The Impact of Targeted Therapies on Red Blood Cell Aggregation in Patients with Chronic Lymphocytic Leukemia Evaluated Using Software Image Flow Analysis

Micromachines, Год журнала: 2025, Номер 16(1), С. 95 - 95

Опубликована: Янв. 15, 2025

Chronic lymphocytic leukemia (CLL), the most common type of leukemia, remains incurable with conventional therapy. Despite advances in therapies targeting Bruton's tyrosine kinase and anti-apoptotic protein BCL-2, little is known about their effect on red blood cell (RBC) aggregation flow. In this study, we applied a microfluidic device newly developed Software Image Flow Analysis to assess extent RBC CLL patients elucidate hemorheological effects commonly therapeutics Obinutuzumab/Venetoclax Ibrutinib. The results revealed that, samples from untreated patients, complex 3D clusters large aggregates are formed, number significantly increased compared healthy control samples. application combination did not affect aspect RBCs' rheological behavior. contrast, targeted therapy Ibrutinib preserves state RBCs levels seen controls, demonstrating that mitigates alterations properties associated CLL. Our findings highlight impact different properties, which critical for predicting potential complications side treatments, particularly concerning flow dynamics.

Язык: Английский

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Enhanced detection of albumin-creatinine ratio in urine using gold nanoparticle-integrated 3D-connector microfluidic paper-based analytical devices for early diagnosis of chronic kidney disease

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113071 - 113071

Опубликована: Фев. 1, 2025

Язык: Английский

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Dynamic Microfluidic Synthesis of Zinc Oxide Nanowires: Impact of Channel Architecture on Growth Homogeneity and Uniformity

Advanced Materials Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Фев. 19, 2025

Abstract Zinc oxide nanowires are synthesized in situ within microfluidic reactors dynamic mode owing to the continuous flow of growth solution. The synergistic effect fluid and confined volume enables fast synthesis, 8–16 min only, while 2–3 h needed static synthesis. However, co‐integration nanomaterials into poses challenges for their use as functional devices. Here, issue homogeneity nanowire well corresponding uniformity dimensions addressed. This is demonstrated that optimized tree‐branched microchannel networks excellent homogeneity, quantified by a surface coverage 99% across whole area reactor chamber, it limited 55%–78% when using conventional chambers. latter also leads severe non‐uniformity nanowires, eventually resulting radical changes morphology. On contrary, microchannels lead outstanding nanowires: average diameters 35 nm almost constant ± 1 chamber; length 420 varies 12 only. proposed approach applicable wide variety other

Язык: Английский

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High throughput microparticle production using microfabricated nozzle array

RSC Advances, Год журнала: 2025, Номер 15(9), С. 6823 - 6832

Опубликована: Янв. 1, 2025

Microparticle production system utilizing a microfabricated nozzle array and piezoelectric technology enables precise scalable polymeric microparticle production, offering significant improvements in drug delivery systems.

Язык: Английский

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The hidden power of glycine: A small amino acid with huge potential for piezoelectric and piezo-triboelectric nanogenerators

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 161514 - 161514

Опубликована: Март 1, 2025

Язык: Английский

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Reticular framework materials-enhanced biomimetic cascade catalysis systems for boosting advanced biosensing

Coordination Chemistry Reviews, Год журнала: 2025, Номер 534, С. 216571 - 216571

Опубликована: Март 15, 2025

Язык: Английский

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Fiber-assisted nanoparticle tracking analysis meets nanorheology: a novel approach for probing viscoelastic properties at the nanoscale

Nanophotonics, Год журнала: 2025, Номер unknown

Опубликована: Март 24, 2025

Abstract This study introduces fiber-assisted nanoparticle tracking analysis (FaNTA) as a platform for nanorheology that utilizes an advanced antiresonant optical fiber to analyze the viscoelastic properties of fluids at nanoscale. The confines colloidal nanotracers within fiber-integrated microchannel, significantly extending observation times and improving statistical accuracy. FaNTA system consists custom-designed microstructured fiber, dedicated setup, sophisticated data processing including image filtering, enabling precise determination hydrodynamic diameter thus local viscosity. demonstrates capabilities concept in context rheology by measuring viscosity glycerol-water solutions different concentrations using 50 nm gold nanospheres nanoprobes. By analyzing their individual diffusive motion, accurately determines fluid viscosities with results closely match literature values, validating efficacy nanorheological applications. FaNTA’s high accuracy performance nano- microrheological measurements highlight its broad potential nanoscale materials science, dynamic process studies, life environmental sciences, nanochemistry. innovative approach provides valuable extension current methods offers characterization wide range

Язык: Английский

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Microfluidic Optimization of PEI-Lipid Hybrid Nanoparticles for Efficient DNA Delivery and Transgene Expression

Pharmaceutics, Год журнала: 2025, Номер 17(4), С. 454 - 454

Опубликована: Апрель 1, 2025

Background: Lipid nanoparticles (LNPs) and polyethyleneimine (PEI) have independently been used for DNA complexation delivery. However, non-ideal gene delivery efficiency toxicity hindered their clinical translation. We developed DNA-PEI-LNPs as a strategy to overcome these limitations enhance transgene expression. Methods: Three microfluidic mixing protocols were evaluated: (i) LNPs without PEI, (ii) single-step process incorporating PEI in the organic phase, (iii) two-step with pre-complexed before LNP incorporation. The influence of DNA/PEI ratios (1:1, 1:2, 1:3) DNA/lipid (1:10, 1:40) on particle properties was examined. Results: In luciferase formulations, higher (1:40) produced smaller particles (136 nm vs. 188 nm) improved cellular uptake (77% 50%). method transfection efficiency, LNP-Luc/PEI 1:3 (40) achieving ~1.9 × 106 relative light units (RLU) green fluorescent protein (GFP) studies, LNP-GFP/PEI showed ~23.8% GFP-positive cells, nearly twofold than LNP-GFP at ~12.6%. Conclusions: These results demonstrate capability microfluidic-prepared improve expression through optimized formulation strategies selection appropriate preparation methods.

Язык: Английский

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Engineered ATP-Loaded Extracellular Vesicles Derived from Mesenchymal Stromal Cells: A Novel Strategy to Counteract Cell ATP Depletion in an In Vitro Model

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3424 - 3424

Опубликована: Апрель 5, 2025

The use of adenosine triphosphate (ATP) has shown promising effects in alleviating ischemic damage across various tissues. However, the penetration ATP into kidney tubular cells presents a challenge due to their unique anatomical and physiological properties. In this study, we introduce novel bioinspired drug delivery system utilizing extracellular vesicles (EVs) derived from mesenchymal stromal (MSCs) engineered carry ATP. ATP-loaded liposomes (ATP-LPs) EVs (ATP-EVs) were prepared using microfluidic technology, followed by characterization morphology (DLS, NTA, SEM, TEM), content, release rate at 37 °C (pH 7.4). Additionally, efficacy ATP-LPs ATP-EVs was evaluated vitro on renal (HK2 cells) under chemically induced ischemia. results indicated successful enrichment EVs, with showing no significant changes or size compared naïve EVs. Notably, demonstrated superior retention ATP-LPs, protecting degradation environment. an ATP-depleted HK2 cell model, only effectively restored levels, preserving viability reducing apoptotic gene expression (BCL2-BAX). This study is first successfully demonstrate direct as carriers.

Язык: Английский

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Antimicrobial Coatings Based on Hybrid Iron Oxide Nanoparticles

Nanomaterials, Год журнала: 2025, Номер 15(9), С. 637 - 637

Опубликована: Апрель 22, 2025

This study presents the preparation of hybrid iron oxide nanocomposites through a two-step process combining microfluidic-assisted synthesis and post-synthetic surface modification. Fe3O4 nanoparticles were synthesized simultaneously functionalized with salicylic acid using three-dimensional vortex-type microfluidic chip, enabling rapid uniform particle formation. The resulting Fe3O4/SA nanostructures further modified either silver or copper to form enhanced antimicrobial functionality. These subsequently integrated into silica aerogel matrices dip-coating approach improve dispersion, structural stability, biocompatibility. morphological properties samples investigated XRD, FT-IR, TEM SAED analysis, Raman microscopy. In vitro cytotoxicity assays demonstrated that Fe3O4/SA–Ag Fe3O4/SA–CuO exhibit potent antibacterial activity cell type-dependent vivo biodistribution studies showed no accumulation in major organs selective clearance via spleen, validating systemic safety platform. findings highlight potential as biocompatible, coatings for advanced biomedical surfaces.

Язык: Английский

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