Anti-Candida activity and biocompatibility of silver nanoparticles associated with denture glaze: a new approach to the management of denture stomatitis

Folia Microbiologica, Год журнала: 2024, Номер 69(6), С. 1229 - 1246

Опубликована: Апрель 23, 2024

Язык: Английский

---

In vitro antimicrobial activity of silver nanoparticles against selected Gram-negative and Gram-positive pathogens

Medicine and Pharmacy Reports, Год журнала: 2024, Номер 97(3), С. 280 - 297

Опубликована: Июнь 11, 2024

Background and aim. Infections caused by pathogenic bacteria increase patient morbidity mortality significantly raise treatment costs. The use of silver nanoparticles as an alternative for S aureus, E coli, MRSA, faecalis, K pneumoniae P aeruginosa indicates their antibacterial effect prompts medical research to consider the next generation drugs that could change antibiotic therapy. By combining with different classes antibiotics, is evidenced increased values inhibition zone compared obtained some antibiotics commonly used in bacterial infections. This study focuses on comparing activity versus combined against various bacteria, zones both. We aim prove size greater, thus confirming improved effect. Metods. In this we tested solutions alone or combination antibiotics. standard strains, ATCC, both Gram positive Staphylococcus aureus ATCC 29213, Enterococcus faecalis 29212, well negative Escherichia coli 25922, Pseudomonas 27853, but also clinical isolates: a strain MRSA (Methicillin Resistant aureus) PDR (pan drug resistant) Klebsiella pneumoniae. Bacterial identification was performed using Vitek MS analyzer (bioMerieux). Antibiotic susceptibility determination VITEK2 COMPACT SYSTEM (bio Merieux, Inc Durham NC) ready VITEK AST cards. interpretation results done compliance EUCAST 2023-2024 standards. Testing several nanoparticle 2 concentrations (10 μg/mL 100 μg/mL) combinations solutions. diameter (ZOI) nanoparticles, each bacterium expressed millimeters. Kirby-Bauer disk-diffusion method, accordance current standards, analyze at biocompatible doses 10 μg/mL. experiments were conducted triplicate, almost identical. Results. show displayed activity, proven appearance zone, sizes, all studied. beta-lactamins, first, second, third fourth cephalosporins, macrolides, fluoroquinolones, lincosamides, aminoglycosides, glycopeptides, tetracyclines, oxazolidinones, sulfonamides, rifamycins, amphenicols. highest demonstrated cephalosporins (32.6667 ± 0.701 mm), macrolides (31.6667 mm, lincosamides (29.6667 mm). (internal code GR0333), fluoroquinolones (36.3333 (32.3333 Fusid acid mm) aminoglicosides (31.3333 25922 Fosfomycine, 39 mm 29212 19 mm. For GQ8575) 12.3333 27253 16 1.214 Conclusions. metallic especially ones, antimicrobial agents definite bactericidal has led specialists new which may Studies vitro between represent highly efficient effective multidrug-resistant bacteria. To avoid problem resistance associated conventional it necessary understand adaptive mechanisms under action metal be exploited future studies. Further vivo studies would assess specify biocompatibility toxicity will make these super nanomaterials medicines future.

Язык: Английский

---

Fungus-mediated synthesis of Se-BiO-CuO multimetallic nanoparticles as a potential alternative antimicrobial against ESBL-producing Escherichia coli of veterinary origin

Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14

Опубликована: Март 22, 2024

Bacterial infections emerge as a significant contributor to mortality and morbidity worldwide. Emerging extended-spectrum β-lactamase (ESBL) Escherichia coli strains provide greater risk of bacteremia mortality, are increasingly resistant antibiotics, major producer ESBLs. E. bacteremia-linked mastitis is one the most common bacterial diseases in animals, which can affect quality milk damage organ functions. There an elevated menace treatment failure recurrence necessitating adoption rigorous alternative approaches. In this study, Se-Boil-CuO multimetallic nanoparticles (MMNPs) were synthesized alternate from Talaromyces haitouensis extract, their efficiency treating ESBL was confirmed using standard antimicrobial assays. Scanning electron microscopy, UV–visible spectroscopy, dynamic light scattering used validate characterize mycosynthesized Se-BiO-CuO MMNPs. spectra MMNPs showed absorption peak bands at 570, 376, 290 nm, respectively. The average diameters amorphous-shaped by T. extract approximately 66–80 (100 μg/mL) maximal inhibition zone 18.33 ± 0.57 mm against . also exhibited deleterious impact on killing kinetics, biofilm formation, swimming motility, efflux cellular components, membrane integrity. hemolysis assay confirms biocompatibility minimum inhibitory concentration (MIC) range. Our findings suggest that may serve potential substitute for bacteremia.

Язык: Английский

---

Candida parapsilosis CMGB-YT Biosurfactant for Treatment of Heavy Metal- and Microbial-Contaminated Wastewater

Processes, Год журнала: 2024, Номер 12(7), С. 1471 - 1471

Опубликована: Июль 13, 2024

During the last few decades, water pollution has become a growing concern at international level. To date, only Candida parapsilosis strains were successfully used in environmental remediation. In present article, strain C. CMGB-YT was studied for its ability to assimilate hydrophobic substrates and produce biosurfactants with antimicrobial activity positive effects on heavy metal removal from contaminated wastewaters. The grown yeast peptone (YP) media 1% n-decane, n-dodecane, n-tetradecane, n-hexadecane, as well commercial sunflower olive oils. production of biosurfactant evaluated using emulsification index (E24%). surface properties emulsifying stability determined. effect cell growth two Rhodotorula mucilaginosa their capacity lead (0.032 g/L) cadmium (0.030 ions synthetic wastewater also studied. potential 20 mg/mL 40 established presence pathogenic krusei strains. assimilated n-hexadecane good rates over 216 h produced an anionic glycolipidic stable E24% towards long-chain carbon compounds different temperatures, alkaline pH high salinity (10% NaCl). reduced tension 53.58 ± 0.42 mN/m, while critical micellar concentration (CMC) reached 4.2% biosurfactant. crude (5%) enhanced R. metal-contaminated wastewater, increased chemical oxygen demand (COD) up 80%, improved Cd2+ by 10%. Additionally, concentrated effectively prevented biofilm formation. conclusion, demonstrates promising efficient treatment metals microbial pathogens.

Язык: Английский

---

Biosynthesized silver nanoparticles as an Environmental-Friendly antibacterial nanosystem against Methicillin-resistant Staphylococcus aureus

Inorganic Chemistry Communications, Год журнала: 2024, Номер unknown, С. 113809 - 113809

Опубликована: Дек. 1, 2024

Язык: Английский

---