Classical Monocyte Marker Gene Signatures (CMMGS) Identified by Analysis of Single-Cell and Bulk-RNA Sequencing to Predict Prognosis and Immunotherapy Response in Lung Adenocarcinoma DOI Creative Commons
Lin Han,

Yu-Han Huang,

Shencheng Ren

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Дек. 4, 2023

Abstract Background Classical monocytes, pivotal in the innate immune response, wield considerable influence over cancer prognosis and response to immunotherapy. Our aim was construct lung adenocarcinoma (LUAD) prognostic signature associated with monocytes. Methods This study encompassed 1,822 samples drawn from diverse public datasets. Initially, we conducted an comprehensive analysis of single-cell RNA sequencing data obtained Gene Expression Omnibus (GEO), identifying 145 marker genes specific classical monocyte. Univariate Cox regression Least Absolute Shrinkage Operator (LASSO) analyses were performed on TCGA training cohort a for monocyte genes. Result Patients LUAD categorized into high- low-risk groups based polygenic derived these The predictive capacity this robustly validated across distinct clinical subgroups GEO cohorts. Notably, emerged as independent indicator via multivariate analysis. Low-risk patients exhibited heightened cell infiltration, correlating inversely inflammatory activity risk score. Conversely, group demonstrated amplified abundance diversity T-cell receptor (TCR) repertoires, higher Immunophenotype scores (IPS), lower Tumor Immune Dysfunction Exclusion (TIDE) scores. Significantly, assessment three immunotherapy cohorts showcased superior immunotherapeutic among compared their high-risk counterparts. Conclusion In summary, our introduces novel rooted that proficiently prognosticates predicts patients. Supplementary Information: information is uploaded attached document.

Язык: Английский

Targeting ferroptosis: a promising approach for treating lung carcinoma DOI Creative Commons
Ziyang Wu, Tao Zhang, Wei Zhong

и другие.

Cell Death Discovery, Год журнала: 2025, Номер 11(1)

Опубликована: Янв. 29, 2025

Abstract Lung carcinoma incidence and fatality rates remain among the highest on a global scale. The efficacy of targeted therapies immunotherapies is commonly compromised by emergence drug resistance other factors, resulting in lack durable therapeutic benefits. Ferroptosis, distinct pattern cell death marked buildup iron-dependent lipid peroxides, has been shown to be novel potentially more effective treatment for lung carcinoma. However, mechanism regulatory network ferroptosis are exceptionally complex, many unanswered questions remain. In addition, research diagnosis cancer growing exponentially. Therefore, it necessary provide thorough summary latest advancements field ferroptosis. Here, we comprehensively analyze mechanisms underlying preconditions ferroptosis, defense system, associated molecular networks. potential strategies also highlighted. Targeting improves tumor enhances effectiveness drugs immunotherapies. These findings may shed fresh light management carcinoma, as well development related

Язык: Английский

Процитировано

1
Expression patterns of MCM8 in lung adenocarcinoma and its correlation with key biological processes DOI Creative Commons
Xu‐Sheng Liu,

Jin Xie,

Ruimin Wu

и другие.

European journal of medical research, Год журнала: 2025, Номер 30(1)

Опубликована: Март 3, 2025

Lung adenocarcinoma (LUAD) is one of the most common and lethal tumors. The identification diagnostic prognostic biomarkers essential to improve patient prognosis treatment outcomes. expression minichromosome maintenance complex component 8 (MCM8) in 33 cancer types was analyzed using Cancer Genome Atlas (TCGA) Genotype-Tissue Expression. Tumor normal tissues LUAD were compared TCGA data validated against four datasets from Gene Expression Omnibus. MCM8 assessed by immunohistochemistry (IHC) tissue microarrays. value Receiver Operating Characteristic curve analysis, its significance determined Kaplan–Meier analysis. CIBERSORT method used examine immune infiltration. association between m6A RNA methylation, glycolysis, ferroptosis GEPIA online tool. markedly overexpressed many tumors including LUAD. showed high accuracy for diagnosis LUAD, with an area under 0.849 dataset. overexpression tumor confirmed IHC shown be associated decreased overall survival disease-specific survival. Analysis cell infiltration that populations differed low groups. correlated genes ferroptosis. identified as a promising marker mechanism underlying effect on development response remains elucidated.

Язык: Английский

Процитировано

1
Ferroptosis in lung cancer: dual role, multi-level regulation, and new therapeutic strategies DOI Creative Commons

Yunbin Li,

Xiaosong Li, Jian Li

и другие.

Frontiers in Oncology, Год журнала: 2024, Номер 14

Опубликована: Март 7, 2024

Lung cancer is a highly prevalent malignant tumor worldwide, with high incidence and death rates. Recently, there has been increasing recognition of the role ferroptosis, unique cell mechanism, in lung cancer. This review aims to summarize current research progress on relationship between ferroptosis It also provides comprehensive analysis regulatory processes various stages, including epigenetics, transcription, post-transcription, translation, post-translation. Additionally, explores dual nature progression, which presents interesting therapeutic possibilities. On one hand, can promote escape immune surveillance reduce efficacy treatment early stages tumors. other it counter drug resistance, enhance radiosensitivity, immunotherapy. The article discusses combination strategies based mechanism ferroptosis. Overall, this offers holistic perspective onset, contribute future clinical interventions field.

Язык: Английский

Процитировано

4
Pacidusin B isolated from Phyllanthus acidus triggers ferroptotic cell death in HT1080 cells DOI Creative Commons
Guangyu Zhu,

Dian Luo,

Yueqin Zhao

и другие.

Natural Products and Bioprospecting, Год журнала: 2024, Номер 14(1)

Опубликована: Май 23, 2024

Cancer cells generally exhibit 'iron addiction' phenotypes, which contribute to their vulnerability ferroptosis inducers. Ferroptosis is a newly discovered form of programmed cell death caused by iron-dependent lipid peroxidation. In the present study, pacidusin B, dichapetalin-type triterpenoid from Phyllanthus acidus (L.) Skeels (Euphorbiaceae), induces in HT1080 human fibrosarcoma line. Cells treated with B exhibited morphological characteristic 'ballooning' phenotype ferroptosis. The biochemical hallmarks were also observed B-treated cells. Both oxidative stress and ER play significant roles B-induced activation PERK-Nrf2-HO-1 signaling pathway led iron overload, while inhibition GPX4 further sensitized cancer Furthermore, molecular docking study showed that docked same pocket xCT as inducer erastin. These results revealed exerts anticancer effects via inducing ER-mediated ferroptotic death.

Язык: Английский

Процитировано

3
Sophora alopecuroide - Taraxacum Decoction (STD) inhibits non-small cell lung cancer via inducing ferroptosis and modulating tumor immune microenvironment DOI Creative Commons

Ouyang Xiaohu,

Jingbo Wang, Xiaoyuan Qiu

и другие.

Heliyon, Год журнала: 2024, Номер 10(20), С. e39564 - e39564

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

3
Metabolic reprogramming in lung cancer and its clinical implication DOI Creative Commons
Qingqiu Huang, Lisha Fan, Ming Gong

и другие.

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Дек. 18, 2024

Lung cancer has posed a significant challenge to global health, and related study been hot topic in oncology. This article focuses on metabolic reprogramming of lung cells, process adapt energy demands biosynthetic needs, supporting the proliferation development tumor cells. In this study, latest studies metabolism were reviewed, including impact products enzymes occurrence cancer, as well progress field treatment targeting relevant pathways. provides some promising potential directions into exploring helps researchers better understand cancer.

Язык: Английский

Процитировано

3
Multiomic machine learning on lactylation for molecular typing and prognosis of lung adenocarcinoma DOI Creative Commons

Mengmeng Hua,

Tao Li

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 24, 2025

To integrate machine learning and multiomic data on lactylation-related genes (LRGs) for molecular typing prognosis prediction in lung adenocarcinoma (LUAD). LRG mRNA long non-coding RNA transcriptomes, epigenetic methylation data, somatic mutation from The Cancer Genome Atlas LUAD cohort were analyzed to identify lactylation cancer subtypes (CSs) using 10 multiomics ensemble clustering techniques. findings then validated the GSE31210 GSE13213 cohorts. A model was developed identified hub LRGs divide patients into high- low-risk groups. effectiveness of this validated. We two CSs, which Nine LRGs, namely HNRNPC, PPIA, BZW1, GAPDH, H2AFZ, RAN, KIF2C, RACGAP1, WBP11, used construct model. In subsequent validation, high-risk group included more with stage T3 + 4, N1 2 3, M1, III IV cancer; higher recurrence/metastasis rates; lower 1, 5 year overall survival rates. oncogenic pathway analysis, most mutations detected group. tumor microenvironment analysis illustrated that immune activity notably elevated patients, indicating they might strongly respond immunotherapy than patients. Further, oncoPredict revealed have increased sensitivity chemotherapeutics. Overall, we a combines prognosis. Our represent valuable reference further understanding important function modification pathways progression.

Язык: Английский

Процитировано

0
Comprehensive exploration of programmed cell death landscape in lung adenocarcinoma combining multi-omic analysis and experimental verification DOI Creative Commons
Peng Yu,

Leyang Xiao,

Kaibo Hu

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 13, 2025

The mortality and therapeutic failure in lung adenocarcinoma (LUAD) are mainly resulted from the wide metastasis chemotherapy resistance. Up to now, accurate stable predictive prognostic indicator for revealing progress novel strategies of LUAD is infrequent, nonetheless. Diversified programmed cell death (PCD) has been widely confirmed that participated occurrence development various malignant tumors, respectively. In this research, we integrated fourteen types PCD, bulk multi-omic data TCGA-LUAD other cohorts gene expression omnibus (GEO) clinical patients develop our analysis. Consequently, pivotal PCD genes, especially CAMP, CDK5R1, CTSW, DAPK2, GAB2, GAPDH, GATA2, HGF, MAPT, NAPSA, NUPR1, PIK3CG, PLA2G3, SLC7A11, were utilized establish signature, namely index (CDI). validation several external indicated CDI can be regarded as a potential risk factor patients. Combined with common information, nomogram ability was constructed. Besides, according tumor microenvironment (TME) sensitivity some chemotherapeutic drugs further deeply explored. Notably, verification functional experiments demonstrated remarkable correlation between unfold protein response. Given all above, signature predict prognosis exploit precision

Язык: Английский

Процитировано

0
Integrated genomic analysis of the stemness index signature of mRNA expression predicts lung adenocarcinoma prognosis and immune landscape DOI Creative Commons
Xiaoling Lü, Wei Du, Jianping Zhou

и другие.

PeerJ, Год журнала: 2025, Номер 13, С. e18945 - e18945

Опубликована: Фев. 13, 2025

mRNA expression-based stemness index (mRNAsi) has been used for prognostic assessment in various cancers, but its application lung adenocarcinoma (LUAD) is limited, which the focus of this study. Low mRNAsi LUAD predicted a better prognosis. Eight genes ( GNG7, EIF5A, ANLN, FKBP4, GAPDH, GNPNAT1, E2F7, CISH ) associated with were screened to establish risk model. The differentially expressed between high and low groups mainly enriched metabolism, cell cycle functions pathway. score group had higher immune scores. Patients lower TIDE scores immunotherapy outcomes. In addition, was effective assessing drug sensitivity LUAD. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) data showed that eight lines, knockdown EIF5A reduced invasion migration ability cells. This study designed model based on mRNAsi-related predicting accurately prognosis survival patients, facilitating patients chemotherapy.

Язык: Английский

Процитировано

0
Discovery of Key Candidate Protein Biomarkers in Early-Stage Nonsmall Cell Lung Carcinoma through Quantitative Proteomics DOI

Baby Rorielyn T. Dimayacyac-Esleta,

Ferdinand D. Mira,

Lorenzo M. Zarate

и другие.

Journal of Proteome Research, Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

Difficulties in early-stage diagnosis are among the factors contributing to high mortality of nonsmall cell lung carcinoma (NSCLC) patients. Unfortunately, diagnostic biomarkers currently lacking, limiting options clinic. To discover proteins that have potential for biomarker applications, we performed an in-depth quantitative proteomic analysis on a cohort Filipino NSCLC adenocarcinoma (LUAD) Differentially expressed (DEPs) were obtained by using tandem mass tag (TMT) labeling and spectrometry (MS)-based proteomics. A total 6240 quantified identified with 3155 significantly upregulated 1248 downregulated. Integration result curated transcriptome data allowed identification 33 potential. This study also provided insights into relevant pathways LUAD, such as protein translation metabolic pathways. Interestingly, all enzymes hexosamine biosynthetic pathway (HBP) found be upregulated, suggesting its important role LUAD. It is worthwhile look at targeting vulnerability LUAD new strategy drug development. All MS deposited ProteomeXchange identifier PXD050598.

Язык: Английский

Процитировано

0