i>

Опубликована: Янв. 1, 2024

LEAP2 (liver expression antimicrobial peptide 2), is an widely found in vertebrates and mainly expressed liver. plays a vital role host innate immunity. In teleosts, number of homologs have been reported, but their vivo effects on defense are still limited. this study, homolog (SsLEAP2) was identified from black rockfish, Sebastes schlegelii, its structure, biological functions were analyzed. The results showed that the open reading frame SsLEAP2 300 bp, with 5’- untranslated region (UTR) 375 bp 3’ - UTR 238 bp. deduced amino acid sequence shares highest overall identity (96.97%) umbrosus. possesses conserved features, including signal sequence, prodomain mature peptide, which four well-conserved cysteines formed two intrachain disulphide domain. liver could be induced by experimental infection Listonella anguillarum, Edwardsiealla piscicida Rock bream iridovirus C1 (RBIV-C1). Recombinant (rSsLEAP2) purified Escherichia coli able to bind various Gram-positive Gram-negative bacteria. Further analysis rSsLEAP2 enhance respiratory burst activity, induce immune genes macrophages; as well promote proliferation chemotactic peripheral blood lymphocytes (PBLs). experiments indicated overexpression inhibit bacterial infection, increase level genes; conversely, knock down promoted decreased genes. Taken together, these suggest novel apparent antibacterial activity immunoregulatory property, thus critical against pathogens invasion.

Язык: Английский

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Synthetic Antimicrobial Peptides Fail to Induce Leucocyte Innate Immune Functions but Elicit Opposing Transcriptomic Profiles in European Sea Bass and Gilthead Seabream

Marine Drugs, Год журнала: 2024, Номер 22(2), С. 86 - 86

Опубликована: Фев. 14, 2024

Antimicrobial peptides (AMPs) are promising molecules in diverse fields, including aquaculture. AMPs possess lytic effects on a wide range of pathogens, resulting potential replacement for traditional antimicrobials In addition, they also have modulatory host immune responses. Thus, the objective this work was to evaluate immunomodulatory capability three known synthetic derived from European sea bass, NK-lysin (Nkl), hepcidin (Hamp), and dicentracin (Dic), head-kidney cell suspensions bass gilthead seabream. The tested were neither cytotoxic nor seabream cells failed modulate respiratory burst phagocytosis activities. However, modified pattern transcription immune-related genes differently both species. Peptides able promote expression marker anti-inflammatory (il10), antiviral (mx, irf3), cell-mediated cytotoxicity (nccrp1, gzmb), antibody responses (ighm) with Nkl peptide being most effective. Contrary this, those mainly resulted suppression To conclude, bass-derived can be postulated as tools immunostimulation fish farms, but more efforts required their universal use other

Язык: Английский

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Oral Administration of Antimicrobial Peptide NZ2114 Through the Microalgal Bait Tetraselmis subcordiformis (Wille) Butcher for Improving the Immunity and Gut Health in Turbot (Scophthalmus maximus L.)

Marine Biotechnology, Год журнала: 2024, Номер 26(2), С. 230 - 242

Опубликована: Март 19, 2024

Язык: Английский

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Bacillus subtilis 2118 exhibits bactericidal activity due to an inserted fish cDNA library

Aquaculture, Год журнала: 2024, Номер 593, С. 741300 - 741300

Опубликована: Июль 1, 2024

Язык: Английский

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Machine Learning-Driven Discovery and Evaluation of Antimicrobial Peptides from Crassostrea gigas Mucus Proteome

Marine Drugs, Год журнала: 2024, Номер 22(9), С. 385 - 385

Опубликована: Авг. 26, 2024

Marine antimicrobial peptides (AMPs) represent a promising source for combating infections, especially against antibiotic-resistant pathogens and traditionally challenging infections. However, traditional drug discovery methods face challenges such as time-consuming processes high costs. Therefore, leveraging machine learning techniques to expedite the of marine AMPs holds significant promise. Our study applies develop AMPs, focusing on

Язык: Английский

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Comprehensive Review of the Latest Investigations of the Health-Enhancing Effects of Selected Properties of Arthrospira and Spirulina Microalgae on Skin

Pharmaceuticals, Год журнала: 2024, Номер 17(10), С. 1321 - 1321

Опубликована: Окт. 3, 2024

and

Язык: Английский

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Boc‐Protected Phenylalanine and Tryptophan‐Based Dipeptides: A Broad Spectrum Anti‐Bacterial Agent

Biopolymers, Год журнала: 2024, Номер 116(1)

Опубликована: Дек. 24, 2024

ABSTRACT Dipeptides were constructed using hydrophobic amino acid residues following AMP prediction. After that Boc‐modification was performed on the screened peptides and finally Boc‐Phe‐Trp‐OMe Boc‐Trp‐Trp‐OMe synthesized. Even though no inhibition zones observed in agar well diffusion assays, minimum inhibitory concentration (MIC) analysis revealed anti‐bacterial activity against both Gram‐positive Gram‐negative bacteria, with MIC 90 ranging from 230 to 400 μg/mL. The crystal violet assay confirmed dipeptides' biofilm eradication disruption capabilities. Furthermore, membrane permeabilization assays indicated outer inner permeabilization, while SEM formation of fibril spherical nanostructures, likely contributing this effect. also exhibited resistance protein adsorption, non‐cytotoxicity, non‐hemolytic properties, making them promising broad‐spectrum agents potential. This study concludes Boc‐protected phenylalanine‐ tryptophan‐based dipeptides can self‐assemble be used as agents. self‐assembly these offers a versatile platform for designing biomaterials tailored properties functionalities. Research exploring potential has been limited, prompting our investigation shed light overlooked area. Our synthesized notable activity, marking significant advancement. finding suggests could emerge potent, agents, addressing urgent need effective treatments bacterial opening new avenues therapy. not only enhances understanding but highlights their innovative efficacious substantial impact clinical field.

Язык: Английский

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