Transcriptome analysis of nitrogen assimilation preferences in Burkholderia sp. M6-3 and Arthrobacter sp. M7-15 DOI Creative Commons
Ran Liu, Hongyi Qin, Qian Wang

и другие.

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Апрель 7, 2025

Introduction Ammonium (NH 4 + ) and nitrate (NO 3 − are the two main forms of inorganic nitrogen (N) that exist in soil both can be absorbed utilized by plants. As a vast crucial biome, microorganisms responsible for mediating N assimilation process enhancing use efficiency. Understanding how these assimilate different is crucial. There handful play dominant role have significant advantage abundance. However, microbial preferences ammonium or nitrate, as well differences their metabolic pathways under co-existing conditions, remain unclear. Methods In this study, strains with advantages, Burkholderia sp. M6-3 Arthrobacter M7-15 were isolated from an acidic Chinese then incubated sources to investigate preferences. Furthermore, RNA sequencing-based transcriptome analysis was used map explore explanatory potential Results The results showed strain preferred utilize NH while NO . Although shared similar pathways, differential expression glutamine synthetase-coding gene glnA played regulating This inconsistency may attributed GlnR , global regulator utilization. Discussion research strengthens theoretical basis exploring underlying causes provided key clues screening functional ultimately enhance

Язык: Английский

The Mechanism of Ammonia-Assimilating Bacteria Promoting the Growth of Oyster Mushrooms (Pleurotus ostreatus) DOI Creative Commons
Rui Li, Qi Zhang,

Yuannan Chen

и другие.

Journal of Fungi, Год журнала: 2025, Номер 11(2), С. 130 - 130

Опубликована: Фев. 9, 2025

Oyster mushrooms (Pleurotus ostreatus) are one of the most commonly grown edible using compost, which contains high concentrations ammonia. In this study, inoculation oyster mushroom culture substrate with ammonia-assimilating bacterium Enterobacter sp. B12, either before or after composting, reduced ammonia nitrogen content, increased total content and enhanced yield. Co-cultivation P. ostreatus mycelia on potato dextrose agar (PDA) plates containing 200 mM NH4+, B12 reactive oxygen species (ROS) accumulation in downregulated expression ROS-generating enzymes NADPH oxidase A (NOXA) stress hormone ethylene synthase 1-aminocyclopropane-1-carboxylate (ACO). It also related genes mycelia, such as glutamate dehydrogenase (GDH), (GOGAT), glutamine synthetase (GS), transporter protein (AMT), amino acid (AAT), while upregulating its own ammonia-assimilation genes. These findings suggest that mechanism by promoted growth was assimilated ammonia, alleviated stress, mitigated ROS supplied acids to mycelia. To our knowledge, bacteria a novel type promoter (MGP).

Язык: Английский

Процитировано

0
Transcriptome analysis of nitrogen assimilation preferences in Burkholderia sp. M6-3 and Arthrobacter sp. M7-15 DOI Creative Commons
Ran Liu, Hongyi Qin, Qian Wang

и другие.

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Апрель 7, 2025

Introduction Ammonium (NH 4 + ) and nitrate (NO 3 − are the two main forms of inorganic nitrogen (N) that exist in soil both can be absorbed utilized by plants. As a vast crucial biome, microorganisms responsible for mediating N assimilation process enhancing use efficiency. Understanding how these assimilate different is crucial. There handful play dominant role have significant advantage abundance. However, microbial preferences ammonium or nitrate, as well differences their metabolic pathways under co-existing conditions, remain unclear. Methods In this study, strains with advantages, Burkholderia sp. M6-3 Arthrobacter M7-15 were isolated from an acidic Chinese then incubated sources to investigate preferences. Furthermore, RNA sequencing-based transcriptome analysis was used map explore explanatory potential Results The results showed strain preferred utilize NH while NO . Although shared similar pathways, differential expression glutamine synthetase-coding gene glnA played regulating This inconsistency may attributed GlnR , global regulator utilization. Discussion research strengthens theoretical basis exploring underlying causes provided key clues screening functional ultimately enhance

Язык: Английский

Процитировано

0