
Journal of Bioenergetics and Biomembranes, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 5, 2024
Abstract Bacillus licheniformis can use cyanide as a nitrogen source for its growth. However, it also carry out aerobic respiration in the presence of this compound, classic inhibitor mammalian cytochrome c oxidase, indicating that B. has branched respiratory chain with various terminal oxidases. Here, we studied modifications when cells were cultured Nutrient Broth, an alkaline medium ammonium, or cyanide. Then, measured oxygen consumption intact and membranes, enzyme activities, carried 1D 2D-BN-PAGE, followed by mass spectrometry analysis BN-PAGE bands associated NADH, NADPH, succinate dehydrogenase activities. We found cell growth was favored nutrient than In parallel, activity progressively decreased rich medium, lowest growing membranes contain dehydrogenases, proteomic detected nitrate reductase bc, caa3, aa3, bd complexes. The migrated molecular 375 kDa, association (115 kDa + 241 respectively). NADH forms aggregates different mass.
Язык: Английский