Omics approaches in understanding the benefits of plant-microbe interactions

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Май 27, 2024

Plant-microbe interactions are pivotal for ecosystem dynamics and sustainable agriculture, influenced by various factors, such as host characteristics, environmental conditions, human activities. Omics technologies, including genomics, transcriptomics, proteomics, metabolomics, have revolutionized our understanding of these interactions. Genomics elucidates key genes, transcriptomics reveals gene expression dynamics, proteomics identifies essential proteins, metabolomics profiles small molecules, thereby offering a holistic perspective. This review synthesizes diverse microbial-plant interactions, showcasing the application omics in mechanisms, nitrogen fixation, systemic resistance induction, mycorrhizal association, pathogen-host Despite challenges data integration ethical considerations, approaches promise advancements precision intervention resilient agricultural practices. Future research should address challenges, enhance technology resolution, explore epigenomics, understand plant-microbe under conditions. In conclusion, technologies hold immense optimizing strategies fortifying alliances, paving way agriculture stewardship.

Язык: Английский

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Advanced rDNA-Based Detection of Wheat Pathogens in Grain Samples Using Next-Generation Sequencing (NGS)

Pathogens, Год журнала: 2025, Номер 14(2), С. 164 - 164

Опубликована: Фев. 7, 2025

High-throughput sequencing (HTS) has revolutionized phytopathology by overcoming many limitations of traditional diagnostic methods, as it permits precise pathogen monitoring, identification, and control, with ribosomal DNA (rDNA) regions serving reliable markers for fungal classification. In this study, next-generation (NGS) was used, targeting the ITS1 ITS2 to explore diversity presence in winter wheat grain samples identifying 183 OTU sequences across 115 taxa. The analysis yielded 249,743 reads, Fusarium sp. (61%) dominant pathogenic taxon, followed Sporobolomyces (14%), Cladosporium (3%), other yeast-like or saprotrophic fungi, such Cryptoccocus spp., F. wieringae, B. alba. Sequencing also permitted detection acuminatum quarantine-regulated pathogens T. caries triticoides. produced 179,675 culmorum (47%) most abundant confirming significant contamination pathogen. Other frequently detected taxa included fungi C. tephrensis (21%) V. victoriae (13%), along species like S. roseus Davidella provided better resolution identification more associated cereal diseases, including culmorum, well cerealis, poae, tricinctum. revealed a diverse community, A. porri, cinerea, herbarum, various non-pathogenic These findings underscore complementary utility profiling detecting critical using HTS, highlighting potential these monitoring managing crop health.

Язык: Английский

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Microbial genomics and genome sequencing approaches

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 3 - 20

Опубликована: Янв. 1, 2025

Язык: Английский

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Harnessing artificial intelligence to address diseases attributable to unsafe drinking water: challenges, potentials, and recommendations

Discover Water, Год журнала: 2025, Номер 5(1)

Опубликована: Март 13, 2025

Язык: Английский

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Metagenomics and plant-microbe symbioses: Microbial community dynamics, functional roles in carbon sequestration, nitrogen transformation, sulfur and phosphorus mobilization for sustainable soil health

Biotechnology Advances, Год журнала: 2025, Номер 82, С. 108580 - 108580

Опубликована: Апрель 15, 2025

Язык: Английский

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Novel Molecular Techniques for Identifying Agricultural Microorganisms

Agriculture, Год журнала: 2024, Номер 14(7), С. 987 - 987

Опубликована: Июнь 25, 2024

Agriculture involves activities aimed at improving soil quality for food production. In this environment, microorganisms play a vital role, positively and/or negatively affecting plant growth. Given impact, knowing the microbiota associated with agricultural systems and phytopathogens is crucial. The microbial culture method has proven ineffective in identifying systems, more effective methods greater scope their identification currently exist. This review compiles updated information on new studying system, such as metagenomics, proposals microorganism methods, Raman spectrometry, nanotechnology, phytopathogen biosensors. addition, it discusses strengths limitations of identification.

Язык: Английский

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Pathogenic and Harmful Bacteria in Dairy Technology: Genomic Characterization and Its Correlation with Physicochemical Parameters of Sheep’s Cheese Sold in Southern Brazil

Fermentation, Год журнала: 2024, Номер 10(8), С. 397 - 397

Опубликована: Июль 31, 2024

The distinct microbiological and physicochemical characteristics of sheep milk enable the production cheeses with unique organoleptic properties. scenario in Brazil is still uncertain, lacking data regulations. This study aims to characterize diversity bacterial groups potential pathogenic and/or harmful properties technology correlate presence these bacteria cheese. Additionally, it investigates virulence genes, resistance toxins reference genomes. main steps were (a) sample preparation fractionation; (b) analysis; (c) analysis free fatty acids profile by GC-FID; (d) volatile compounds GC-MS; (e) molecular analysis—Next Generation Sequencing 16S rRNA gene (V3-V4 region); (f) silico analysis—Genomics. A total 1,061,392 sequences identified, corresponding 45 genera 57 species. Of abundance, 95.7% considered beneficial, while 3.9% classified as (BPP) (BHMT). Five phyla identified: Firmicutes, Proteobacteria, Actinobacteriota, Bacteroidota, Fusobacteriota. Acinetobacter, Pseudomonas, Staphylococcus stood out BPP BHMT groups, showing higher abundance. Alpha cheese samples showed that origin significantly influences richness, evenness, abundance Some parameters, acids, organic a negative correlation Staphylococcus. Reference genomes two species exhibited number genes. reinforces need monitor lesser relevance prevent transmission, through food, potentially resistant virulent pathogens.

Язык: Английский

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Guarding our food: keeping it safe in a changing world through multidisciplinary approaches

Asian-Australasian Journal of Bioscience and Biotechnology, Год журнала: 2024, Номер 9(2), С. 10 - 13

Опубликована: Май 31, 2024

Abstract not available Asian Australas. J. Biosci. Biotechnol. 2024, 9(2), 10-13

Язык: Английский

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Insights to ballast water: metagenomics as a pressing priority

Опубликована: Июль 1, 2024

Metagenomics represents a pivotal development in genomics, offering unprecedented insights into diverse spectrum of microorganisms, including bacteria, viruses, and fungi, that were previously challenging or impossible to study outside natural environments. Its applications span remarkable range, from exploring cosmic entities the depths our oceans, incorporating numerous analyses. Yet, its utilization context ballast water analysis remains scarce. This highlights crux present review, which aims showcase need for metagenomics analysis. As is known, essential maintaining ship stability under no cargo conditions during adverse weather, with daily global movements estimated be between 3 10 billion tonnes. A single bulk can transport up 60,000 tonnes 200,000-tonne load. It over 7,000 species are transported globally ships' daily, posing significant ecological risks when these organisms discharged new environments where they become invasive species, disrupting local ecosystems, economies, human health. Despite International Maritime Organization setting D-2 standard guidelines endorsing several management practices safe discharge, issue persists. underscores necessity employing enhance monitoring microbial content water. The review summarizes some research conducted worldwide analyze by metagenomics, all exhibiting microbes, potent pathogenic bacterial viral forms. findings support view, making an invaluable tool monitor treatment effectiveness compliance evolving environmental regulations. Though challenges facing metagenomic applications—namely cost, complexity, comprehensive reference databases—are significant, will surely surmountable considering continual technological methodological advancements. Embracing offers pathway not only address threats posed transfers but also safeguard future shipping industries.

Язык: Английский

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Biocontrol potential of fungal endophytes for pests management in cereals

Biocontrol Science and Technology, Год журнала: 2024, Номер unknown, С. 1 - 42

Опубликована: Дек. 9, 2024

Cereal production is vital for food security and economic growth, but insect infestations can significantly reduce crop yields quality, leading to substantial losses. Recent pest management strategies in cereal crops have incorporated innovative techniques, such as utilizing mutualistic plant-microbe interactions that trigger molecular defense mechanisms against pests. Entomopathogenic fungi, particularly Beauveria Metarhizium (Hypocreales), are naturally found soil infect a wide range of arthropod These fungi also colonize plants rhizosphere inhabitants endophytes, offering multiple benefits, including enhanced growth resistance. As these promote plant biomass herbivore development, consumption, oviposition, survival the field. Species like bassiana anisopliae shown potential controlling both chewing sap-sucking pests major cereals wheat, rice, maize, sorghum. However, exact through which control endophytes not yet fully understood. This review summarizes biological entomopathogenic fungi-plant their role control, exploring how could be integrated into discussing challenges application. Future research should focus on understanding adaptive enable function endophytic genetics influence improve suppression. By uncovering mechanisms, we enhance effectiveness fungi-based contributing more sustainable crops.

Язык: Английский

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