The Next Generation Fecal Microbiota Transplantation: To Transplant Bacteria or Virome

Advanced Science, Год журнала: 2023, Номер 10(35)

Опубликована: Ноя. 1, 2023

Abstract Fecal microbiota transplantation (FMT) has emerged as a promising therapeutic approach for dysbiosis‐related diseases. However, the clinical practice of crude fecal transplants presents limitations in terms acceptability and reproductivity. Consequently, two alternative solutions to FMT are developed: transplanting bacteria communities or virome. Advanced methods mainly include washed spores treatment. Transplanting virome is also explored, with development transplantation, which involves filtering from feces. These approaches provide more palatable options patients healthcare providers while minimizing research heterogeneity. In general, evolution next generation global trends components focuses on

Язык: Английский

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A guide to germ‐free and gnotobiotic mouse technology to study health and disease

FEBS Journal, Год журнала: 2024, Номер unknown

Опубликована: Март 24, 2024

The intestinal microbiota has major influence on human physiology and modulates health disease. Complex host–microbe interactions regulate various homeostatic processes, including metabolism immune function, while disturbances in composition (dysbiosis) are associated with a plethora of diseases believed to modulate disease initiation, progression therapy response. vast complexity the its metabolic output represents great challenge unraveling molecular basis specific physiological contexts. To increase our understanding these interactions, functional research using animal models reductionistic setting essential. In dynamic landscape gut research, use germ‐free gnotobiotic mouse technology, which causal disease‐driving mechanisms can be dissected, pivotal investigative tool for disease, dissected. A better health‐modulating functions opens perspectives improved therapies many diseases. this review, we discuss practical considerations design execution experiments, around rederivation housing conditions, route timing microbial administration, dosing protocols. This comprehensive overview aims provide researchers valuable insights experimental field research.

Язык: Английский

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An Overview of the Skin Microbiome, the Potential for Pathogen Shift, and Dysbiosis in Common Skin Pathologies

Microorganisms, Год журнала: 2025, Номер 13(1), С. 54 - 54

Опубликована: Янв. 1, 2025

Recent interest in the diverse ecosystem of bacteria, fungi, parasites, and viruses that make up skin microbiome has led to several studies investigating healthy a variety dermatological conditions. An imbalance normal flora can cause some diseases, current culture techniques are often unable detect microorganism further our understanding clinical–microbiological correlates disease dysbiosis. Atopic dermatitis rosacea presentations GPs manage may have an infective or microbiological component be challenging treat. We aim discuss implications including impact dysbiosis on conditions such as these. will also clinical pearls for initial future directions management atopic dermatitis, rosacea, hidradenitis suppurativa. Further research using culture-independent is needed involving microbial advance knowledge pathophysiology guide management.

Язык: Английский

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Skin Microbiome, Inflammation, and Skin Toxicities in Women with Breast Cancer Receiving Moderately Hypofractionated Radiation Therapy

International Journal of Radiation Oncology*Biology*Physics, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

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The Contribution of the Skin Microbiome to Psoriasis Pathogenesis and Its Implications for Therapeutic Strategies

Medicina, Год журнала: 2024, Номер 60(10), С. 1619 - 1619

Опубликована: Окт. 3, 2024

Psoriasis is a common chronic inflammatory skin disease, associated with significant morbidity and considerable negative impact on the patients' quality of life. The complex pathogenesis psoriasis still incompletely understood. Genetic predisposition, environmental factors like smoking, alcohol consumption, psychological stress, consumption certain drugs, mechanical trauma, as well specific immune dysfunctions, contribute to onset disease. Mounting evidence indicate that dysbiosis plays role in development exacerbation through loss tolerance commensal flora, an altered balance between Tregs effector cells, excessive Th1 Th17 polarization. While implications are only starting be revealed, progress characterization microbiome changes patients has opened whole new avenue research focusing modulation adjuvant treatment for part long-term plan prevent disease flares. may also represent valuable predictive marker response aid selection optimal personalized treatment. We present current knowledge results studies investigated efficacy different strategies management psoriasis, discuss interaction host flora.

Язык: Английский

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Comparison of the full-length sequence and sub-regions of 16S rRNA gene for skin microbiome profiling

mSystems, Год журнала: 2024, Номер 9(7)

Опубликована: Июнь 27, 2024

The skin microbiome plays a pivotal role in human health by providing protective and functional benefits. Furthermore, its inherent stability individual specificity present novel forensic applications. These aspects have sparked considerable research enthusiasm among scholars across various fields. However, the selection of specific 16S rRNA hypervariable regions for studies is not standardized should be validated through extensive tailored to different objectives targeted bacterial taxa. Notably, third-generation sequencing (TGS) technology leverages full discriminatory power gene enables more detailed accurate microbial community analyses. Here, we conducted full-length 141 microbiota samples from multiple anatomical sites using PacBio platform. Based on this data, generated derived sub-region data an

Язык: Английский

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Skin Microbiota: Mediator of Interactions Between Metabolic Disorders and Cutaneous Health and Disease

Microorganisms, Год журнала: 2025, Номер 13(1), С. 161 - 161

Опубликована: Янв. 14, 2025

Metabolic disorders, including type 2 diabetes mellitus (T2DM), obesity, and metabolic syndrome, are systemic conditions that profoundly impact the skin microbiota, a dynamic community of bacteria, fungi, viruses, mites essential for cutaneous health. Dysbiosis caused by dysfunction contributes to barrier disruption, immune dysregulation, increased susceptibility inflammatory diseases, psoriasis, atopic dermatitis, acne. For instance, hyperglycemia in T2DM leads formation advanced glycation end products (AGEs), which bind receptor AGEs (RAGE) on keratinocytes cells, promoting oxidative stress inflammation while facilitating Staphylococcus aureus colonization dermatitis. Similarly, obesity-induced dysregulation sebaceous lipid composition increases saturated fatty acids, favoring pathogenic strains Cutibacterium acnes, produce metabolites exacerbate Advances metabolomics microbiome sequencing have unveiled critical biomarkers, such as short-chain acids microbial signatures, predictive therapeutic outcomes. example, elevated butyrate levels psoriasis been associated with reduced Th17-mediated inflammation, presence specific Lactobacillus has shown potential modulate tolerance Furthermore, machine learning models increasingly used integrate multi-omics data, enabling personalized interventions. Emerging therapies, probiotics postbiotics, aim restore diversity, phage therapy selectively targets bacteria like without disrupting beneficial flora. Clinical trials demonstrated significant reductions lesions improved quality-of-life metrics patients receiving these microbiota-targeted treatments. This review synthesizes current evidence bidirectional interplay between disorders highlighting implications future directions. By addressing microbiota-mediated pathways, precision strategies paving way patient outcomes dermatologic care.

Язык: Английский

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Influence of Injectable Hyaluronic Gel System on Skin Microbiota, Skin Defense Mechanisms and Integrity (Ex vivo Study)

Clinical Cosmetic and Investigational Dermatology, Год журнала: 2025, Номер Volume 18, С. 459 - 473

Опубликована: Фев. 28, 2025

The influence of injectable hyaluronic gels on skin's microbiota is unclear. As well, skin a key factor modulating final effect gels. ex-vivo study was aimed at alterations following acid injection into the dermis in non-sterile surface conditions. Ex vivo human explants presence or absence either S. epidermidis aureus, were treated with control excipient (0.9% sodium chloride) test product (Hyaluronic S, HA-S). Bacterial analysis performed, as well structural integrity. Histological imaging and immunostaining markers: epidermal (CD1a, Toll-like receptor 2 (TLR2), Beta-defensin-3 (BD3), CCN1) dermal (DC-SIGN, Decorin) then performed. E P, both associated bacterial deposits, induced similar noticeable increase growth over 4 days, but no aureus. excipient, showed increased time. It observed significant CD1a, TLR2, CCN1 DC-SIGN, Decorin Day 2. product, contrast to very slight improvement viability decrease BD3, DC-SIGN Our investigation that intradermal injections, HA-based solution trigger short-term growth. We indicate strong conditions explant when barrier damaged by puncture highlights differences epidermal/dermal response depended injected composition.

Язык: Английский

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Advancing frontiers in skin offensive odor management: from innovative diagnostics to cutting-edge treatments and emerging technologies

Archives of Dermatological Research, Год журнала: 2025, Номер 317(1)

Опубликована: Март 8, 2025

Язык: Английский

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The relationship between the skin microbiome and probiotics in the healing of burn injuries

Folia Microbiologica, Год журнала: 2025, Номер unknown

Опубликована: Апрель 14, 2025

Язык: Английский

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