Exploration of bacterial composition and diversity within the apricot seed wasp Eurytoma samsonowi (Hymenoptera: Eurytomidae) by 16S rRNA Illumina sequencing

Symbiosis, Год журнала: 2025, Номер unknown

Опубликована: Янв. 16, 2025

Язык: Английский

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Investigation of host plant contact with Diaphorina citri by detecting D. citri‐derived environmental DNA

Entomologia Experimentalis et Applicata, Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

Abstract Citrus greening (Huanglongbing) disease has serious impacts on citrus production. Field monitoring of the Asian psyllid Diaphorina citri (Hemiptera: Psyllidae), a vector disease, is essential to prevent invasion and spread this disease. This study reports new method for determining presence D. traces contact with host plants by collecting leaves from survey area detecting environmental DNA (eDNA) derived . The results show that applied can be determined. As ‐derived eDNA contained in solutions history , we succeeded not only genes conserved mitochondria but also symbiont organisms consisting Wolbachia phage, Candidatus Carsonella sp., Profftella sp. could detected even after 10 min still 6 months contact. technology potential trace their without individual detection expected greatly contribute early warning future.

Язык: Английский

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Ontogenetic Analysis of Chelonus formosanus and Diversity of Its Internal Microbiota

Insects, Год журнала: 2025, Номер 16(2), С. 180 - 180

Опубликована: Фев. 8, 2025

Chelonus formosanus is a parasitic wasp capable of parasitizing various Noctuidae pests, including the highly invasive Spodoptera frugiperda, and it demonstrates strong pest control potential. Both egg larval stages primarily occur within host organism, total developmental time from to adult approximately 19.62 days. To investigate microbial communities at different stages, we performed 16S rDNA sequencing (V1–V9 region) using PacBio identified 404 bacterial species belonging 61 classes, 116 orders, 182 families, 308 genera across larval, pupal, female, male stages. Bacterial diversity richness varied with Enterobacter Enterococcus dominating in larvae pupae Pseudomonas emerging as dominant female adults. In contrast, adults predominantly resided Ralstonia Achromobacter. The predicted functions bacteria C. are marked by high abundance metabolic pathways. This study provides comprehensive understanding morphology contributes practical pests. Additionally, our findings preliminarily characterized community laying groundwork for its functional study.

Язык: Английский

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Insect-based flour: Exploring health benefits and consumer acceptance

Elsevier eBooks, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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The larval gut of Spodoptera frugiperda harbours culturable bacteria with metabolic versatility after insecticide exposure

Insect Molecular Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 14, 2025

Abstract Spodoptera frugiperda (fall armyworm) poses a substantial risk to crops worldwide, resulting in considerable economic damage. The gut microbiota of insects plays crucial roles digestion, nutrition, immunity, growth and, sometimes, the degradation insecticides. current study examines effect synthetic insecticides on microbiome third instar S. larvae using both culture‐dependent techniques and 16S rRNA gene sequencing for bacterial community profiling diversity analysis. In untreated larvae, approach revealed diverse dominated by phyla Firmicutes, Proteobacteria Bacteroidota, with key genera including Bacteroides , Faecalibacterium Pelomonas . parallel, 323 strains were isolated assigned orders Bacillales, Burkholderiales, Enterobacterales, Flavobacteriales, Lactobacillales, Micrococcales, Neisseriaies, Pseudomonadales, Sphingobacteriales Xanthomonadales. prevailing culturable species included Serratia marcescens Klebsiella variicola Enterobacter quasiroggenkampii Treatment sublethal concentrations three (broflanilide, spinosad indoxacarb) caused significant changes composition. Treated showed shift towards increased abundance decreased Firmicutes. Specifically, Acinetobacter Rhodococcus dominant treated samples. Functional predictions highlighted metabolic versatility involving nutrient processing, immune response, detoxification, xenobiotic metabolism, stress suggesting microbial adaptation insecticide exposure. Network correlation analysis disrupted interactions altered structures under treatment. These findings enhance our understanding how impact may inform future strategies managing pest resistance through microbiome‐based approaches.

Язык: Английский

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Future perspectives in the study of mutualistic interactions between insects and their microorganisms

Web Ecology, Год журнала: 2025, Номер 25(1), С. 39 - 45

Опубликована: Март 11, 2025

Abstract. The impact of climate change and habitat destruction on insect diversity survival is a critical area study. These disruptions could severely affect the symbiotic relationships between insects their microorganisms. Understanding how these interactions respond to such changes essential for mitigating decline populations, which are already alarmingly decreasing worldwide. Mutualistic microorganisms present vast opportunities in ecology, agriculture, biotechnology. associations crucial nutrition, defense, adaptation hold great potential developing antimicrobial compounds with promising applications pharmaceutical industry. In insect-associated play key role biological pest control, offering sustainable alternatives chemical pesticides. This would not only protect beneficial but also enhance agricultural efficiency. conservation mutualism must be integrated into biodiversity preservation efforts, as protecting will addressing future ecological challenges. following sections describe main mutualistic associated microorganisms, prospective approach directions that research should consider.

Язык: Английский

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Microplastics comprehensive review: Impact on honey bee, occurrence in honey and health risk evaluation

Journal of Applied Ecology, Год журнала: 2025, Номер unknown

Опубликована: Март 11, 2025

Abstract Microplastics (MPs), as an environmental contaminant, pose a significant risk to both animal and human health through the food water supply chains. Honey, widely recognised safe health‐oriented product, may become compromised if its production process involves non‐biodegradable MPs. This study was conducted systematic review, using comprehensive searches of PubMed, Scopus ScienceDirect investigate effects MP on honey bee health, potential route main species composition contamination in honey. review highlights impacts MPs including mortality, sucrose response, habituation, olfactory learning, memory recall, colony performance, body size growth, gut microbiota viral infection. From mechanistic perspective, can disrupt equilibrium microbiota, adversely impact function immune system, undermine neural signalling pathways that are critical for learning processes bees. It is crucial consider applied aspects these findings beekeeping practices, adopting sustainable practices mitigate exposure minimize production. The also provided detailed information contact routes with MPs, environment (air, water, soil, pollen), (plastic hive activities). affect by altering energy homeostasis, causing oxidative stress, system deficiencies, malnutrition, reduced growth decreased reproductive rates. Synthesis applications . this highly relevant industry public policymakers. By identifying key detrimental microplastics (MPs) quality, research provides actionable insights beekeepers adopt management minimise exposure. Additionally, underscores need regulatory policies control pollution, ensuring safety product protecting pollinators health.

Язык: Английский

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From nature to nurture: Shifts in the gut microbiota of the brown marmorated stink bug under laboratory rearing conditions

Entomologia Experimentalis et Applicata, Год журнала: 2025, Номер unknown

Опубликована: Март 24, 2025

Abstract Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) is an invasive pest for agricultural production. It associated with the primary symbiont “ Candidatus Pantoea carbekii”, which essential host's fitness. In a laboratory rearing, significant loss of fitness was observed, but reasons remained unknown. Since bacterial symbionts are known to have important impact on their hosts, we investigated gut microbiota laboratory‐reared H. individuals based 16S rRNA gene metabarcoding. We analyzed from different generations and compared field‐collected individuals. The results showed differences between natural insects. Especially, , most abundant taxon in field, lost replaced by unclassified Enterobacteriaceae Yersiniaceae . Our indicate that changes composition microbial community had negative influence highlight changing conditions insects consequences

Язык: Английский

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Parasite-Induced Replacement of Host Microbiota: Impact of Xenos gadagkari Parasitization on the Microbiota of Polistes wattii

Microbial Ecology, Год журнала: 2025, Номер 88(1)

Опубликована: Март 27, 2025

Язык: Английский

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Effects of different origins of two pesticides on several bacteria in the microbiota of Bombus terrestris L.

Mediterranean Agricultural Sciences, Год журнала: 2025, Номер 38(1), С. 15 - 20

Опубликована: Апрель 9, 2025

This study aimed to evaluate the effects of pesticides from different origins on several bacteria in microbiota Bombus terrestris. In experiments, recommended field doses (1/1) and sublethal (1/10, 1/100, 1/1000) Subtilex Foliar (Bacillus subtilis MBI 600, Bioglobal©) Pusula (Thiamethoxam, Hektaş©) were used. The experiments performed with 5 replications (10 worker bees per replication). fed sugar syrup + pesticide mixture prepared for days each replication. On fifth day after applications, random individuals trial selected live and, being mechanically euthanized, placed sterilized falcon tubes labeled experimental group details. same procedure was applied analysis control as groups. These then analyzed determine microorganism changes. According results obtained, colony development two species bacteria, Staphylococcus xylosus S. gallinarum, determined group. gallinarum completely inhibited 1/1 1/10 dose applications B. while dominant 600. became application 1/100 1/1000 dose, 600 could not colonize. When Thiamethoxam compared group, colonies Pseudomonas sp. who received application. show that used cause significant changes life.

Язык: Английский

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