Fusarium oxysporum assisted green synthesis of small-sized silver nanoparticles for high antibacterial, and photocatalytic decolorization performances

BMC Microbiology, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 6, 2025

Abstract Background Novel platforms using nanotechnology-based medicines have exponentially increased in our daily lives. The unique characteristics of metal oxide and noble metals nanoparticles make them suitable for different fields including antimicrobial agents, cosmetics, textiles, wound dressings, anticancer drug carriers. Methods This study focuses on the biosynthesis small-sized SNPs exo-metabolites Fusarium oxysporum via bioprocess optimization Plackett-Burman (PBD) central composite designs (CCD) while evaluating their multifaceted bioactivities. Results successful biofabrication smaller-sized with an average particle size ~ 5 nm was achieved upon optimization. developed exhibited significant antibacterial activity against multidrug-resistant bacterial pathogens a concentration- time-dependent manner. minimum inhibitory concentrations (MICs) were 0.078 µg/ml ( Escherichia coli ), 0.156 Pseudomonas aeruginosa 1.25 Enterococcus faecalis bactericidal (MBCs) correspondingly µg/ml, 0.312 µg/l, µg/ml. SNPs-treated cells displayed bacteriostatic effects as revealed by time-kill assay ultrastructure changes observed SEM TEM analyses. results marked potent antioxidant DPPH, O 2 •− , H OH-radicals IC 50 values 74.3, 96.7, 116.6, 167.9 respectively. Significantly, biosynthesized cytotoxic MCF-7, A549, HepG-2 cell lines 89.4, 121.4, 138.9 promising photocatalytic efficiency at times compared dark conditions. highest decolorization percentage crystal violet dye 98.60% after 240 min 100 µg concentration. Conclusions green synthesis F. exometabolites is eco-friendly, inexpensive, production small-size, greatly stabilized nanoparticles. corroborated that can be highly enough to applied control systems, ameliorating free radical-related disorders, photocatalyst wastewater treatment.

Язык: Английский

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Nanotechnology in food safety and preservation

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 233 - 278

Опубликована: Янв. 1, 2025

Язык: Английский

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Green synthesis, characterization, and antimicrobial activity of silver nanoparticles from water-soluble fractions of Brazilian Kefir

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 27, 2025

Nanotechnology offers promising new avenues for combating drug-resistant pathogens. Given its antioxidant capacity, the water-soluble fraction of Brazilian kefir was hypothesized to serve as an effective reducing agent green synthesis silver nanoparticles (AgNPs). It further that both entire (WSF) and < 10 kDa AgNPs would augment therapeutic effects kefir, particularly antimicrobial activity. The successful confirmed through use UV-Visible spectroscopy Fourier-transform infrared analyses. WSF-AgNPs demonstrated potent activity, with minimum inhibitory concentrations 25 µg/mL against A. baumannii (p 0.0001) 50 K. pneumoniae 0.0001). Although no toxicity observed in long-term tests on adult Drosophila melanogaster, intake impaired larvae development. Oxidative stress analysis showed mild oxidative imbalance advanced oxidation protein products (AOPP), sulfhydryl, reduced glutathione (GSH) contents, alterations reactive oxygen species (ROS) quantities, ferric power (FRAP), catalase (CAT) These findings suggest kefir-derived may have potential infections. Future studies should focus enhancing specificity compound conjugation investigating broader applications, including disinfectants, wound healing, antibiotic

Язык: Английский

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Synthesis of Silver Nanoparticles from Pseudophegopteris microstegia (Hook.) Ching and Their Biological Activities against Tomato Gray Mold

Journal of Agriculture and Food Research, Год журнала: 2025, Номер unknown, С. 101871 - 101871

Опубликована: Март 1, 2025

Язык: Английский

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INVESTIGATING THE ANTI-CANCER PROPERTIES OF A NEWLY SYNTHESIZED METAL-QUERCETIN NANOCOMPLEX IN THE CONTEXT OF CERVICAL CANCER

Asian Journal of Pharmaceutical and Clinical Research, Год журнала: 2025, Номер unknown, С. 223 - 229

Опубликована: Апрель 7, 2025

Objectives: This study aims to develop and evaluate quercetin-loaded silver nanoparticles (Q-AgNPs) as a potential therapeutic agent against cervical cancer, assessing their cytotoxic effects on HeLa cell lines. Methods: Quercetin was synthesized into AgNPs using bioreduction method involving nitrate, with subsequent characterization performed ultraviolet (UV)-Visible spectroscopy, Fourier Transform Infrared X-ray diffraction, scanning electron microscopy. The cytotoxicity of Q-AgNPs evaluated in vitro cancer cells the MTT assay determine viability calculate IC50 value. Results: revealed successful synthesis, distinctive peaks observed UV-Visible spectroscopy confirming interaction between quercetin silver. particle size analysis indicated range sizes, an entrapment efficiency 85.4%. assessment demonstrated dose-dependent decrease viability, value calculated at 11.765 μg/mL, indicating significant effects. Conclusion: findings highlight promising innovative approach for therapy. Further optimization vivo studies are warranted explore mechanisms action enhance efficacy this nanoparticle formulation.

Язык: Английский

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Biosynthesis of Curcuma longa-mediated silver nanoparticles for enhanced antibacterial efficacy

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Апрель 28, 2025

An economical and environment-friendly biosynthesis of silver nanoparticles (AgNPs) was attained utilizing an aqueous extract the rhizome Curcuma longa (Cl). Comprehensive characterization Cl-AgNPs performed employing relevant analytical tools, such as Fourier-transform infrared spectroscopy (FTIR), UV-visible spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD), scanning electron microscopy (SEM), Energy-dispersive analysis (EDAX) with elemental mapping, transmission (TEM). The UV-Vis indicated effective synthesis Cl-AgNPs, by alteration in surface plasmon resonance (SPR) peak at λ_max 416 (Cl extract) to λ_max 423 nm (Cl-AgNPs). were observed be spherical, 30-70 average size, exhibited a face-centred cubic (fcc) crystalline structure, evident XRD analysis. crystallite size determined 16 via Debye-Scherrer equation. antibacterial potential measured using broth dilution method establish minimum inhibitory concentration (MIC) maximum bactericidal (MBC), well well-diffusion technique 1 mg/ml concentration. demonstrated efficiency against Escherichia coli and Staphylococcus aureus, with inhibition zones measuring 8 mm and 6 mm, respectively. Additionally, concentration-dependent total antioxidant capacity compared standard (ascorbic acid) Cl extract. These findings indicate that hold promising for applications biomedicine nanomedicine.

Язык: Английский

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Silver Nanoparticles (AgNPs) from Lysinibacillus sp. Culture Broths: Antibacterial Activity, Mechanism Insights, and Synergy with Classical Antibiotics

Biomolecules, Год журнала: 2025, Номер 15(5), С. 731 - 731

Опубликована: Май 16, 2025

Antibiotic-resistant bacteria pose problems for infection prevention and treatment, so developing new procedures or substances against is mandatory. Silver nanomaterials are among the more promising antibacterial agents. Herein, we describe biogenic synthesis of silver nanoparticles (AgNPs) using culture broths from an undescribed species Lysinibacillus. Culture with without NaCl exponential stationary growth phases produced four AgNP types. Nanoparticles' shapes were quasi-spherical, core sizes 7.5-14.7 nm hydrodynamic diameters 48.5-80.2 nm. All AgNPs contained Ag0 crystals some AgCl ones. Moreover, their coronas presented different proportions carbohydrates, proteins, aliphatic compounds. The good agents six bacterial species, three Gram-positive Gram-negative, MICs 0.3-9.0 µg/mL. Their activity was higher Gram-negative particularly Pseudomonas aeruginosa. These acted synergistically several fifteen tested antibiotics. Interestingly, combinations these inhibited antibiotic-resistant bacteria, as in case S. epidermidis streptomycin aureus colistin. ROS production by E. coli when treated most suggested mechanisms killing depending on AgNP.

Язык: Английский

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WITHDRAWN: Biosynthesis of Curcuma longa-mediated silver nanoparticles for enhanced antibacterial efficacy

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Май 19, 2025

The full text of this preprint has been withdrawn by the authors in order to comply with an institutional policy on preprints. Therefore, do not wish work be cited as a reference.

Язык: Английский

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Phenotypic and Genotypic Characterization of Recently Isolated Multidrug-Resistant Acinetobacter baumannii Clinical and Aquatic Strains and Demonstration of Silver Nanoparticle Potency

Microorganisms, Год журнала: 2023, Номер 11(10), С. 2439 - 2439

Опубликована: Сен. 28, 2023

This study aims to demonstrate the effectiveness of silver nanoparticles (Ag NPs) on multidrug-resistant (MDR) Acinetobacter baumannii (AB) strains isolated from clinical and aquatic environment. Three types Ag NPs were investigated for their antimicrobial, antibiofilm, antivirulence properties a total number 132 AB in same temporal sequence intra-hospital infections (IHIs), wastewater (WW), surface water (SW) samples between 2019 2022 different Romanian locations characterized at phenotypic genotypic levels. The comparative analysis antimicrobial resistance (AR) profiles according isolation source geographical location demonstrated decrease MDR level recovered WW north-eastern/central/southern regions (N-E/C-W/analyzed S): 87.5/60/32.5%. lecithinase, caseinase, amylase, lipase producers, had variable biofilm formation ability, belonged six genotypes associated with presence virulence genes (ompA, csuE, bap, bfmS). synthesized solvothermal method exhibited an inhibitory effect microbial growth, adherence capacity inert substratum, production soluble factors. We report here first description powerful antibacterial agent against circulating hospitals anthropically polluted Romania.

Язык: Английский

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Green Synthesis of Silver Nanoparticles Using Extracts of Cocculus Pendulus: Morphology and Antibacterial Efficacy Against Common Nosocomial Pathogens

Indian Journal of Microbiology, Год журнала: 2024, Номер 64(4), С. 1598 - 1607

Опубликована: Март 20, 2024

Язык: Английский

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