New quinazolone–sulfonate conjugates with an acetohydrazide linker as potential antimicrobial agents: design, synthesis and molecular docking simulations

RSC Advances, Год журнала: 2025, Номер 15(2), С. 1033 - 1048

Опубликована: Янв. 1, 2025

A new series of quinazolinone–alkanesulfonates have been designed and synthesized as antimicrobial agents. Two derivatives exhibited potential MIC activity.

Язык: Английский

---

Antibiotic Resistance Profile of E. coli Isolates from Lettuce, Poultry Manure, Irrigation Water, and Soil in Kumasi, Ghana

International Journal of Microbiology, Год журнала: 2024, Номер 2024, С. 1 - 10

Опубликована: Янв. 27, 2024

Inputs such as irrigation water and poultry manure used in lettuce cultivation have been found to be associated with antibiotic-resistant pathogens. The study assessed the antibiotic resistance profile of Escherichia coli isolated from lettuce, manure, water, soil Kumasi. One hundred fifty-six samples soil, were collected three farms over a seven-week period (seedlings harvest stage). E. enumerated using standard methods. 98% positive for coli. Geometric means ranged 2.0 × 105 1.67 107 MPN/100 ml while that 1.31 ml. Generally, microbial load all parameters on across weeks was significant exceeded World Health Organization (WHO) International Commission Microbiological Specifications Foods (ICMSF) recommendations food. Using Kirby Bauer method, sensitivity testing performed against 225 biochemically confirmed twelve antibiotics. Relatively high recorded some members beta-lactam class: meropenem: 94.2%, ampicillin: 91.9%, cefuroxime: 95.1%, ceftriaxone: 94.7%, cefotaxime: 94.2%. Eighty isolates screened extended spectrum beta lactamase (ESBL) production cefotaxime (CTX) cefotaxime/clavulanic acid (CTX/CLA) discs showed positive: one each soil. Polymerase chain reaction (PCR) presence blaCTX-M gene. occurrence vegetables their environment is alarming poses serious health threats general public. gene vegetable site first time Ghana requires enforcement by regulatory bodies inappropriate use antibiotics country.

Язык: Английский

---

Impacts of Bacillus probiotics on productive performance and egg quality criteria in laying Japanese quails

The Journal of Applied Poultry Research, Год журнала: 2024, Номер 33(3), С. 100445 - 100445

Опубликована: Май 22, 2024

The present study aimed to determine the effects of adding Bacillus megaterium and amyloliquefaciens laying quail diets on performance, egg quality, internal organ weights. We used 96 Japanese (Coturnix coturnix Japonica) at 12 weeks age. birds were divided into three treatment groups (32 quails per group), each eight replicates (four replicate). research was carried out in periods 28 days each. diets; 1st group served as a control group, receiving only basal diet. 2nd 3rd experimental received 0.1% (1×109 CFU/g) B. an addition diet, respectively. Adding did not influence performance parameters statistically. Also, significantly (p<0.05) impacted albumen index, Haugh unit, yolk compared group. In contrast, applied species affect other quality parameter values. body weight, heart liver gizzard small intestine length, caecum length among slaughter examined. CFU/g), can be without causing significant difference characteristics, visceral weights

Язык: Английский

---

Genomic profiling of extended-spectrum β-lactamase-producing Escherichia coli from Pets in the United Arab Emirates: Unveiling colistin resistance mediated by mcr-1.1 and its probable transmission from chicken meat – A One Health perspective

Journal of Infection and Public Health, Год журнала: 2023, Номер 16, С. 163 - 171

Опубликована: Ноя. 3, 2023

The United Arab Emirates (UAE) has witnessed rapid urbanization and a surge in pet ownership, sparking concerns about the possible transfer of antimicrobial resistance (AMR) from pets to humans environment. This study delves into whole-genome sequencing analysis ESBL-producing E. coli strains healthy cats dogs UAE, which exhibit multidrug (MDR). Additionally, it provides genomic exploration mobile colistin gene mcr-1.1, marking first instance its detection Middle Eastern pets.We investigate 17 UAE using WGS bioinformatics identify genes encoding virulence factors, assign diverse typing schemes isolates, scrutinize presence AMR genes. Furthermore, we characterized plasmid contigs housing mcr-1.1 conducted phylogenomic evaluate their relatedness previously identified isolates.Our unveiled variety factor-encoding within with fimH emerging as most prevalent. Regarding β-lactamase genes, blaCTX group 1 family predominated, CTX-M-15 found 52.9% (9/17) followed by CTX-M-55 29.4% (5/17). These isolates were categorized multiple sequence types (STs), epidemic ST131 being frequent. gene, linked resistance, was confirmed two isolates. belonged ST1011 displayed distinct profiles Phylogenomic revealed close connections between those chicken meat UAE.Our underscores MDR pets. identification mcr-1.1-carrying warrants urgency comprehensive surveillance highlights role companion animals epidemiology. findings underscore significance adopting One Health approach mitigate transmission risks effectively.

Язык: Английский

---

The Alternatives of Antibiotics in Poultry Production for Reducing Antimicrobial Resistance

World s Veterinary Journal, Год журнала: 2024, Номер 14(2), С. 270 - 283

Опубликована: Июнь 25, 2024

Antibiotics are natural, semi-synthetic, or chemical compounds that have anti-microbial activity and used in livestock poultry production for a variety of reasons, including therapeutic growth promotion. The use antibiotics has been associated with the development resistant bacteria. present study attempted to explain role as promoters, bacterial resistance, risks human health, special focus on some selected species isolated from farms products. Furthermore, manuscript reviewed literature alternative feed additives reduce reliance antibiotics. Microbial resistance is significant global health concern top threat 21st century. non-therapeutic promoters at low doses continuously, developing Meanwhile, antibiotic-resistant genes humans may their roots diets animals treated Developing encouraged researchers by identifying potential additives, such essential oils, bacteriophages, antimicrobial peptides, probiotics, prebiotics, organic acid, enzymes improve immune system functions, morbidity mortality, performances poultry, preserve consumer health.

Язык: Английский

---

In silico-designed antimicrobial peptide targeting MRSA and E. coli with antibacterial and antibiofilm actions

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Май 27, 2024

Abstract Antibiotic resistance is a paramount global health issue, with numerous bacterial strains continually fortifying their against diverse antibiotics. This surge in levels primarily stems from the overuse and misuse of antibiotics human, animal, environmental contexts. In this study, we advocate for exploring alternative molecules exhibiting antibacterial properties to counteract escalating antibiotic resistance. We identified synthetic antimicrobial peptide (AMP) by using computational search AMP public databases further engineering through molecular docking dynamics. Microbiological evaluation, cytotoxicity, genotoycity, hemolysis experiments were then performed. The designed underwent rigorous testing antibiofilm activities Methicillin-Resistant Staphylococcus aureus (MRSA) Escherichia coli ( E. ), representing gram-positive gram-negative bacteria, respectively. Subsequently, safety profile was assessed vitro human fibroblast cells blood sample. selected demonstrated robust efficacy MRSA , an added assurance non-cytotoxicity non-genotoxicity towards fibroblasts. Also, did not demonstrate any hemolytic activity. Our findings emphasize considerable promise as viable agent, showcasing its potential combat effectively.

Язык: Английский

---

A Decade of Antimicrobial Resistance in Human and Animal Campylobacter spp. Isolates

Antibiotics, Год журнала: 2024, Номер 13(9), С. 904 - 904

Опубликована: Сен. 21, 2024

Objectives: Campylobacter spp. remain a leading cause of bacterial gastroenteritis worldwide, with resistance to antibiotics posing significant challenges treatment and public health. This study examines profiles in antimicrobial (AMR) for isolates from human animal sources over the past decade. Methods: We conducted comprehensive review data studies spanning ten years, analyzing key antibiotics, ciprofloxacin (CIP), tetracycline (TET), erythromycin (ERY), chloramphenicol (CHL), gentamicin (GEN). Data were collated various regions assess global regional patterns resistance. Results: The analysis reveals concerning trend increasing patterns, particularly CIP TET, across multiple regions. While CHL GEN remains relatively low, high prevalence has significantly compromised options campylobacteriosis. Discrepancies observed between isolates, variations different continents countries. Notably, ERY showed variability, reflecting potential differences usage management practices. Conclusions: findings underscore ongoing challenge AMR Campylobacter, highlighting need continued surveillance research. rising prevalence, coupled discrepancies emphasize importance One Health approach address AMR. Enhanced monitoring, novel strategies, cooperation are crucial mitigating impact ensuring effective Campylobacter-related infections.

Язык: Английский

---

First National Study on Genomic Profiling of Escherichia Coli in Uae Aquatic Environments Shows Diverse Quinolone and Cephalosporin Resistance

Опубликована: Янв. 1, 2025

Язык: Английский

---

Systematic Review and Meta-Analysis on Prevalence and Antimicrobial Resistance Patterns of Important Foodborne Pathogens Isolated from Retail Chicken Meat and Associated Environments in India

Foods, Год журнала: 2025, Номер 14(4), С. 555 - 555

Опубликована: Фев. 7, 2025

The chicken value chain, a vital part of the global food supply, also represents significant public health concern due to risk foodborne pathogens, particularly in low- and middle-income countries (LMICs) such as India. This systematic review meta-analysis aimed assess prevalence bacterial pathogens including Salmonella spp., Campylobacter Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Clostridium perfringens, Klebsiella pneumonia. retail meat associated environments antimicrobial resistance based on articles published between January 2010-December 2023. research adhered guidelines 'Preferred Reporting Items for Systematic Review Meta-Analysis' (PRISMA). Based 90 included studies, S. aureus showed highest pooled (56%; 95% CI: 38-74%), followed by E. coli (50%; 37-64%), C. perfringens (35%; 10-65%), K. pneumoniae (21%; 7-38%). spp. (95% 11-26%) 11-27%) exhibited similar rates at 18%, while L. monocytogenes had lowest 13% 1-33%). A sensitivity analysis was subsequently conducted impact influential each pathogen recalculated after removing these studies ensure robustness results. specifically displayed high levels medically important antimicrobials (erythromycin, tetracycline, ciprofloxacin, colistin), potential threat human health. study advocates collaborative comprehensive approach, reflecting multifaceted nature issue, highlighting importance holistic strategy safeguard maintain antibiotic effectiveness face emerging challenges.

Язык: Английский

---

Prevalence, antibiotic resistance, virulence and antimicrobial resistance gene profiles of Salmonella species recovered from retail beef and poultry processing environments

BMC Microbiology, Год журнала: 2025, Номер 25(1)

Опубликована: Март 28, 2025

This study investigates the prevalence, antimicrobial resistance, genotypic resistance profiles, and virulence gene distribution of Salmonella isolates from poultry beef processing environments in Shahrekord, Iran. A total 680 samples were collected (n = 300) 380) products between January December 2023. was detected 21% (63/300) 15.8% (60/380) samples, with non-typhoidal (NTS) being predominant serovar. High (AMR) rates observed across both food types, most common resistances found ciprofloxacin (48%), tetracycline (44%), ampicillin (39%). Genotypic analysis revealed presence key genes, including blaTEM (35%), tetA (29%), sul1 (23%). Virulence identified invA (92%), agfA (80%), hilA (76%) as prevalent genes. Comparative patterns higher to tetracycline. highlights significant factors retail poultry, suggesting a potential risk public health need for enhanced surveillance control measures environments. Not applicable.

Язык: Английский

---