Genomic Characterization of NDM‐1 Harboring Extensively‐Drug Resistance Klebsiella pneumoniae Isolate From ICU‐Admitted Patient With COVID‐19 DOI Creative Commons
Abolfazl Rafati Zomorodi, Himen Salimizand, Niloufar Mohseni

и другие.

Journal of Tropical Medicine, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

Currently, carbapenem‐resistant Klebsiella pneumoniae (CR‐KP) strains, particularly those producing New Delhi metallo‐beta‐lactamase (NDM), are increasingly recognized as a significant threat to global health. The present study aimed conduct genomic analysis of an NDM‐1‐producing CR‐KP strain isolated from patients with coronavirus disease 2019 (COVID‐19) admitted the intensive care unit (ICU). K. isolate was obtained bronchoalveolar lavage fluid 68 year‐old male patient hospitalized in ICU COVID‐19 at Besat Hospital Sanandaj, Iran. minimum inhibitory concentrations (MICs) for 15 antibiotics were determined using VITEK 2 system. Genomic performed whole genome sequencing. CRKP‐51 identified extensively drug‐resistant (XDR) strain, exhibiting resistance all tested except tigecycline (MIC = μg/mL). highest values recorded against sulfamethoxazole‐trimethoprim (SXT), nitrofurantoin (NIT), and piperacillin‐tazobactam (TZP), MICs ≥ 320, 256 μg/mL, 128 respectively. Multilocus sequence typing revealed that belonged type (ST15). IncHI1B replicon associated this harbored several genes, including b l N D M −1 , armA msrE mphE BRP (MBL), O X A aadA 2, dfrA 12, qnrB 1, C T − −15 cat 1. High‐risk clones, such ST15, antimicrobial emergence XDR strains ICUs. Additionally, dissemination NDM enzyme occurs through various plasmid types. Therefore, monitoring local epidemiology is essential effectiveness stewardship programs.

Язык: Английский

Prevalence and mechanisms of high-level carbapenem antibiotic tolerance in clinical isolates of Klebsiella pneumoniae DOI Creative Commons
Trevor Cross,

Facundo Torres,

Abigail P. McGee

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 19, 2025

Abstract Antibiotic tolerance is the ability of bacteria to survive normally lethal doses antibiotics for extended time periods. Clinically significant Enterobacterales, example, often exhibit high last-resort antibiotic meropenem. Meropenem associated with formation cell wall-deficient spheroplasts that readily recover rod shape and normal growth upon removal antibiotic. Both true prevalence tolerance, genetic mechanisms underlying it, remain poorly understood. Here, we find meropenem widespread among clinical Enterobacterales. Using forward genetics, uncover novel factors in a hypertolerant isolate ESKAPE pathogen Klebsiella pneumoniae . We multiple contribute envelope stress responses (PhoPQ, Cpx, Rcs OmpR/EnvZ) collectively promote spheroplast stability recovery, while lytic transglycosylase MltB counteracts it. Our data indicate isolates, outer membrane maintenance key factor promoting survival tolerant K.

Язык: Английский

Процитировано

0
Genomic Characterization of NDM‐1 Harboring Extensively‐Drug Resistance Klebsiella pneumoniae Isolate From ICU‐Admitted Patient With COVID‐19 DOI Creative Commons
Abolfazl Rafati Zomorodi, Himen Salimizand, Niloufar Mohseni

и другие.

Journal of Tropical Medicine, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

Currently, carbapenem‐resistant Klebsiella pneumoniae (CR‐KP) strains, particularly those producing New Delhi metallo‐beta‐lactamase (NDM), are increasingly recognized as a significant threat to global health. The present study aimed conduct genomic analysis of an NDM‐1‐producing CR‐KP strain isolated from patients with coronavirus disease 2019 (COVID‐19) admitted the intensive care unit (ICU). K. isolate was obtained bronchoalveolar lavage fluid 68 year‐old male patient hospitalized in ICU COVID‐19 at Besat Hospital Sanandaj, Iran. minimum inhibitory concentrations (MICs) for 15 antibiotics were determined using VITEK 2 system. Genomic performed whole genome sequencing. CRKP‐51 identified extensively drug‐resistant (XDR) strain, exhibiting resistance all tested except tigecycline (MIC = μg/mL). highest values recorded against sulfamethoxazole‐trimethoprim (SXT), nitrofurantoin (NIT), and piperacillin‐tazobactam (TZP), MICs ≥ 320, 256 μg/mL, 128 respectively. Multilocus sequence typing revealed that belonged type (ST15). IncHI1B replicon associated this harbored several genes, including b l N D M −1 , armA msrE mphE BRP (MBL), O X A aadA 2, dfrA 12, qnrB 1, C T − −15 cat 1. High‐risk clones, such ST15, antimicrobial emergence XDR strains ICUs. Additionally, dissemination NDM enzyme occurs through various plasmid types. Therefore, monitoring local epidemiology is essential effectiveness stewardship programs.

Язык: Английский

Процитировано

0