Immobilization of alginate C-5 epimerases using Bacillus subtilis spore display DOI Creative Commons

Jan Benedict Spannenkrebs,

Agnes Beenfeldt Petersen, Finn L. Aachmann

и другие.

Applied and Environmental Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 3, 2025

Alginates are the most abundant polysaccharides found in brown seaweed, composed of (1→4)-linked β-D-mannuronate (M) and its C-5 epimer, α-L-guluronate (G). The G-blocks alginate possess viscosifying gelling properties, making alginates valuable industrial polysaccharides. Alginate epimerases enzymes epimerizing M to G, enhancing usability value alginate. three AlgE1, AlgE4, AlgE6 were immobilized using Bacillus subtilis spores displaying fused spore crust protein CotY. To our knowledge, this is first display alginate-modifying enzymes. Activity assays four AlgE4-displaying strains showed that AlgE4 produced MG-blocks from polyM was tested linked by N- C-termini. Two linkers with different flexibility tested, both containing a TEV protease cleavage site. Immobilizing on B. resulted recyclable system easy isolate reuse, thus opening possibilities for application. Recyclability demonstrated performing five consecutive reactions same batch spores, retaining 24% starting activity after rounds reuse. spore-displayed enzyme optimized green fluorescent protein, these conditions used cleave off spores. successful, but efficiency varied depending which terminus Seaweed scalable resource requires no fresh water, fertilizer, or arable land, it an important biomass bioeconomies. major component seaweed widely food, feed, technical, pharmacological industries. tailor functional properties alginates, have shown be promising postharvest valorization This study investigates efficient method produce epimerases, new use cases. In study, surface bacterium forms reaction nutrient starvation, highly resistant external influences can repurposed as stable platform numerous applications due ease genomic manipulation cultivation.

Язык: Английский

Immobilization of alginate C-5 epimerases using Bacillus subtilis spore display DOI Creative Commons

Jan Benedict Spannenkrebs,

Agnes Beenfeldt Petersen, Finn L. Aachmann

и другие.

Applied and Environmental Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 3, 2025

Alginates are the most abundant polysaccharides found in brown seaweed, composed of (1→4)-linked β-D-mannuronate (M) and its C-5 epimer, α-L-guluronate (G). The G-blocks alginate possess viscosifying gelling properties, making alginates valuable industrial polysaccharides. Alginate epimerases enzymes epimerizing M to G, enhancing usability value alginate. three AlgE1, AlgE4, AlgE6 were immobilized using Bacillus subtilis spores displaying fused spore crust protein CotY. To our knowledge, this is first display alginate-modifying enzymes. Activity assays four AlgE4-displaying strains showed that AlgE4 produced MG-blocks from polyM was tested linked by N- C-termini. Two linkers with different flexibility tested, both containing a TEV protease cleavage site. Immobilizing on B. resulted recyclable system easy isolate reuse, thus opening possibilities for application. Recyclability demonstrated performing five consecutive reactions same batch spores, retaining 24% starting activity after rounds reuse. spore-displayed enzyme optimized green fluorescent protein, these conditions used cleave off spores. successful, but efficiency varied depending which terminus Seaweed scalable resource requires no fresh water, fertilizer, or arable land, it an important biomass bioeconomies. major component seaweed widely food, feed, technical, pharmacological industries. tailor functional properties alginates, have shown be promising postharvest valorization This study investigates efficient method produce epimerases, new use cases. In study, surface bacterium forms reaction nutrient starvation, highly resistant external influences can repurposed as stable platform numerous applications due ease genomic manipulation cultivation.

Язык: Английский

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